Korean journal of food and cookery science (한국식품조리과학회지)

Korean Society of Food and Cookery Science (한국식품조리과학회)
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Screening of Antioxidant Activity in Dansam(Salvia miltiorrhiza) and Additional Effect on the Shelf-Life and the Characteristics of Yakgwa

단삼추출물의 항산화능 검색 및 약과에의 첨가효과

  • 김윤화 (숙명여자대학교 식품영양학전공) ;
  • 한영실 (숙명여자대학교 식품영양학전공) ;
  • 백재은 (부천대학 식품영양과) ;
  • 송태희 (배화여자대학 식품영양과)
  • Published : 2003.08.01
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Abstract

This study was carried out to investigate the antioxidant activities of Dansam (S. miltiorrhiza). The Dansam (S. miltiorrhiza) was extracted with ethanol and methanol, and the extracts were fractionated with hexane, chloroform, ethyl acetate and butanol and water, in that order. The antioxidant activities of Dansam (S. miltiorrhiza) were determined by measuring the radical scavenging effects, using the 1, l-diphenyl-2-picryl-hydrazyl (DPPH) method. The electron donating ability was shown to be about 50% (IC50) at concentration of L-ascorbic acid, Dansam that reflected eliminatory effect by 50% were 9.48$\mu\textrm{g}$/$m\ell$, 8.28$\mu\textrm{g}$/$m\ell$, and l2.59$\mu\textrm{g}$/$m\ell$ respectively. According to the results of the above anti oxidation experiments, those for the group with the added Dansam showed a decreased oxidation, but the antioxidation increased with time. With a storage temperature of 60 C for 5 days, the acid value for the relative antioxidant activities were higher than in the Control group. The peroxide values for the relative antioxidant activities were also higher than in the Control group. The TBA values for the relative antioxidant activities were higher than in the Control group.

단삼을 ethanol로 추출하여 추출물을 얻고, methanol로 추출하여 용매별로 순차 분획하여 농축시켜 분획별 추출물을 얻었다. 항산화효과를 실행하기 위해 DPPH 방법으로 전자공여능을 측정하였다. 식품에의 첨가에 대한 보존효과를 실험하기 위해 약과에 단삼 에탄을 추출물을 0.0, 0.25, 0.5, 0.75, 1.0%별로 첨가하여, 제조 한 후 5일간 6$0^{\circ}C$의 Drying oven에 넣어 24시간 단위로 AV, POV, TBA가를 측정하였다. 전자공여능을 측정한 결과 단삼에탄을 추출물은 IC50이 8.27과 12.59$\mu\textrm{g}$/$m\ell$이었으며, 메탄을 추출물의 각 분획별 전자공여능은 Ethyl acetate층> Chloroform 층> Buthanol층> Water층> Hexane층 순으로 나타났다. 지질산패도 실험 결과, 단삼 첨가한 약과는 무첨가군에 비해 지질산화가 적게 일어났으며 그 효과는 첨가농도가 증가할수록 항산화효과도 크게 나타났다. 6$0^{\circ}C$에서 5일 동안 저장할 때, 산가의 경우RAE(상대적항산화효과)가 무첨가군을 100으로 보았을 때, 단삼의 경우 첨가량별로 130, 217, 269, 282로 증가하였다. POV의 RAE도 단삼의 경우 122, 187, 215, 267 증가를 보였다. TBA value의 RAE는 단삼은 137, 286, 419, 555로 뚜렷한 항산화 효과를 보였다. 따라서 단삼과 울금 추출물 첨가 약과가 무첨가군에 비하여 저장효과가 우수한 것으로 나타났다.

Keywords

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