Biomedical Science Letters (대한의생명과학회지)
- Volume 9 Issue 2
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- Pages.67-74
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- 2003
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Novel Trimeric Complex for Efficient Uptake of Plasmid Vector into HepG2 Cells
- Joo, Jong-Hyuck (Department of Medical Genetic Engineering, Keimyung University School of Medicine, Department of Biology, Chungnam National University) ;
- Park, Jong-Gu (Department of Medical Genetic Engineering, Keimyung University School of Medicine)
- Published : 2003.06.01
Abstract
Viral and non-viral vectors have been used in the delivery of genetic materials into animal cells and tissues, with each approach having pros and cons. Non-viral vectors have many useful merits such as easy preparation, low immunity and size tolerance of a transgene when compared to those of viral vectors. Delivery specificity may be achieved by complex formation between receptor ligands and a non-viral vector. In the present study, non-viral vector systems are investigated in an effort to find a practical delivery means for gene therapy, Receptor-ligand interaction between transferrin-receptor and transferrin was utilized for efficient gene transfer into cancer cells. A plasmid vector, pcDNA3 (LacZ) was ligated with a small duplexed oligo fragment in which a Biotin- VN