Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Evaluation of the Cytotoxicity of Calcium Phosphate Root Canal Sealers

인산 칼슘 근관 봉함재의 세포독성에 관한 연구

  • Lee, Jee-Hyun (Department of Conservative Dentistry, College of Dentistry, Seoul National University) ;
  • Baek, Seung-Ho (Department of Conservative Dentistry, College of Dentistry, Seoul National University) ;
  • Bae, Kwang-Shik (Department of Conservative Dentistry, College of Dentistry, Seoul National University)
  • 이지현 (서울대학교 대학원 치의학과 치과보존학교실) ;
  • 백승호 (서울대학교 대학원 치의학과 치과보존학교실) ;
  • 배광식 (서울대학교 대학원 치의학과 치과보존학교실)
  • Published : 2003.07.01
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Abstract

본 연구에서는 인산 칼슘 근관 봉함재 [Apatite Root Sealer (ARS) Type I, II, III]의 세포독성을 혼합후의 시간 경과에 따라서 다른 4계열의 근관 봉함재 (Pulp Canal Sealer EWT, AH Plus, Sealapex, Ketac Endo)와 비교하여 평가하였다. 근관 봉함재를 혼합한 후 1시간, 8시간, 24시간, 48시간, 1주, 2주,4주의 기간동안 배양액을 이용하여 추출액을 얻었다. L929 쥐섬유아세포를 24시간동안 각 시간군에서 얻은 추출액과 함께 배양한 후 dimethylthiazol diphenyltetrazolium (MTT) assay와 neutral red (NR) assay를 이용하여 세포독성(%)을 평가하였다. 실험 결과 ARS Type I, II, III는 전 시간군에 걸쳐 낮은 세포독성을 보였고 (23.65-0.55%) 특히 경화 초기에 다른 계열의 근관 봉함재보다 유의성 있게 낮은 세포독성을 나타내었다. ARS Type I, II, III간의 세포독성은 각 시간군에서 유의성 있는 차이를 보이지 않았다. AH Plus와 Ketac Endo는 초기에 높은 세포독성을 보였으나 AH Plus는 8시간 이후에, Ketac Endo는 24시간 이후에 독성이 급격히 감소하여 ARS와 유의성 있는 차이를 보이지 않았다. Pulp Canal Sealer EWT와 Sealapex는 4주까지 지속적인 세포독성을 나타내었다. 그리고 MTT assay와 NR assay를 이용하여 얻은 각 근관봉함재의 세포독성은 시간 경과에 따라서 비슷한 변화 양상을 나타내었다. 인산 칼슘 근관 봉함재는 생체적합성이 우수한 재료로써 앞으로 지속적인 개발과 평가가 필요할 것으로 사료된다.

Keywords

References

  1. Brown WE and Chow LC : A new calcium phosphate setting cement. J Dent Res 62:672, 1983
  2. Krell KF and Wefel JS : A calcium phosphate cement root canal sealer-scanning electron microscopic analysis. J Endodon 10:571-576,1984 https://doi.org/10.1016/S0099-2399(84)80103-X
  3. Sugawara A, Chow LC, Takagi S and Hanan C : In vitro evaluation of the sealing ability of a calcium phosphate cement when used as a root canal sealer-filler. J Endodon 16:162-165, 1990 https://doi.org/10.1016/S0099-2399(06)81963-1
  4. Cherng AM, Chow LC and Takagi S : In vitro evaluation of a calcium phosphate cement root canal filler/sealer. J Endodon 27:613-615, 2001 https://doi.org/10.1097/00004770-200110000-00003
  5. Chohayeb AA, Chow LC and Tsaknis PJ : Evaluation of calcium phosphate as a root canal sealer-filler material J Endodon 13:384-387,1987 https://doi.org/10.1016/S0099-2399(87)80198-X
  6. Chau JY, Hutter JW, Mork TO and Nicoll BK : An in vitro study of furcation perforation repair using calcium phosphate cement. J Endodon 23:588-592, 1997 https://doi.org/10.1016/S0099-2399(06)81129-5
  7. Goodell G, Mark TO, Hutter JW and Nicoll BK : Linear dye penetration of a calcium phosphate cement apical barrier. J Endodon 23:174-177, 1997 https://doi.org/10.1016/S0099-2399(97)80270-1
  8. Yoshimine Y and Maeda K : Histologic evaluation of tetracalcium phosphate-based cement as a direct pulpcapping agent. Oral Surg 79:351-358, 1995 https://doi.org/10.1016/S1079-2104(05)80229-X
  9. Bilginer S, Esener IT, Soylemezoglu F and Tiftik AM : The investigation of biocompatibility and apical microleakage of tricalcium phosphate based root canal sealers. J Endodon 23:105-109, 1997 https://doi.org/10.1016/S0099-2399(97)80255-5
  10. Yoshikawa M, Hayami S, Tsuji I and Toda T : Histopathological study of a newly developed root canal sealer containing tetracalcium-dicalcium phosphates and 1.0% chondroitin sulfate. J Endodon 23:162-165, 1997 https://doi.org/10.1016/S0099-2399(97)80267-1
  11. Telli C, Serper A, Kogan L and Guc D : Evaluation of the cytotoxicity of calcium phosphate root canal sealers by MTT assay. J Endodon 25:811-813, 1999 https://doi.org/10.1016/S0099-2399(99)80303-3
  12. Mosmann T : Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 65:55-63, 1983 https://doi.org/10.1016/0022-1759(83)90303-4
  13. Borenfreund E and Puerner JA : Toxicity determination in vitro by morphological alterations and neutral red absorption. Toxicol Lett 24:119-124, 1985 https://doi.org/10.1016/0378-4274(85)90046-3
  14. Hensten-pettersen A : Comparison of the methods available for assessing cytotoxicity. Int Endod J 21:89-99, 1988 https://doi.org/10.1111/j.1365-2591.1988.tb00973.x
  15. Schweikl H and Schmalz G : Toxicity parameters for cytotoxicity testing of dental materials in two different mammalian cell lines. Eur J Oral Sci 104:292-299, 1996 https://doi.org/10.1111/j.1600-0722.1996.tb00080.x
  16. Nakamura H, Sakakibara F, Matsumoto Y, Hirano S, Hayakawa H, Sakai K and Yip M : Study on the cytotoxicity of root canal filling materials. J Endodon 12: 156-160, 1986 https://doi.org/10.1016/S0099-2399(86)80054-1
  17. Azar NG, Heidari M, Bahrami Z and Shokri F : In vitro cytotoxicity of a new epoxy resin root canal sealer. J Endodon 26:462-465, 2000 https://doi.org/10.1097/00004770-200008000-00008
  18. Briseno BM and Willershausen B : Root canal sealer cytotoxicity on human gingival fibroblasts. I. Zincoxide-eugenol-based sealers. J Endodon 16:383-386, 1990 https://doi.org/10.1016/S0099-2399(06)81910-2
  19. Beltes P, Koulaouzidou E, Kotoula V and Kortsaris AH : In vitro evaluation of the cytotoxicity of calcium hydroxide-based root canal sealers. Endod Dent Traumatol 11:245-249, 1995 https://doi.org/10.1111/j.1600-9657.1995.tb00497.x
  20. Leonardo RT, Consolaro A, Iracilda ZC and Leonardo MR : Evaluation of cell culture cytotoxicity of five root canal sealers. J Endodon 26:328-330, 2000 https://doi.org/10.1097/00004770-200006000-00005
  21. Briseno BM and Willershausen B : Root canal sealer cytotoxicity on human gingival fibroblasts. III. Calcium hydroxide-based sealers. J Endodon 18:110-113, 1992 https://doi.org/10.1016/S0099-2399(06)81309-9
  22. Duarte MAH, Demarchi ACCO, Giaxa MH, Kuga MC, Fraga SC and Souza LCD : Evaluation of pH and calcium ion release of three root canal sealers. J Endodon 26:389-390, 2000 https://doi.org/10.1097/00004770-200007000-00002
  23. Leyhausen G, Heli J, Reifferscheid G, Waldmann P and Geurtsen W : Genotoxicity and cytotoxicity of the epoxy resin-based root canal sealer AH Plus. J Endodon 25:109-113, 1999 https://doi.org/10.1016/S0099-2399(99)80007-7
  24. Ersev H, Schmalz G, Bayirli G and Schweikl H : Cytotoxic and mutagenic potencies of various root canal filling materials in eukaryotic and prokaryotic cells in vitro. J Endodon 25:359-363, 1999 https://doi.org/10.1016/S0099-2399(06)81172-6
  25. Beltes P, Koulaouzidou E, Kolokuris I and Kortsaris AH : In vitro evaluation of the cytotoxicity of two glass-ionomer root canal sealers. J Endodon 23:572-574, 1997 https://doi.org/10.1016/S0099-2399(06)81124-6
  26. Mjor IA, Hensten-Pettersen A and Orstavik D. Biological properties. In: Mjor IA, ed. Dental materials: biological properties and clinical evaluation. Boca Raton, FL: CRC Press, Inc., 125-136, 1985
  27. Ishikawa K, Takagi S, Chow LC, Ishikawa Y, Eanes ED and Asaoka K : Behavior of a calcium phosphate cement in simulated blood plasma in vitro. J Dent Mater 10:26-32, 1994 https://doi.org/10.1016/0109-5641(94)90018-3

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