Fumigant and Repellency Effects of Terpenes against the Two-Spotted Spider Mite, Tetranychus urticae (Acari: Tetranychidae)

점박이응애에 대한 Terpene류의 훈증과 기피효과

  • 이소영 (충북대학교 농과대학 농생물학과) ;
  • 유정수 (충북대학교 농과대학 농생물학과) ;
  • 문선주 ((주)비아이지) ;
  • 이상길 (남부임업시험장) ;
  • 김철수 (임업연구원 산림병해충과) ;
  • 신상철 (임업연구원 산림해충과) ;
  • 김길하 (충북대학교 농과대학 농생물학과)
  • Published : 2003.09.01

Abstract

These studies were carried out to investigate fumigant, contact toxicity and repellency effects of 34 terpenes against acaricide susceptible the two-spotted spider mite, Tetranychus urticae. In addition, the efficacy was also tested against two acaricide-resistant strains. Two terpenes, isosafrole and safrole showed fumigant toxicity of 98.4%, at 10${\mu}\ell$/1 (air) concentration. LD$\_$50/ values of these two terpenes were 2.6${\mu}\ell$/1 and 4.3${\mu}\ell$l/1, respectively. Most terpenes showed low or no contact toxicity, except isosafrole showing 60.2% mortality against eggs. Hexanoic acid and limonene showed repellency effects of 79.1%, 87.8%, respectively, to the susceptible strain at concentration of 1,000 ppm in the lab conditions. Hexanoic acid (1,000 ppm) showed repellency effected of 77.8% and 83.3% to fenpropathrin and pyridaben resistant strains, respectively. However, limonene showed no repellency to the two resistant strains.

점박이응애(Tetranychus urticae)에 대한 34종 terpene의 훈증독성, 접촉독성 및 기피효과를 조사하였다. 훈증독성은 10${\mu}\ell$/1(공기)의 농도에서 isosafrole, safrole이 모두 98.4%의 살비율을 나타내었다. 이 두 화합물의 LD$_{50}$값은 각각 2.6, 4.3${\mu}\ell$/1이었다. 접촉독은 isosafrole만이 알에 대해서 60.2%의 살란활성을 보인 것 외에 대부분의 terpene에서 활성이 낮거나 없었다. 약제감수성 계통에 대한 기피반응을 조사한 결과 hexanoic acid와 limonene이 1,000ppm에서 각각 79.1%와 87.8%의 기피효과를 나타내었다. 한편 fenprothion저항성 계통과 pyridaben저항성계통에 대해서 hexanoic acid는 각각 77.8%과 83.3%의 기피효과를 나타내었다. 그러나 두저항성계통에 대한 limonene(1,000ppm)의 기피효과는 각각 17.8%와 10.0%로서 감수성계통에 대한 기피효과와 상반된 결과를 나타내었다.다.

Keywords

References

  1. Ahn, YJ., J.K. Yoo, J.R. Cho, J.O. Lee and S.c. Moon. 1996. Evaluation of effectiveness of AC 303630 and flucycloxuron mixtures against Tetranychus urticae (Acari: Tetranychidae) under laboratory and field conditions. Appl. Entomol. Zool. 31: 67-73
  2. Asda, M. 1978. Genetics and biochemicals mechanism of acaricide resistance in phytophagous mites. J. Pestic. Sci. 3: 61-68 https://doi.org/10.1584/jpestics.3.61
  3. Banthorpe, D.V. 1991. Classification of terpenoids and general procedures for their characterization. pp. 1-41. In B.V. Charlwood and D.V. Banthorpe [eds.], Methods in Plant Biochemistry. Vol. 7. Terpenoids. Academic, London
  4. Brattsten, L.B. 1983. Cytochrome P-450 involvement in the interactions between plant terpenes and insect herbivores. pp. 173-195. In P.A. Hedin [ed.], Plant Resistance to Insects, ACS (Am. Chem. Soc.), Washington, DC.
  5. Byers, J.A., Q.H. Zhang and G, Birgersson. 2000. Strategies of a bark beetle, Pityogenes bidentatus, in an olfactory landscape. Naturwissenschaften 87: 503-507 https://doi.org/10.1007/s001140050768
  6. Cho, J.R., Y.J. Kim, Y.J. Ahn , J.K. Yaa and J.O. Lee. 1995. Monitoring of acaricide resistance in field-collected papultions of Tetranychus urticae (Acari: Tetranychidae) in Korea. Korean J. Appl. Entomol. 34: 40-45
  7. Croft, B.A., S.C. Hoyt and P.H Westigard. 1987. Spider mite on fruits, revised: organotin and acaricide resistance management. J. Econ. Entomol. 80: 304-311
  8. Collart, M.G. and S.P. Hink. 1986. Sublethal effects of d-limonene on the cat flea (Ctenocephalides jelis), Entomol. Exp. Appl. 42: 225-229 https://doi.org/10.1007/BF00629308
  9. Dev, S., A.P.S. Narula and J.S. Yadav. 1982. Monoterpenoids, pp. 7-27. In S. DEV [ed.], CRC Handbook of Terpenoids. CRC. Boca Raton, FL
  10. Hink, W.F. and B.J. Fee. 1986. Toxicity of D-limonene, the major component of citrus peel oil, to all life stages of the cat flea, Ctenocephalides felis (Siphonaptera: Pulicidae). J. Med. Entomol. 23: 400-404
  11. Ho, C.C. 2000. Spider mite problems and control in Taiwan. Exp. Appl. Acarol. 24: 453-462 https://doi.org/10.1023/A:1006443619632
  12. Keita, S.M., C. Vincent, J.P. Schmit, S. Ramaswamy and A. Belanger. 2000. Effect of various essential oils on Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). J. Stored Prod. Res. 36: 355-364 https://doi.org/10.1016/S0022-474X(99)00055-7
  13. Kim, G.H., Y.H. Choi and K.Y. Cho. 1997. Behavioral responses of bifenthrin- and dicofol-resistant strains in the two-spotted spider mite, Tetranychus urticae Koch to bifenthrin. Korean J. Entomol. 27: 99-105
  14. Kim, Y,J., H.E. Lee, S.W. Lee, G.H. Kim and Y.J. Kim. 1999. Toxicity of tebufenpyrad to Tetranychus urticae (Acari: Tetranychidae) and Amblyseius womersleyi (Acari: Phytoseiidae) under laboratory and field conditions. J. Econ. Entomol. 92: 187-192
  15. Kim, D.H. and Y.J. Ahn. 2001. Contact and fumigant activities of consituents of Foreniculum vulgare fruit against three coleopteran stored-product insects. Pest Manag. Sci. 57: 301-306 https://doi.org/10.1002/ps.274
  16. Kim. E.H .. H.K. Kim and Y.J. Ahn. 2003. Acaricidal activity of plant essential oils against Tyrophagus putrescentiae (Acari: Acaridae). J. Asia-Pacific Entomol. 6: 77-82 https://doi.org/10.1016/S1226-8615(08)60171-5
  17. Kono, S. 1985. Susceptibility of dicofol resistant two-spotted spider mite, Tetranychus urticae Koch against various pesticides and their control effects. Jpn. J. Appl. Entomol. Zool. 29: 150-157 https://doi.org/10.1303/jjaez.29.150
  18. Lee, S.W. 1990. Studies on the pest status and integrated mite management in apple orchards. Ph.D. dissertion, Seoul National University, Suwon.
  19. Lee, S., R. Tsao, C. Peterson and J.R. Coats. 1997. Insecticidal activity of monoterpenoides to western corn rootworrn (Coleoptera: Chrysomelidae), twospotted spider mite (Acari: Tetranychidae), and house fly (Diptera: Muscidae). J. Econ. Entomol. 90: 883-892
  20. Lee, B.H., S.E. Lee, P.C. Annis, S.J. Pratt, B.S. Park and F. Tumaalii. 2002. Fumigant toxicity of essential oils and monoterpenes against the red flour beetle, Tribolium castaneum Herbst. J. Asia-Pacific Entomol. 5: 237-240 https://doi.org/10.1016/S1226-8615(08)60158-2
  21. Lee, Y.S., M.H. Song, K.S. Ahn, K.Y. Lee, J.W. Kim and G.H. Kim. 2003. Monitoring of acaricide resistance in two-spotted spider mite (Tetranychus urticae) populations from rose greenhouses in Korea. J. Asia-Pacific Entomol. 5: 237-240 https://doi.org/10.1016/S1226-8615(08)60158-2
  22. Martison, T.E., T.J. Dennehy, J.P. Nyprop and W.H. Reissing. 1991. Field measurements of selection for two-spotted spider mite (Acari: Tetranychidae) resistance to dicofol in apple orchards. J. Econ. Entomol. 84: 7-16
  23. Ngoh, S.P., L.E.W. Choo, F.Y. Pang, Y. Huang, M.R. Kini and S.H. Ho. 1998. Insecticidal and repellent properties of nine volatile constituents of essential oils against the American cockroach, Periplaneta americana (L.). Pestic. Sci. 54: 261-268 https://doi.org/10.1002/(SICI)1096-9063(1998110)54:3<261::AID-PS794>3.0.CO;2-C
  24. Rice, P.J. and J.R. Coats. 1994. Insecticidal properties of several monoterpenoids to the house fly (Diptera: Muscidae), red plour beetle (Coleoptera: Tenebrionidae), and southern corn rootworm (Coleoptera: Chrysomelidae). J. Econ. Entomol. 87: 1172-1179
  25. Roger, C.R. and A. Hamraoui. 1995. Fumigant toxic activity and reproductive inhibition induced by monoterpenes on Acanthoscelides obtectus (Say) (Coloeptera), a bruchid of kidney bean (Phaseolus vulgaris L.). J. Stored. Res. 31: 291-299 https://doi.org/10.1016/0022-474X(95)00025-3
  26. SAS Institute. 1991. SAS/STAT User's Guide: Ststistics, version 6.04. SAS Institute, Cary, N.C., U.S.A
  27. Song, C., G.H. Kim, S.J. Ahn, N.J. Park and K.Y. Cho. 1995. Acaricide susceptibilities of the field collected populations of two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae) from apple orchards. Korea J. Appl. Entomol. 34: 328-333
  28. Takafuji, A., A. Ozawa, H. Nemoto and T. Gotoh. 2000. Spider mites of Japan: their biology and control. Exp. Appl. Acarol. 24: 319-335 https://doi.org/10.1023/A:1006453209154
  29. Taylor, W.E. and B. Viekery. 1974. Insecticidal properties of limonene, a constituent of citrus oil. Ghana J. Agr, Sci. 7: 61-62
  30. Templetion, W. 1969. An introduction of the chemistry of terpenoids and steroids. Butterworths, London.
  31. Tiberi, R., A. Niccoli, M. Curini, F. Epifano, M.C. Marcotullio and O. Rosati. 1999. The role of the monoterpene composition in Pinus spp. needles, in host selection by the pine processionary caterpillar, Thaumetopoea pityocampa. Phytoparasitica 27: 263-272 https://doi.org/10.1007/BF02981482
  32. Yoo, J.S., G.H. Kim, S.G. Lee, S.C. Shin, J.D. Park and S.C. Park. 2002. Insecticidal activity and ovipositional repellency of monoterpenoids against Moechotypa diphysis Adults (Coleoptera: Cerambycidae). Korean J. Appl. Entomol. 41: 285-292
  33. Zar, J.H. 1996. Biostatical Analysis, 3rd ed. Prentice-Hall International, Inc