Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation of Bacteriophage from Haloarcular sp, EH-1

Haloarcular sp. EH-1에 의한 bacteriophage의 분리

  • 정명주 (경성대학교 교양과정부)
  • Published : 2003.08.01
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Abstract

The extremely halophilic archaebacteriurn Haloarcular sp. EH-1 was isolated from solar salts. Halophages found in Haloarcular sp. EH-1 were isolated from fermented anchovy sauce. Halophages were isolated from fermented anchovy sauce using Haloarcular sp. EH-1 as a host bacterium. The isolated halophage produced 0.5∼l.0 mm in diameter clear plaques. The halophage consists of an symmetrical head, measuring 68 nm in diameter, and a contractile tail, 100 nm long and base plates were observed. Total size of phage DNA genome obtained 20 Kbp and its sequence homology was 52.87% with H. Salinarium.

멸치 젓갈에서 분리된 파아지의 일반적인 성질과 파아지 DNA 서열을 분석한 결과는 다음과 같다. 멸치 젓갈에서 분리된 파아지의 플라그는 대체로 작고 선명한 핀 형태로 직경 0.5∼l.0 mm이었으며, 파아지의 형태는 지름 68 nm의 대칭형 두부와 길이 100 nm의 contractile 미부를 가지고 있었고 base plate가 관찰되었다. 파아지 DNA의 크기는 약 20 Kbp 정도이었으며, DNA 염기서열은 H. salinarium 파아지 hp 32와 52.87%의 상동성을 나타내었으나 보다 정확한 분류를 위해 파아지 DNA의 전체 염기서열에 대한 분석이 이루어져야 할 것이다.

Keywords

References

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