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Effects of Houttuynia Cordata thunb on the liver damage of TCDD-treated rats

TCDD를 투여한 rat의 간손상에 대한 어성초의 효과

  • 하배진 (신라대학교 공과대학 생명공학과) ;
  • 하종명 (신라대학교 공과대학 생명공학과) ;
  • 이상현 (신라대학교 공과대학 생명공학과) ;
  • 이재화 (신라대학교 공과대학 생명공학과) ;
  • 정혜진 (신라대학교 공과대학 생명공학과) ;
  • 이상헌 (신라대학교 공과대학 생명공학과) ;
  • 김희진 (신라대학교 공과대학 생명공학과) ;
  • 이진영 (인제대학교 의과대학 생화학 교실)
  • Published : 2003.08.01

Abstract

Houttuynia Cordata thunb has been used as folk medicine for analgesics, beriberi, edema, hepatitis and icterus etc. We investigated, the effects of Houttuynia Cordata thunb administration on protective in liver of 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD) treated rats. Seven days after the injection of TCDD(1${\mu}g$/kg), Houttuynia Cordata thunb (200mg/kg) was administered into rats intraperitoneally for four weeks. We examined the antioxidative enzymatic activity by measuring the level of GOT, GPT in serum and MDA, GSH, GSSG, GPx, SOD and Catalase in liver tissue of rats. GOT activity of Houttuynia Cordata thunb and TCDD administered group(HTT) showed 49.00% of inhibitive effect compared to TCDD-treated abnormal group(TTA). GPT level of HTT group was decreased to the level of Non TCDD-treated group(NTT). MDA content in the TTA group was 1.27 times increased compared to NTT group. HTT group was inhibited by 69.53% compared to TTA group. GSH contents in HTT group was 1.91 times increased compared to TTA group. GSSG contents in HTT group was 46.72% decreased compared to TTA group. SOD and Catalase in TTA group were lower than in NTT group, but SOD and Catalase in HTT group were increased by 82% and 55.45% respectively compared to TTA group.

TCDD를 투여한지 일주일 후부터 어성초를 200mg/kg 복강 내로 4주간 격일로 투여하였다. TTA군과 NTT군은 saline을 투여하였다. 혈청에서 GOT, GPT의 활성도를 측정하였고, 간조직중에서 MDA, GSH, GSSG, GPx, SOD, Catalase의 level을 측정하였다. GOT 활성도는 HTT군이 TTA군과 비교해서 49.00%의 억제 효과를 보였다. 반면 GPT활성도는 TTA(29.70)군에 비해 68.36%의 억제효과를 보였다. MDA는 TTA군이 NTT군 보다 높게 나왔으며, HTT군은 NTT군에 비교하여 30.47%의 억제율을 보였다. HTT군의 GSH는 TTA군에 비해 1.91배 증가하였고, GSSG는 TTA군에 비해 46.72% 감소하였다. SOD는 TTA군이 NTT군보다 낮게 나타났고, HTT는 TTA군에 비해 46%의 증가를 보였다. Catalase는 TTA군(1.12mU/mg protein)의 활성은 NTT(3.44mU/mg protein)군 보다 32.56% 감소하였다. HTT군의 간의 Catalase 활성도는 TTA 군과 비교하여 50.00% 증가 하였다. 이러한 결과에서 보듯이 TCDD로 인해 생성된 free radical은 항산화 효소에 의해서 감소되었으며, 그리고 어성초가 항산화 효소 대신에 노화를 저해하는 작용을 하는 것으로 사료 된다.

Keywords

References

  1. Aylac, G. 1985. The effect of chronic ethanol indigestion on hepatic lipid peroxide, glutathione, plutathione peroxidase and glutathione trnsferase in rat. Toxicol. 35, 71-78.
  2. Bae Jin Ha. 1987. A Study on the Antilipidperoxidative Effects of Brazilin and Hematoxylin(I), Kor. J. Food Hygiene. 2, 35-40.
  3. Brewster, D. W., Madhukar, B. V. and Matsumura, F. 1982. Influence of 2,3,7,8-TCDD on the protein composition of the plasma membrane of hepatic cells from the rat. Biochem. Biophys. Res. Common. 107, 68-74. https://doi.org/10.1016/0006-291X(82)91670-9
  4. Byard, J. L. 1987. the toxicological significance of 2,3,7,8-tetra-chlorodibenzo-p-dioxin and related compounds in human adipose tissue. J. Toxicol. Environ. Health. 22, 381-403. https://doi.org/10.1080/15287398709531081
  5. Crapo, H. C., McCord, M. J. and Fridovich, I. 1978. Preparation and assay of superoxide dismutase. 382-393, In Fleischer, S. and Packer, I. (eds)., Methods in enzymology, 52, Academic Press, New York.
  6. Gerig, J. B., Jones, G., Butler, W. H. and Barnes, J. H., 1973, Toxic effect 2,3,7,8-trtrachlorodibenzo-pdioxin. Food Cosmet Toxicol. 11, 585-595. https://doi.org/10.1016/S0015-6264(73)80329-3
  7. Hook, G. E., Haseman, J. K. and Lucier, G.W. 1975. Induction and suppression of hepatic and extrahepatic microsomal foreign-compound metabolizing enzyme systems by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Chem. Biol. Interact. 10, 199-214. https://doi.org/10.1016/0009-2797(75)90113-1
  8. Huff J., Lucier G. and Tritscher A. 1994. Carcinogenesis of TCDD : experimental, mechanistic, and epidemiologic evidence. Annu. Rev. Pharmacol. Toxicol. 34, 343-372. https://doi.org/10.1146/annurev.pa.34.040194.002015
  9. Hutzinger O, Choudhry GG., Chittim BG. and Johnston LE. 1985. Formation of polychlorinated dibenzofurans and dioxins during combustion, electrical equipment fires and PCB incineration. Environ. Health. Perspect. 60, 3-9. https://doi.org/10.2307/3429939
  10. Jones, D. P., Eklow, L., Thor, H. and Orrenius S. 1981. Metabolism of hydrogen peroxide in isolated hepatocytes relative contribution of catalase and glutathione peroxidase in decomposiotion of endogenously generated $H_2O_2$. Arch. Biochem. Biophys. 21, 505-516.
  11. Jung, D. S. 1992. Chemical Composition of Saururus Chinensis on fatty acid and amino acids. Cheju Univ. Jour. (Natural Sci), 35, 111.
  12. Lowry. O. H., Rosenbrough. N.J., Farr, A.S. and Randall, R. J. 1951. Protein Measurenment with folin phenol reagent, J. Biol. Chem. 193, 256.
  13. Meister, A. and Anderson, M.E. 1983. Glutathione. Annu. Rev. Biochem. 52, 711-716. https://doi.org/10.1146/annurev.bi.52.070183.003431
  14. Mukerjce D. 1998. Health impact of polychloinated di-benzo-p-dioxin: a critical review. J Air Waste Mang Assoc. 48, 157-165. https://doi.org/10.1080/10473289.1998.10463655
  15. Piot, H. C., Goldworthy, T., Campbell, H.A. and Poland, A. 1980. Quantitative evaluation of the promotion by 2,3,7,8-tetrachlorodibenzo-p-dioxin of hepatocarcinogenesis from diethylnitrosamine. Cancer Res. 40, 3616-3620.
  16. Roberts, V. A., Rosenbrough. N.J., Farr, A.S. and Randall, R.J. 1951. Protein Mearusement with folin phenol reagent. J. Biol. Chem. 193, 256-259.
  17. Rosen, D.R., etal. 1983. Mutations with familial amyotrophic lateral sclerosis. Naturer 362, 59-62. https://doi.org/10.1038/362059a0
  18. Tainer, J. A., Getzoff, E. D., Richardson, J. S., and Richardson, D. C. 1983. Structure and mechanism of Cu, Zn, superoxide dismutase. Nature. 306, 274-287. https://doi.org/10.1038/306274a0