Korean journal of food and cookery science (한국식품조리과학회지)

Korean Society of Food and Cookery Science (한국식품조리과학회)
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Isoflavone Contents, Antioxidative and Fibrinolytic Activities of Red Bean and Mung Bean

팥과 녹두의 이소플라빈 함량과 항산화 및 혈전용해 활성

  • Published : 2003.06.01
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Abstract

This study was conducted to investigate the isoflavone (daidzein and genistein) contents, and the antioxidative and fibrinolytic activities of red and mung beans. Daidzein was not found in either the red or mung beans. The genistein contents of the red and mung beans were 40.7 and 27.8 mg/kg, respectively. Both samples had very high electron donating abilities and SOD-like activities. Fibrinolytic activities were not detected in the crude mung bean extract, but fibrinolytic substances were purified or activated under various pH conditions and with heat treatments. With heat treatment at 100 $^{\circ}C$ for 10 min, the specific activity was increased 4.61 fold. This study revealed that, although red and mung beans were poor in isoflavone, they could be good sources for functional products due to their strong antioxidative activities and heat- and acid-resistant proteolytic abilities.

팥과 녹두는 떡의 소나 앙금 등 주로 전분의 특성을 이용하는 형태로 소비가 제한적인 편이다. 식생활을 통해 질병의 예방 및 개선 가능성이 대두되면서 각종 식물체로부터 여러 생리활성물질의 활성탐구 및 추출에 많은 노력이 이루어지고 있으나, 대두와 같은 두류로 분류기는 팥과 녹두의 경우 이들 생리활성물질에 대한 인구는 거의 없는 실정이다. 본 연구에서는 대두에 비해 상대적으로 연구가 미진한 팥과 녹두의 생리활성 및 이소플라본 함량을 조사하였고, 그 결과는 다음과 같다. 1.팥과 녹두에서는 daidzein이 검출되지 않았고, genistein은 건조중량 1 kg당 40.7 mg과 27.8 mg이 들어 있었다. 2. 팥과 녹두의 전자공여능은 90%의 활성을 보인 팥이 84.6%의 활성을 나타낸 녹두에 비해 유의적으로 높았다. 3. 팥과 녹두의 SOD 유사활성 역시 거의 같은 수준으로 높았고, 매우 안정적이었다. 4. 혈전용해능은 팥의 경우 원액에서는 활성이 없었으나, 추출액의 pH를 6.4로 조절한 후 18시간 동안 냉장처리한 결과 비교적 큰 활성을 보였으며, pH 3.0으로 조절시 더욱 증가하였다. 녹두는 추출액을 pH 6.4와 3.0으로 조절시 모두 비활성도가 증가하였으나 pH 3.0에서는 pH 6.4보다 활성이 낮았다. 이들 시료는 36$^{\circ}C$에서 배양시 14시간까지는 반응을 거의 보이지 않다가 15시간이 지나면서 활성이 크게 나타나는 특이성을 보였다. 5. 열처리가 혈전용해활성에 미치는 영향을 비교해보면, 팥과 녹두 모두 10$0^{\circ}C$에서 10분 처리시 가장 활성이 컸고, 그 다음이 55$^{\circ}C$에서 30분, 10$0^{\circ}C$에서 30분 순으로 유사한 양상을 보였다 특히 10$0^{\circ}C$에서 10분간의 열처리시 55$^{\circ}C$에서 30분 처리한 것에 비해 혈전용해활성이 증가한 것으로 미루어 팥과 녹두의 혈전용해활성은 열에 대한 안정성이 크다고 할 수 있다. 반면 10$0^{\circ}C$에서 30분간의 과도한 열처리는 활성의 저하를 초래했다.

Keywords

References

  1. http://www.nso.go.kr/report/data/svca9900.htm
  2. 한국영양학회:한국인의 영양권장량. 제 7차 개정. 중앙문화사, pp485-489, 2001
  3. Kwon, HJ : Bioactive compounds of soybean and their activity in angiogenesis regulation. Korean Soybean Digest, 16(1):63, 1999
  4. Sohn, HS, Lee, YS, Shin, HC and Chung HK : Does soybean isoflavone have adverse effects on human? Korean Soybean Digest, 17(2):9, 2000
  5. Knight, DC and Eden, JA : A review of the clinical effects of phytoestrogens. Obstet. Gynecol., 87(5):897, 1996
  6. Cassidy, A : Physiological effects of phyto-estrogens in relation cancer and other human health risks. Proc. Nutr. Soc., 55:399, 1996 https://doi.org/10.1079/PNS19960038
  7. Choi, YB and Sohn, HS : Isoflavone content in Korean fermented and unfermented soybean foods. Korean J. Food Sci. Technol., 30(4):745, 1998
  8. Kim, JS and Kwon, TW : Estimated dietary isoflavone intake of Korean population based on national nutrition survey. Nutrition Research, 21:947, 2001 https://doi.org/10.1016/S0271-5317(01)00310-4
  9. Wakai, K, Egami, I, Kato, K, Kawamura, T, Tamakoshi, A, Lin, Y, Nakayama, T, Wada, M and Ohno, T :Dietary intake and sources of isoflavones among Japanese. Nutrition and Cancer, 33(2):139, 1999 https://doi.org/10.1207/S15327914NC330204
  10. Voet, D and Voet, JG : Biochemistry. p. 1087, John Wileysons, New York, , 1990
  11. Kim, JH : Purification of a plasminogen activator from the Scolopendra. Theses collection of Sangji university, 15:1, 1994
  12. Mihara, H, Nakajima, N and Sumi, H : Characterization of potent fibrinolytic enzymes in earthworm, Lumbricus rubellus. Biosci. Biotech. Biochem., 57(10):1730, 1993
  13. Kim, YT, Kim, WK and Oh, HS : Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkookjang. Appl. Environ. Microbiol., 62:2482, 1996
  14. Kim, SH : New trends of studying on potential activities of Doen-jang - fibrinolytic activity -. Korean Soybean Digest, 15(1):8, 1998
  15. Sumi, H, Hamada, H, Tsushima, H, Mihara, H and Muraki, HA : A noble fibrinolytic enzyme(nattokinase) in the vegetable cheese Natto : a typical and popular soybean food in the Japanese diet. Experientia, 43:1110, 1987 https://doi.org/10.1007/BF01956052
  16. Jun, TH, Nam, MH, Lee, SK and Park, WC : Changes in peroxidase activity and its isozymes of sobean, red-bean and mung-bean during germination. J. Korean Agricultural Chemical Society, 26(3):151, 1983
  17. Lee, SK, Park, WC and Hong, JU : Isolation and characterization of two isoperoxidases from mung bean seedling, J. Korean Agri. Chem. Soc., 29(3):279, 1986
  18. Rho, JH and Rhee, HS : Physicochemical properties and gel-forming properties of corn and red bean crude starches. Korean J. Soc. Food Sci., 4(1):1, 1988
  19. Kim, AK, Kim, SK and Lee, AR : Comparison of chemical composition and gelatinization property of mungbean flour and starch. Korean J. Soc. Food Sci., 11(5):472, 1995
  20. Min, SH and Sohn, KH : A study of the foaming properties of mungbean protein isolate. Korean J. Soc. Food Sci., 4(2):1, 1990
  21. Kim, HJ, Sohn, KH and Park, HK : Emulsion properties of small red bean protein isolates. Korean J. Soc. Food Sci., 6(4):9, 1990
  22. Jeune, KH, An, MG, Jung, SN, Choi, KM, Lee, SH and Chung, SR : Effect of mung bean lectin(MBL) on cytokine gene expression from human peripheral blood mononuclear cells. Kor. J. Pharmacogn., 30(4):355, 1999
  23. Jeong, SJ, Kang, TH, Ko, EB and Kim, YC : Flavonoids from the seeds of Phaselous radiatus. Kor. J. Pharmacogn., 29(4):357, 1998
  24. Chang, YS and Mo, SM : Effects of germination on antinutritional oligosaccharieds of mung beans. J. Korean Soc. Food Nutr., 14(3):284, 1985
  25. Choi, KS : A study of elucidation of protein quality of raw and heated legumes fed by three different dietary levels on rats, J. Korean Home Economics Assoc 20(4):91, 1982
  26. Franke, AA, Custer, LJ, Cerna, CM and Narala, KK : Rapid HPLC analysis of dietary phytoesterogens from legumes and from human urine. Proc. Soc. Exp. Biol.Med., 208(1):18, 1995 https://doi.org/10.3181/00379727-208-43826
  27. Kim, SJ, Han, DS, Park, MH and Rhee, JS : Screening for superoxide dismutase - like compounds and its activators in extracts of fruits and vegetables. Biosci.Biotech. Biochem., 58(12):2263, 1994 https://doi.org/10.1271/bbb.58.2263
  28. Blois, MS : Antioxidant determinations by the use of a stable free radical. Nature, 181:1199, 1958 https://doi.org/10.1038/1811199a0
  29. Kim, YJ, Kim, CK and Kwon, YJ : Isolation of antioxidative components of Perillae semen. Korean J.Food Sci. Technol., 29(1):38, 1997
  30. Haverkate, F and Traas, DW : Dose-response curves in the fibrin plate assay. Fibrinolytic activity of protease. Thromb. Haemost., 32:356, 1974
  31. Lowry, OH, Rosenbrough, NJ and Randall, AJ : Protein measurement with the folin phenol reagent. J. Biol. Chern., 193:265, 1951
  32. Moon, BK, Jeon, KS and Hwang, IK : Isoflavone contents in some varieties of soybean and on processing conditions. Korean J. Soc. Food Sci., 12(4):527, 1996
  33. Choi, JS, Kwon, TW and Kim, JS : Isoflavone contents in same varieties of soybean. Foods and Biotechnology, 5(2):137, 1996
  34. Lee, MH, Park, YH, Oh, HS and Kwak, TS : Isoflavone content in soybean and its processed products. Korean J. Food Sci. Technol., 34(3):365, 2002
  35. Wang. H and Murphy, PA : Isoflavone composition of American and Japanese soybeans in Iowa: Effects of variety, crop year, and location. J. Agric. Food Chem.,42:1674, 1994 https://doi.org/10.1021/jf00044a017
  36. Kim, JS and Yoon, S. : Isoflavone contents and ($\beta$-glucosidase activities of soybeans, Meju, and Doenjang. Korean J. Food Sci. Technol., 31(6):1405, 1999
  37. Hui, HJ : Encyclopedia of Food Science and Technology. vol. 4 p239. In: Soybeans. A Wiley-Interscience Publication. New York. U.S.A., 1992
  38. Oh, HS, Kim, JH and Park, YH : Isoflavone contents, antioxidative and fitrinolytic activities of some commercial cooking-with rice soybeans. Korean J. Food Sci. Technol., 34(3):498, 2002
  39. Kim, SR, Hong, HD and Kim, SS : Some properties and contents of isoflavone in soybean foods. Korean Soybean Digest, 16(2):35, 1999
  40. Bae, EA, Kwon, TW and Moon GS : Isoflavone contents and antioxidative effects of soybeans, soybean curd and their by-products. J. Korean Soc. Food Nutr., 26(3):371, 1997
  41. Rhee, KS, Ziprin, YA and Rhee, KC : Antioxidant activity of methanolic extracts of various oilseed protein ingredients. J. Food Sci., 46:75, 1981 https://doi.org/10.1111/j.1365-2621.1981.tb14534.x
  42. Seo, TH, Kim, IJ, YIE, AS and Min HK : Electron donating ability and contents of phenolic compounds, tocopherols and carotenoids in waxy com(Zea mays L). Korean J. Food Sci. Technol., 31(3):581, 1999
  43. Choi, HC and Oh, SK : Diversity and function of pigments in colored rice(in Korea). Korean J. Crop Sci.. 41(Special Issue): 1, 1996
  44. Park, YJ, Kang, MH, Kim, JI, Park, OJ, Lee, MS and Jang, HD : Changes of vitamin C and superoxide dismutase(SOD)-like activity of persimmon leaf tea by processing method and extraction condition. Korean J. Food Sci. Technol., 27(3):281, 1995
  45. Morita, S, Fukase, M, Hoshino, K, Fukuda, Y, Yamaguchi. M and Morita, Y : Partial purification and characterization of a novel soybean protease which is inhibited by Kunitz and Bowman-Birk trypsin inhibitors. J. Biochem., 119(4):711, 1996 https://doi.org/10.1093/oxfordjournals.jbchem.a021300
  46. Kang, MH, Kim, YH and Lee, SR : Trypsin inhibitor and hemagglutinating activities of some minor beans in Korea. Korean J. Food Sci. Technol., 12(1):24, 1980
  47. Kembhavi, AA, Buttle, DJ, Knight, CG and Barrett, AJ : The two cysteine endopeptidases of legume seeds: Purification and characterization by use of specific fluorometric assay. Arch. of Biochem. and Biophy., 303(2):208, 1993 https://doi.org/10.1006/abbi.1993.1274