Effect of UV-B Irradiation on the content of vitamin D$_2$, color and flavor pattern in Lentinus edodes

자외선 B파 조사가 표고버섯의 비타민 D$_2$ 함량, 색도 및 향기패턴에 미치는 영향

  • 이진실 (상명대학교 외식영양학 전공) ;
  • 윤갑희 (임업연구원 화학미생물과) ;
  • 신원선 (한국식품개발연구원 식품안전연구실)
  • Published : 2003.02.01

Abstract

Lentinus ededes were irradiated with Utraviolet-B(UV-B) at 0, 2.5, 5.0 J/$\textrm{cm}^2$ levels while culturing in sawdust medium, and the changes in vitamin D$_2$ content, color and flavor of the mushroom were analysed by high-performance liquid chromatography (HPLC), Hunter-Lab Chromatometer and Gas Chromatography-Surface Acoustic Wave (GC-SAW) electric nose, respectively. Irradiation of 0, 2.5, 5.0 J/$\textrm{cm}^2$ doses of UV-B increased the content of vitamin D$_2$ in the mushroom significantly, which was 157∼206% higher than the control group. There was a significant difference in L value between the control group and UV-B irradiated (2.5 and 5.0 J/$\textrm{cm}^2$) groups. The changes of flavor pattern were detected by GC-SAW electric nose. But the color and flavor changes were not detrimental to the quality of the mushroom. The results suggested that UV-B irradiation is a good way to increase the vitamin D$_2$ content of Lentinus edodes during cultivation.

자외선 B파 조사가 톱밥배지에서 재배되고 있는 표고버섯의 비타민, 색도, 향기패턴에 미치는 영향에 대해 조사하였으며 그 결과는 다음과 같다. 1. 자외선 B파 조사에 따른 표고버섯의 비타민 D$_2$ 함량 변화. 자외선 B파를 자실체에 각각 0(대조군), 25, 50KJ/$m^2$ 조사한 결과 대조군의 비타민 D$_2$ 함량은 25.36$\mu\textrm{g}$/g인 반면, 25, 50KJ/$m^2$의 자외선 조사로 비타민 D$_2$ 함량은 각각 39.73, 52.35$\mu\textrm{g}$/g로 증가하였다. 2. 자외선 B파 조사량에 따른 색도 변화. 색도 측정 결과 명도를 나타내는 L$^{*}$ 값은 대조군이 94.84로 25(92.19), 50KJ/$m^2$(92.26)보다 높은 것으로 나타났으며 적색을 나타내는 a$^{*}$ 값은 각 시험군 간에 차이는 없었으나 황색을 나타내는 b$^{*}$ 값은 대조군이 -1.35로 25(-1.06), 50KJ/$m^2$(-0.80)보다 낮은 치수를 보였다. 3. 자외선 B파 조사량에 따른 표고버섯의 향기패턴 분석. GC-SAW 전자코를 통해 분석된 향기 패턴은 대조군의 경우 peak가 10개가 감지되었으나 25, 50KJ/$m^2$군의 경우는 14개의 peak들이 감지되었다. 자외선 조사로 표고버섯의 향기 패턴의 변화가 생긴 것을 알 수 있었다.

Keywords

References

  1. 김병각, 김양섭, 석순자, 성재모, 신재용, 안영남, 한정혜 : 버섯 건강요법. 가림출판사, 49, 1995
  2. Chang, ST and Buswell, JA : Mushroom nutriceuticals. World J. Microbiol. Bioiechnol., 12(5):473, 1996 https://doi.org/10.1007/BF00419460
  3. Kawamura, Y, Manabe, M, Kitt, K : Antitumor protein (AP) from a mushroom induced apoptosis to transformed human keratinocyte by controlling the status of pRb, c-MYC, cyclin E-cdk2 and p21 super(WAF1) in the G1/s transition. Bio Factors 12(1):157, 2000
  4. Takehara, M, Kuida, K and Mori, K : Antiviral activity of virus like particles from Lentinus edodes (Shiitake). Arch. Virol., 59(3):269, 1979 https://doi.org/10.1007/BF01317423
  5. Borchers, AT, Stern, JS;, Hackman, RM, Keen, CL, and Gershwin, M : Minireview: Mushrooms, Tumors, and Immunity. P.S.E.B.M, 221:281, 1999
  6. Ahn, DK : Medicinal Fungi in Korea. K. J. Mycology. 20(2): 154, 1992
  7. Jung, IC, Park, S, Park, KS, Ha, HC, Kim, SH, Kwon, YI., and Lee, JS : Antioxidantive effect of fruit body and mycelial extracts of Pleurotus ostreatus. Korean J. Food Sci. Technol. 28(3):464, 1996
  8. Ma, SJ : Effects of the Substances Extracted from Dried Mushroom(Lentinus edodes) by Several Organic Solvents on the Stability of Fat). Korean J. Food Sci. Technol., 15(2):72, 1983
  9. Friedeich W : Vitamins 143 de Gruyter, 1988
  10. Fraser DR : Vitamin D. Lancet 345(14): 104, 1995 https://doi.org/10.1016/S0140-6736(95)90067-5
  11. Collins DE and Normar AW : Vitamin D in Handbook of Vitamins edited by Machlin Lawrence J., 2nd ed. 59, 1991
  12. World Health Organization : Environmental Health Criteria 14: Ultraviolet Radiation, 17, 1979
  13. Dannenberg, MJ, Holick, MF, Hollis, BW, Lu, Z and Wortsman, J : Clothing prevents Ultraviolet-B Radiation-Dependent Photosynthesis of Vitamin D3, Journal of Clinical Endocrinology and Metabolism, 75(4): 1099, 1991
  14. Holick, MF, Matsuoka, LY, Wortsman, JN, Hanifan : Chronic sunscreen use decreases circulating concentrations of 25-hydroxy vitamin D, Arch Dermatol, 124:1802, 1988 https://doi.org/10.1001/archderm.124.12.1802
  15. Schaller, E, Bosset, JO, and Escher, F : 'Electronic Noses' and their application to food. Lebensm-Wiss. Technol., 31(4):305, 1998 https://doi.org/10.1006/fstl.1998.0376
  16. 노봉수, 오세연 : GC/SAW를 바탕으로한 전자코 응용. 식품과 산업, 35(3):50, 2002
  17. Lee, MS and Chung, MS : Analysis of flavor patttern by using electric nose and sensory evaluation of Cnidium Officinale-flavored oils. Korean J. Soc. Food Coolery Sci., 18(4):448, 2002
  18. Chung, MS and Lee, MS : Development of Elsholtzia splendens-flavored oils and analysis of flavor pattern using electric nose. Korean J. Soc. Food Cookery Sci., 18(4):455, 2002
  19. Mattila, PH, Piironen, VI, Unsi-Rauva, EJ, and Koivistoinen, PE : Vitamin D Contents in Edible Mushrooms. J. Agric. Food Chem. 42(11):2449, 1994 https://doi.org/10.1021/jf00047a016
  20. Scheunert, A, Reschke, J, and Schieblich, M : About the vitamin D contents in some edible mushrooms. Hoppe Seyler's Z. Physiol. Chem. 235:91, 1935 https://doi.org/10.1515/bchm2.1935.235.3-4.91
  21. Takamura, K, Hoshino, H, Sugahara, T, and Amano, H : Determination of vitamin $D_{2}$ in Shiitake mushroom by high performance liquid chromatography. J. Chromatogr 545(20):2041, 1991
  22. Takeuchi, A, Okano, T, Teraoka, S, and Murakami, T : High-performance liquid chromatographic determination of vitamin D in foods, feeds and pharmaceuticals by successive use of reversed-phase and straight-phase columns. J. Nutr. Sci. Vitaminol. 30(11):25, 1984
  23. Lee, JS, Ahn, RM and Choi, HS : Determinations of ergocalciferol and cholecalciferol in mushrooms. Korean J. Soc. Food Sci, 13(2): 173, 1997
  24. Lee, JS, Kim, SJ, Ahn, RM, Choi, HS, Choi, HK, Yoon, SK, Hong, WS, Whang, HS, Kwon, DJ, and Kim, YJ : The effect of UV-B irradiation and hot-air drying on the vitamin $D_{2}$ content of Shiitake mushroom (Lentinus edodes). Korean J. Soc. Food Sci, 18(2): 173, 2002
  25. Mau, JL, Chen, PR, and Yang, JH : Ultraviolet irradiation increased vitamin $D_{2}$ content in edible mushrooms, J. Agric. Food Chem. 46(12):5269, 1998 https://doi.org/10.1021/jf980602q
  26. Ono, R, Arimoto, K, Kano, K, Matsuoka, K, Sugiura, W, Sadonte, H, and Mori, K : Vitamin $D_{2}$ formation in Lentinus edodes(Sfhiitake) by irradiation with fluorescent sunlamp. Mushroom Science IX (Part I), Proceeding, 1974
  27. Brubacher, GW and Southgate DAT : Methods for the determination of vitamins in food. Elsevier. p23, 97, 1991