Changes in Carotenoids Contents in Pureed and Cooked Carrot and Spinach during Storage

가열처리 및 저장조건에 따른 당근과 시금치퓨레의 Carotenoids 함량변화와 이성질화 형성에 관한 연구

  • ;
  • ;
  • Robert M, Russell (Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University)
  • 김혜영 (성신여자대학교 식품영양학과) ;
  • 임양이 (성신여자대학교 식품영양학과) ;
  • Published : 2003.02.01

Abstract

Investigations were conducted on the changes in carotenoids content, and quantification of cis-trans-${\beta}$-carotene Isomers in pureed and cooked carrot and spinach during storage. The isomerization and degradation of carotenoids were monitored by high-performance liquid chromatography on a C$\_$30/ reversed-phase column with diode-array detection. The results showed that lutein, ail-trans-${\beta}$-carotene, ${\alpha}$-carotene, 9-cis-${\beta}$-carotene and 13-cis-${\beta}$-carotene were present in carrot and spinach. Zeaxanthin and cryptoxanthin were present in raw spinach. The contents of lutein, zeaxanthin, cryptoxanthin, ${\alpha}$-carotene and all-trans-${\beta}$-carotene in pureed and cooked carrot and spinach decreased with increasing storage period. The 9-cis and 13-cis carotenoid isomers were the major types formed in cooked carrot during storage. Cooking was not found to alter the carotenoid profile of the sample, but increased the total amount of carotenoids compared with pured ones. This increase could be explained that cooking itself increased the extraction efficiency and inactivated the enzymes degradating carotenoids.

Keywords

References

  1. Pfander, H : Key to Carotenoids, 2nd ed., Birkhauser Verlag, Basel, Switzland., 1987
  2. Olson, JA : Biological actions of carotenoids, J. Nutr., 119(1):94, 1989
  3. Quackerbush, FW : Reversed-phase HPLC separation of cis and trans-carotenoids and its application to beta carotene in food materials. J. Liquid Chrom., 10:643, 1987 https://doi.org/10.1080/01483918708069015
  4. Erdman, JW, Jr., Poor CL and Dietz, JM : Factors affecting the bioavaility of vitamin A, carotenoids, and vitamin E. Food Technol., Oct., 42(10)214, 1988
  5. Simpson, KL : Chemical changes in natuial food pigments. In: Chemical Changes in Food during Processing, AVI, Richardson T & Finley JW, eds., Westport, CT, P411, 1985
  6. Sweeney, JP and Marsh, AC : Effect of processing on provitamin A in vegetables. J. Am. Dietet. Assoc., 59:238, 1971
  7. Chen, BH : Studies on the stability of carotenoids in garland chrysanthemum(Ipomoea spp.) as affected by microwave and conventional heating. J. Food Prot., 55(4):296, 1992
  8. Chen, BH and Han, LH : Effects of different cooking methods on the yield of carotenoids in water convolvulus(Ipomoea aquatica). J. Food Prot., 53(12): 1076, 1990
  9. Khachik, F, Goli, MB, Beecher, GR, Holden, Lusby, WR, Tenorio, MD and Barrera, MR : Effect of food preparation on qualitative and quantitative distribution of major carotenoid contituents of yomatoes and several green vegetables. J. Agric. Food Chem., 40:390, 1992 https://doi.org/10.1021/jf00015a006
  10. Steinmetz, KA and Potter, JD : Vegetables, fruit, and cancer. II. Mechanism. Cancer Causes and Control., 2:427, 1991 https://doi.org/10.1007/BF00054304
  11. Chen, BH and Chen, YY : Stability of chlorophylls and carotenoids in sweet potato leaves during microwave cooking. J. Argric. Food Chem., 41:1315, 1993 https://doi.org/10.1021/jf00032a029
  12. Chandler, LA and Schwartz, SJ : Isomerization and losses of trans-$\beta$-carotene in sweet potatoes as affected by processing treatments. J. Agric. Food Chem., 36:129, 1988 https://doi.org/10.1021/jf00079a033
  13. Baloch, AK, Buckle, KA and Edwards, RA : Effect of processing on the quality of dehydrated carrot. J. Food Technol., 12:285, 1977 https://doi.org/10.1111/j.1365-2621.1977.tb00109.x
  14. Dietz, JM and Gould, WA : Effects of process stage and storage on retention of beta-carotene in tomato juice. J. Food Sci., 51(3):847, 1986 https://doi.org/10.1111/j.1365-2621.1986.tb13951.x
  15. Lee, CY, Massry Jr, LM and van Buren, JP : Effects of posthavest handling and processing on vitamin content of peas. J. Food Sci., 47(3):961, 1982 https://doi.org/10.1111/j.1365-2621.1982.tb12755.x
  16. Chandle, LA and Schwartz, SJ : HPLC seperation of cis-trans carotene isomers in fresh and processed fruits and vegetables. J. Food Sci., 52(3):669, 1987 https://doi.org/10.1111/j.1365-2621.1987.tb06700.x
  17. Dudek, JA, Elkins, ER, Chin, HB and Hagen, RE : Investigations to determine nutrient content of selectedfruits and vegetables-raw, Processed and prepared. Nat. Food Proc. Assoc. USDA Contract No. 43-32U4-8-92
  18. Edwards, CG and Lee, CY : Measuerment of provitarnin A carotenoids in fresh and canned carrots and green vegetables. J. Food Sci., 51(2):534, 1986 https://doi.org/10.1111/j.1365-2621.1986.tb11180.x
  19. Ogunlesi, AT and Lee, CY : Effect of thennal processing on the steroisomerization of major carotenoids and vitamin A value of carrots. Food Chem., 4(4):311, 1979 https://doi.org/10.1016/0308-8146(79)90019-0
  20. Gomez, MI : Carotene content of some green leafy vetables of Kenya and effects of dehyfration and storageon carotene retention. J. Plant Foods, 3:231, 1981
  21. Granado, F, Olmedilla, B, Blanco, I and Rojas-Hidalgo, E : Carotenoid composition in raw and cooked spanish vegetables. J. Agric. Food Chem., 40:2135, 1992 https://doi.org/10.1021/jf00023a019
  22. Panalaks, T and Murray, TK : Effect of processing on the content of carotene isomers in vegetables and peachs. J. Inst. Can. Technol., 3:145, 1970
  23. Booth, VH : Inactivation of the carotene-oxidizing system in Iris leaf. J. Sci. Food Agric., 11:8, 1960 https://doi.org/10.1002/jsfa.2740110102
  24. Ueno, T, Maekawa, A and Suzuki, T : Effect of lipoxygenase on the determination of carotene in vegetables. Vitamins(Jpn.), 56:83, 1982
  25. Pesek. CA and Warthesen, JJ : Kinetic model for photoisomerization and concomitant photodegradation of beta-carotenes. J. Agric. Food Chem., 38:1313, 1990 https://doi.org/10.1021/jf00096a004
  26. Marin, MA, Cano, P and Fuster, C : Freezing preservation of 4 spanish mango cu1tivars(Manigifera-indica L)-chemical and biochemical aspects. Zeitschriftfur Lebensmittel Untersuchung und Forschung, 194:566, 1992 https://doi.org/10.1007/BF01185485
  27. Yeum, KJ, Booth, SL, Sadowski, JA, Liu, C, Tang, G, Krinsky, NI. and Russell, RM : Human Plasma carotenoid response to the ingestion of controlled diets high in fruits and vegetables. Am. J. Clin. Nutr., 64:594, 1996
  28. Lessin, WJ, Catigani, GL and Schwartz, SJ : Quantification of cis-trans Isomers of provitamin A carotenoids in fresh and processed fruits and vegetables. J. Agric. Food Chem., 45:3728, 1997 https://doi.org/10.1021/jf960803z
  29. Chen, HE, Peng, HY and Chen, BH : Stability of carotenoids and vitamin A during storage of carrot juice. Food Chem., 57(4):497, 1996 https://doi.org/10.1016/S0308-8146(96)00008-8
  30. O'Neil, CA and Schwartz SJ : Photoisomerization of $\beta$-carotene by photosensitization with chlorophyll derivatives as sensitizer. J. Chromatogr., 624:235, 1992 https://doi.org/10.1016/0021-9673(92)85682-J
  31. Khachik, F, Goli, MB, Beecher, GR, Holden, J, Lusby, WR, Tenorio, MD and Barrera, MR : Effect of food preparation on qualitative and quantitative distribution of major carotenoid constituents of tomatoes and several green. J. Agric. Food Chem., 40:390, 1992 https://doi.org/10.1021/jf00015a006