Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Gentiobiose Synthesis from Glucose Using Recombinant $\beta$-Glucosidase from Thermus caldophilus GK24

  • Published : 2003.06.01
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Abstract

Recombinant $\beta$-glucosidase from Thermus caldophilus GK24 was easily purified partially by a heat treatment procedure, resulting in 8-fold and recovery yield of 80% from crude enzyme. When the $\beta$-glucosidase was incubated with a 80% glucose solution (w/w), gentiobiose ($\beta$1,6-glucobiose) was the major product in the reaction mixture. The optimal conditions for producing gentiobiose (11% yields of total sugar) were pH 8-9 and 7$0^{\circ}C$ for 72 h.

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References

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