Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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The Recommended Approaches for the Evaluation of Testicular Toxicity with Awareness of the Spermatogenic Cycle and Quantitative Testicular Toxicity Evaluation Methods

정자생성 주기법을 이용한 고환독성 평가 필요성과 정량적인 고환독성 평가방법에 대한 고찰

  • 손우찬 ;
  • 김종춘 (전남대학교 수의과대학 약리독성학교실) ;
  • 유일재 (산업안전공단 산업안전보건연구원 산업화학물질연구센터)
  • Published : 2003.06.01
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Abstract

Since histopathological examination was known to be the most sensitive evaluation for testicular toxicity, regulatory authorities have been published the guidelines on practical testicular assay approach. Those guidelines specified details of evaluation including fixation, embedding, stain-ing, histological examination and also seminiferous tubular staging methods. However, there have been confusing understanding among toxicologists and even pathologists on staging theory and its application on industrial testicular toxicity. Guidelines did not intend to conduct quantitative assay with staging but recommended the use of knowledge of staging. To count each tubular stage with statistical analysis is known to be time consuming and labor burdening work but the significance of toxicity has little value. It also has been pointed out that the application of staging theory for longer-term toxicity considered to be lacking of rationale. It could be recommended that qualitative assay with aware-ness of germ cell loss is more efficient method rather than quantitative counting of each tubular stage. Therefore it would be required that comprehensive understanding of testicular toxicity evaluation and the use of testicular staging method.

Keywords

References

  1. N. Engl. J. Med. v.332 Decline in semen quality among fertile men in Paris during the past 20 years Auger,J.;Kunstmann,J.M.;Czyglik,F.;Jouannet,P. https://doi.org/10.1056/NEJM199502023320501
  2. Fertil Steril v.67 A meta-analysis of 61 sperm count studies revisited Becker,S.;Berhane,K. https://doi.org/10.1016/S0015-0282(97)81446-X
  3. Fund. Appl. Toxicol. v.12 Optimal replication for histometric analysis of testicular function in rats and rabbits Berndtson,W.E.;Neefus,C.;Foote,R.H.;Amann,R.P. https://doi.org/10.1016/0272-0590(89)90046-8
  4. Fund. Appl. Toxicol. v.5 Potential indicators of reproductive toxicity: testicular sperm production and epididymal sperm number, transit time and motility in F344 rats Blazac,W.F.;Ernst,T.L.;Stewart,B.E. https://doi.org/10.1016/0272-0590(85)90145-9
  5. Br. Med. J. v.305 Evidence for decreasing quality of semen during past 50 years Carlsen,E.;Giwercman,A.;Keiding,N.;Skakkebaek.N.E. https://doi.org/10.1136/bmj.305.6854.609
  6. Am. J. Vet. Res. v.31 Testicular changes as affected by mating systems in beet cattle Carroll,E.J.;Ball,L.
  7. Reprod. Toxicol. v.12 Reproductive endpoints in general toxicity studies: are they predictive Chapi,R.E.;Sloane,R.A.;Hasemanm,J.K. https://doi.org/10.1016/S0890-6238(98)00026-4
  8. Toxicol. Pathol. v.17 Mechanistic approaches in the study of testicular toxicity: Toxicants that affects the endocrine regulation on the testis Chapin,R.E.;William,J. https://doi.org/10.1177/019262338901700220
  9. Male reproductive toxicology Chapin,R.E.;Hendel,J.J.
  10. Toxicol. Pathol. v.25 Evoluation of testicualr toxicity in safety evaluation studies: the appropriate use of spermatogenic staging Creasy,D.M. https://doi.org/10.1177/019262339702500201
  11. J. Dairy. Sci. v.43 Spermatogenesis in relation to spermatozoa concentration in bovine semen Cupps,P.T.;Laben,R.C. https://doi.org/10.3168/jds.S0022-0302(60)90239-3
  12. Fund. Appl. Toxicol. v.6 Use of quantitative testicular histopathology to assess the effects of DBCP on reproduction in rabbits Foote.R.H.;Berndtson,W.E.;Rounsavile,T.R. https://doi.org/10.1016/0272-0590(86)90176-4
  13. J. Toxicol. Sci., Spec No:233-9 Collaborative work to evaluate toxicity on male reproductive organs by repeated dose studies in rats 24. Testicular toxicity of boric acid after 2-and 4-week administration periods Fukuda,R.;Hirode,M.;Mori,I.;Chatani,F.;Morishima,H.;Mayahara,H.
  14. Biol. Reprod. v.43 Quantitative and qualitative characteristics of the stages and transitions in the cycle of the rat seminiferous epithelium: Light microscopic observation of perfusion-fixed and plastic embedded testis Hess,R.A. https://doi.org/10.1095/biolreprod43.3.525
  15. Biol. Reprod. v.43 Frequency of the stages in the cycle of the seminiferous epithelium in the rat Hess,R.A.;Schaeffer,D.J.;Eroschenko,V.P.;Keen,J.E. https://doi.org/10.1095/biolreprod43.3.517
  16. Histological methods for evaluation of the testis, Male Reproductive Toxicology Hess,R.A.;Moore,B.J.;Chapin,R.E.(ed.);Heindel,J.J.(ed.)
  17. Am. J. Pathol. v.42 The Sertoli cell index as a measure of testicular degeneration b zinc Kumi-Diaka,J.;Dennis,S.M.
  18. Toxicol. Pathol. v.30 Recommended approaches for the evaluation of testicular and epididymal toxicity Lanning,L.L.;Creasy,D.M.;Chapin,R.E.;Mann,P.C.;Barlow,N.J.;Regan,K.S.;Goodman,D.G. https://doi.org/10.1080/01926230290105695
  19. Repord. Toxicol. v.6 Endpoints of spermatotoxicity in the rat after short duration exposures to 14 reproductive toxicants Linder,R.E.;Starder,L.F.;Slott,V.L.;Suarez,J.D. https://doi.org/10.1016/0890-6238(92)90034-Q
  20. Am. J. Anat. v.90 Spermiogenesis of rat, mouse, hamster, and guinea pig as revealed by the periodic acid-fuchsin sulfurous acid technique Leblond,C.P.;Clermont,Y. https://doi.org/10.1002/aja.1000900202
  21. (EPA) 870.3800. Reproduction and Fertility Effects no.issued August OPPTS (Office of Prevention, Pesticides and Toxic Substances)
  22. Teratogenesis Carcinogen. Mutagen v.4 Reproductive risk assessment Overstreet,J.W. https://doi.org/10.1002/tcm.1770040108
  23. Risk Analysis v.8 Early indicators of male reproductive toxicity Overstreet,J.W.;Samules,S.J.;Day,P. https://doi.org/10.1111/j.1539-6924.1988.tb01150.x
  24. The ICH Guidelines for Detection of Toxicity to Reproduction for Medical Products (S5A) Including Addendum S5B and S5M(M): Toxicity on Male Fertility no.issued October;November
  25. The OECD 416 Two-generation Reproduction Toxicity Study no.issued January
  26. The OECD 421 and 422, The Repro/Developmental Toxicity Screening Test, and the Combined Repeated Dose Toxicity Study With Reproduction/Developmental Toxicity Screening Test no.issued July;March
  27. J. Toxicol. Sci.. Spec No:155-62 Collaborative work to evaluate toxicity on male reproductive organs by repeated dose studies in rats 15. Two-week and 4-week administration study of methyl methanesulfonate (MMS) Ozawa,S.;Yokoi,R.;Kitamura,T.;Kuriyama,K.;Kobayashi,K.;Shibata,N.
  28. J. Toxicol. Sci.. Spec No:241-9 Collaborative work to evaluate toxicity on male reproductive organs by repeated dose studies in rats 25. Effects of 2- and 4- week repeateddosing of dibromoacetic acid Tsuchiya,T.;Ooyama,N.;Murakami,T.;Sano,F.;Sugimoto,J.;Mutai,M.
  29. Scandinavian Journal of Work, Environment and Health v.23 Reproductive toxicity of 2-bromopropane Yu,I.J.;Chung,Y.H.;Lim,C.H.;Maeng,S.H.;Lee,J.Y.;Kim,H.Y.;Lee,S.J.;Kim,C.H.;Kim,T.G.;Lim,C.H.;Park,J.S.;Moon,Y.H. https://doi.org/10.5271/sjweh.221
  30. Tox. Letters v.109 Co-administration of toluene and xylene antagonized the testicular toxicity but not the hematopoietic toxicity caused by ethylene glycol monoethyl ether in Sprague-Dawley rats Yu,I.J.;Lee,H.Y.;Chung,Y.H.;Kim,K.J.;Han,J.H.;Cha,G.Y.;Chung,W.G.;Chan,Y.N.;Park,J.D.;Lee,Y.M.;Moon,Y.H. https://doi.org/10.1016/S0378-4274(99)00063-6
  31. PhD thesis, Cornell University Correlation of testicular pathology and reproductive performance in bulls used for artificial insemination Yuan,Y.D.
  32. Monographs on pathology of laboratory animals sponsored by the ILSI, Genital system Testicular degeneration, rat Yuan,Y.D.;McEntee,K.;Jones,T.C.(ed.);Mohr,U.(ed.);Hunt,R.D.(ed.)
  33. 精細管, Atlas 高橋道人