Biotechnology and Bioprocess Engineering:BBE

  • 제8권3호
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  • Pages.183-186
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  • 2003
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Production of Recombinant Hirudin in Galactokinase-deficient Saccharomyces cerevisiae by Fed-batch Fermentation with Continuous Glucose Feeding

  • Srinivas Ramisetti (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Bioechnology) ;
  • Kang, Hyun-Ah (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Bioechnology) ;
  • Rhee, Sang-Ki (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Bioechnology) ;
  • Kim, Chul-Ho (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Bioechnology)
  • 발행 : 2003.06.01
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초록

The artificial gene coding for anticoagulant hirudin was placed under the control of the GAL 10 promoter and expressed in the galactokinase-deficient strain (Δgal1) of Saccharomyces cerevisiae, which uses galactose only as a gratuitous inducer in order to avoid its consumption. For efficient production of recombinant hirudin, a carbon source other than galactose should be provided in the medium to support growth of the Δgal1 strain. Here we demonstrate the successful use of glucose in the fed-batch fermentation of the Δgal1 strain to achieve efficient production of recombinant hirudin, with a yield of up to 400 mg hirudin/L.

키워드

참고문헌

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