온도조건에 따른 담배거세미나방핵다각체병바이러스의 병원성 검정

Pathogenicity of Spodoptera litura Nucleopolyhedrovirus with Different Temperatures

  • 김선곤 (전남농업기술원 식물환경연구과) ;
  • 김도익 (전남농업기술원 식물환경연구과) ;
  • 박종대 (전남농업기술원 식물환경연구과) ;
  • 최형국 (전남농업기술원 식물환경연구과) ;
  • 유용만 (㈜경농중앙연구소)
  • 발행 : 2003.06.01

초록

담배거세미나방 핵다각체병바이러스의 온도별, 농도별로 병원성 검정결과는 다음과 같다. SINPV의 온도에 따른 담배거세미나방 유충 영기별 LC$_{50}$ 은 20-28$^{\circ}C$범위에서는 1,3,5령에서 온도가 높아질수록 낮아졌으나 32$^{\circ}C$에서는 높아졌다. LT$_{50}$ 은 핵다각체병바이러스 1.0$\times$$10^{5.7}$ Polyhedral inclusion bodies (PIBs)/ml에서 온도조건(20-32$^{\circ}C$)이 2$0^{\circ}C$와 24$^{\circ}C$에 비하여 28$^{\circ}C$와 32$^{\circ}C$에서 짧았다. 또한 바이러스 농도가 높고, 유충의 영기가 어릴수록 짧아져 LT$_{50}$ 은 온도조건, 바이러스 농도, 유충 영기에 의존하였다.

This experiment was conducted to investigate pathogenicity of Spodoptera litura nucleopolyhedrovirus (SINPV) with different temperatures to mass production. In laboratory, LC$_{50}$ values of SINPV were 5.534$\times$10$^3$ PIBs/ml and 4.l0$\times$10$^2$PIBs/ml in 1st instar larvae at 2$0^{\circ}C$ and 28$^{\circ}C$, respectively, but those were increased at 32$^{\circ}C$. LC$_{50}$ at 3rd and 5th instar larvae were showed the lowest value at 28$^{\circ}C$. LT$_{50}$ values of SINPV in 1.0$\times$10$^{5.7}$ PIBs/ml were determined at various temperature conditions (20-32$^{\circ}C$). The result showed that treatments at 28$^{\circ}C$ and 32$^{\circ}C$ shortened LT$_{50}$ values, but treatments at 2$0^{\circ}C$ and 24$^{\circ}C$ was relatively longer. Also LT$_{50}$ values was shortened in high concentration and young larva. LT$_{50}$ values are dependent on the temperature, viral concentration and larval instar.

키워드

참고문헌

  1. Bae, S.D. and H.J. Cho. 1998. Study on the physio-biology and control of Spodoptera litura Fabricius. Pl. Environ. Res. Rept. Nat. Yeongnam Agric. Expt. Sta. RDA. pp. 877-907
  2. Choi, J.Y., H.S. Kim, B.R. Jin, K.Y. Seol, H.Y. Park and S.K. Kang. 1996. Pathogenicity and production of Spodoptera exigua nuclear polyhedrosis virus. Korean. J. Appl. Entomol. 35: 228-231
  3. El-Saadany, G.B., G.M. Moaward, M.A. Rizk and S. Tantawy. 1992. The complex interaction between temperature and bioactivity of nuclear polyhedrosis virus infecting Spodoptera littoralis (Boisd.) larva. Egy. J. of Agri. Res. 70: 117-127
  4. Finney, D.J. 1971. Probit analysis (3rd Ed.). Cambridge Univ. Press, Cambridge 333pp
  5. Gelernter, W.D. and B.A. Federici. 1986. Isolation, identification and determination of virulence of a nuclear polyhedrosis virus from the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae). Environ. Entomol. 15: 240-245 https://doi.org/10.1093/ee/15.2.240
  6. Im, D.J., K.M. Choi, M.H. Lee, B.R. Jin and S.K. Kang. 1989. In vitro mass production of Spodoptera litura nuclear polyhedrosis virus. Korean J. Appl. Etomol. 28: 82-87
  7. Im, D.J., B.S. Park, B.R. Jin, K.M. Choi and S.K. Kang. 1988. Pathogencity of nuclear polyhedrosis virus isolated from the tobacco cutworm, Spodoptera litura. Korean. J. Appl. Entomol. 27: 219-224
  8. Kim, C.H. and H.Y. Shin. 1987. Studies on bionomics and control of tabacco cutworm, Spodoptera litura Fabricius in southern part of Korea. J. Insect. Sci. 5: 33-36
  9. Kurstak, E. 1982. In microbial and viral pesticides, pp. 335-507. Dekker. New York
  10. Oliver, A.D. 1964. Studies on the biological control of the fall wedworm, Hyphantria cunea, in Louisiana. J. Econ. Entomol 57: 314-318 https://doi.org/10.1093/jee/57.3.314
  11. SAS Institute. 1991. SAS/STAT user's guide: Statistics, version 6.04. SAS Institute, Cary, N.C
  12. Shin, H.Y., C.H. Kim, C.G. Park and Y.S. Lee. 1987. Biology of Tobacco Cutworm, Spodoptera litura (F.) (Lepidoptera: Noctuidae), 1. Seasonal Occurence of Tobocco Cutworm in Southern Korea and Larval Development, Pupal Period, Adult Longevity and Oviposition on the Different Food Sources. Res. Rept. RDA (D. M & U). 29: 301-307