Effect of $CO_2$-treatment on Oviposition and Colony Development of the Bumblebee, Bombus ignitus

탄산가스 처리가 호박벌(Bombus ignitus)의 산란성 및 봉세발달에 미치는 효과

  • 윤형주 (농촌진흥청 농업과학기술원 잠사곤충부 곤충자원과) ;
  • 김삼은 (농촌진흥청 농업과학기술원 잠사곤충부 곤충자원과) ;
  • 이상범 (농촌진흥청 농업과학기술원 잠사곤충부 곤충자원과) ;
  • 박인균 (농촌진흥청 농업과학기술원 잠사곤충부 곤충자원과)
  • Published : 2003.06.01

Abstract

The effect of $CO_2$-treatment on interrupting diapause of Bombus ignitus was examined to provide a means for year-round rearing of the bumblebee. When mated young queens were exposed to 65% or 99% $CO_2$ for 30 min daily during two consecutive days, oviposition rate increased to 75% and 77%, respectively, comparing 50% in $CO_2$-untreated queens. At the same time, the days needed to first oviposition shortened to 17-18 days in $CO_2$-treated queens, comparing to 30 days in $CO_2$-untreated queens. $CO_2$-treatment at the second day after mating was appropriate to the oviposition and colony development. $CO_2$-treatment showed a positive effect on the oviposition and colony development, but less than them of overwintered queen in numbers of produced progeny. It can be concluded that $CO_2$-treatment to B. ignitus is insufficient to produce commercial grade bumblebee colony in spite of its capability for promoting oviposition, because the treatment failed to form a big colony.

호박벌(Bombus ignitus)에 대한 탄산가스의 휴면타파 효과를 검토하였다. 65%, 99% 농도의 탄산가스처리 결과, 산란율은 무처리구의 50%에 비하여 65%와 99%의 탄산가스에 접촉시킨 시험구에서 각각 75%와 77%로 증가하였고, 첫산란소요일수 또한 무처리구에서 30일이 걸린데 비하여 65%와 99%의 시험구에서는 각각 18일과 17일로 단축되어 뚜렷한 탄산가스 처리효과가 나타났다. 그러나 65%와 99%의 시험구 간에는 차이가 보이지 않아 65-99%범위에서는 어느 농도의 탄산가스를 사용해도 좋을 것으로 생각되었다. 또한 탄산가스 최적 처리시기를 구명하기 위하여, 교미 후 1일부터 4일째까지 탄산가스를 처리한 결과, 산란성과 봉세발달이 교미 후 2일째가 가장 우수하여 최적 시기로 판명되었다. 그러나 호박벌에 대한 탄산가스 처리는 산란성과 봉세발달에는 긍정적인 효과를 보였지만, 차세대 출현수가 월동 여왕벌보다 적어 탄산가스 처리만으로는 상품성 있는 호박벌 봉군을 연중 생산하는 방법으로 부적당한 것으로 판단되었다.

Keywords

References

  1. Andrewartha, H.G. 1952. Diapause in relations to the ecology of insects. Biol., Rev. 27: 50-107 https://doi.org/10.1111/j.1469-185X.1952.tb01363.x
  2. Heinrich, B. 1979. Bumblebee economics, pp. 207-213. Harvard University Press. Cambridge, Massa
  3. Mansigh, A. 1971. Physiological classification of dormancy in insects. Can. Entomol. 103: 983-1009 https://doi.org/10.4039/Ent103983-7
  4. Minitab Inc. 2000. Minitab user's guide, Minitab Inc. USA
  5. Ono, M. 1997. Ecological implications of introducing Bombus terrestris, and significance of domestication of Japanese native bumblebee (Bombus spp.). pp. 244-252. In Proc Int. Workshop on Biological Invations of Ecosystem by Pests and Beneficial Organism. eds. by NIAES, Ministry of Agriculture, Forestry and fisheries. Tsukuba, Japan
  6. Pomeroy, N. and R.C. Plowright. 1979. Larval injection following $CO_2$ narcosis of bumblebees (Hymenoptera: Apidae). J. Kansas Entomol. Soc. 52: 215-217
  7. R$\ddot{o}$seler, P.F. 1985. A technique for year-round rearing of Bombus terrestris (Apidae, Bombini) colonies in captivity. Apidolo. 16: 165-170 https://doi.org/10.1051/apido:19850206
  8. R$\ddot{o}$seler, P.F. and I. R$\ddot{o}$seler. 1984. Effects of carbon dioxide and brain cauterization on corpora allata activity and oogenesia in bumblebees (Bombus hypnorum and Bombus terrestris). Zool. Jb. Physiol. 88: 237-246
  9. Tasei, J.N. 1994. Effect of different narcosis procedures on initiating oviposition of prediapausing Bomhus terrestris L. queens. Entomol. Exp. Appl. 72: 273-279 https://doi.org/10.1007/BF02383815
  10. van den Eijnde, J.A., de Ruijter and J. van der Steen. 1991. Method for rearing Bombus terrestris continuously and the production of bumblebee colonies for pollination purposes. Acta Horticulturae 288: 154-158
  11. Yoon, H.J., Y.I. Mah and M.Y. Lee. I.G. Park and M. Bilinski. 1999. Ecological characteristics of Bombus ignitus Smith in Korea. Korean J. Appl. Entomol. 38: 101-107
  12. Yoon, H.J. and S.E. Kim 2002. Facilitating effects of helpers on oviposition and colony development of bumblebee queen, Bombus ignitus. Koeran J. Appl. Entomol. 41: 239-245