A Study on Vitamin A Content of Mare Milk using HPLC

HPLC를 이용한 마유의 Vitamin A 함량 분석

  • Published : 2003.06.01

Abstract

Mare milk has a unique composition compared to other animal milks. This study was to determine the content of vitamin A in mare milk using HPLC and compared with cow milk The RT(retention time) of vitamin A by HPLC was about 4.4 min in mare and cow milk The results showed that vitamin A content of cow milk was higher than that of mare milk in each gram of milk sample. And the vitamin A content of mare cream was lower than that of cow cream in each gram of lipid. Consequently, vitamin A content of cow milk was higher than that of mare milk.

마유는 다른 동물의 젖과는 달리 매우 독특한 구성성분을 이루고 있다. 이 연구의 목적은 HPLC를 이용하여 마유의 비타민 A 함량을 결정하고, 우유의 비타민 A 함량과 비교분석하기 위한 것이다. HPLC를 이용하여 마유와 우유의 비타민A 함량을 분석한 결과 RT는 약 4.4 분이었다. 그리고 시료 100g당 우유와 마유의 비타민 A 함량은 각각 112 I.U, 42 I.U로서 우유가 마유보다 약 2.7배 가량 높았으며, 지방 1g당 비타민 A 함량은 우유가 30.41 I.U, 마유가 27.99 I.U로 마유가 우유보다 약간 적은 것을 알 수 있었다. 결과적으로 우유의 비타민 A 함량은 마유의 비타민 A 함량보다 높았다.

Keywords

References

  1. Csapo, J., Stefler, Martin, T.G., Makray, S., and Csapo-Kiss, Zs. (1995) Composition of mare's colostrum and milk. fat content, fatty acid composition and vitamin content, Int. Dairy Journal 5, 393-402 https://doi.org/10.1016/0958-6946(94)00008-D
  2. Csapo-Kiss, Zs., Stefler, J., Martin, T. G., Makray, S., and Csapo, J. (1995) Composition of mare's colostrum and milk. protein content, amino acid composition and contents of macro and micro elements, Int. Dairy Journal 5, 403-415 https://doi.org/10.1016/0958-6946(94)00014-G
  3. Curadi, M. C., Orlandi, M., Greppi, G. F., and Toppino, P. M. (2000) Identification of protein fractions in mare's colostrum and milk. Milchwissenschaft 55(8), 446-449
  4. Doreau, M., Boulot, S., Barlet, J. P., and Patureau-Mirand, P. (1990) Yield and composition of milk from lactating mares: effect of lactation stage and individual differences. J. Dairy Res. 57, 449-53 https://doi.org/10.1017/S0022029900029496
  5. Johnston, R. H., Kamstra, L. D., and Kohler, P. H. (1970) Mares milk composition as related to foal heat scours. J. Anim. Sci. 31, 549-553 https://doi.org/10.2527/jas1970.313549x
  6. Marconi, E. and Panfili, G.(1998) Chemical composition and nutritional properties of commercial products of Mare Milk Powder. Journal of Food Composition and Anatysis 11, 178-187 https://doi.org/10.1006/jfca.1998.0573
  7. Sliva, M. G., Green, A. E. and Sanders, J. K. (1992) Reversed-phase liquid chromatographic determination of vitamin D in infant formulas and enteral nutritionals. J. of AOAC International 75(3), 566-571
  8. Rovira, R., Ribera, F. Sanchis. V., and Canela, R. (1993) Improvements in the quantification of patulin in apple juice by high-performance liquid chromatography. J. Agric. Food Chem. 41, 214-219 https://doi.org/10.1021/jf00026a013
  9. Scott, J. K., Bishop, D. R., Zechalko A., and EdwardsWebb. J. D. (1984) Nutrition content of liquid milk vitamin A, D, C and of the B-complex in pasteurized bulk liquid milk. J. Dairy Res. 51, 37-50 https://doi.org/10.1017/S002202990002330X
  10. Solaroli, G., Pagliarmi, E., and Peri, C. (1993) Composition and nutritional quality of mare milk. Italian Journal of Food Science 1, 3-10 https://doi.org/10.1111/j.1365-2621.1936.tb17765.x
  11. Tanner, J. T. and Barnett, S. A. (1993) Analysis of milk-based infant formula. Phase V. vitamin A and E, folic Acid, and phantothenic acid: Food and Drug Administration-Infant Formula Council: Collaborative study. J. of AOAC International 76(2), 300-413
  12. Ullrey, D. E., Sruther, R. D., Hendricks, D. G., and Brent, B. E.(1966) Composition of mares milk. J. Anim. Sci. 25, 217-222 https://doi.org/10.2527/jas1966.251217x
  13. Virginia, A. T. (1990) Liquid chromatography and fluorescence detection of vitamin A in animal feeds, finished feeds, and premixes, J. Assoc. Off. Anal. Chem. 73(3), 463-466
  14. Wollard, D.C. and Indyk, H. (1986) The HPLC analysis of vitamin A isomers in dairy products and their significance in biopotency estimations. J. Micr. Anal. 2, 125-146
  15. Zahar, M. and Smith, D. E. (1990) Vitamin A quantification in fluid dairy products: Rapid method for vitamin A extraction for high performance liquid chromatography. J. of Dairy Science 73(12), 3402-3407 https://doi.org/10.3168/jds.S0022-0302(90)79036-4