미생물학회지 (Korean Journal of Microbiology)

  • 제39권2호
  • /
  • Pages.108-113
  • /
  • 2003
  • /
  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

디젤유 분해균주의 특성 및 토양배양

Characterization of Diesel Oil-Degrading Bacteria

  • 안민정 (명지대학교 생명과학과) ;
  • 한윤전 (명지대학교 생명과학과) ;
  • 임현섭 (주식회사 효성 화학연구소 BIO연구팀) ;
  • 최기현 (주식회사 효성 화학연구소 BIO연구팀) ;
  • 권오범 (주식회사 효성 화학연구소 BIO연구팀) ;
  • 정병철 (명지대학교 생명과학과)
  • 발행 : 2003.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

디젤유로 오염된 토양으로부터 분리한 디젤 분해 우수 균주를 HS 균주로 명명하고, 각 균주의 디젤유 분해능과 특성을 조사하였다. 분리된 HS균주의 동정결과 HSI 균주는 Acinetobacter sp. HS2, HS3 균주는Pseudomonas sp.로 동정되었다. 최소배지에서 디젤유2%, pH 7.0,$25^{\circ}C$, 교반속도 200 rpm의 조건으로 5일간 배양한 결과 HSI 균주는 88% 이상의 높은 분해효율을 나타내었다. 소수성과 유화능의 측정 결과 HSI 균주가 가장 높은 소수성을 나타내었고, 유화능은 HS3 균주가 가장 높게 나타났다. 위의 결과를 토대로 액체 배양시 분해효율이 가장 높은 HSI 균주를 선택하여 토양배양을 실시한 결과 30일이 경과된 후 80%이상의 디젤유 분해효율을 나타내었고, 디젤유 분해효율은 미생물 활성과 비례하는 것으로 확인되었다. 따라서 신규 분리된 디젤유 분해균주는 높은 디젤유 분해능과 토양 생존능으로 실제 유류오염 환경에 적용이 가능할 것으로 사료된다.

Diesel oil-degrading bacterial strains were isolated from diesel oil contaminated soil and called HS series (HS1, HS2 and HS3). These strains were identified as Acinetobacter sp. (HS1) and Pseudomonas sp. (HS2 and HS3) based on Biolog test, cellular fatty acid composition, and 16S rDNA sequence analysis. These strains were coltivated in liquid minimal media containing 2% diesel oil, and diesel oil-degrading activity was measured. As result, all strains degraded over 70% of total diesel oil. But PAH (polycyclic aromatic hydrocarbon)- and pris- tane-degrading rate of these strain was below 20％ of total PAH and pristane. The HS 1 strain showed highest hydrophobicity and low emulsifying activity among the experimental strains and high diesel oil-degrading activity. From the above-mentioned result, microcosm experiment was performed with the HS1 strain. The HS1 strain showed a degrading activity of over 80% of total diesel oil in microcosm test. And microbial activity was correlated to diesel oil-degrading activity. Therefore, it is suggested that the HS1 strains could be effectively used for the bioremediation for diesel oil.

키워드

참고문헌

  1. 한국생물공학회지 v.13 Hydrocarbon uptake modes에 따른 유류분해 미생물 혼합제의 원유분해능 고성환;이홍금;김상진
  2. 한국미생물학회지 v.32 토양미생물군집의 개체수와 활성도에 미치는 경유의 영향 서은영;송홍규
  3. 한국미생물학회지 v.33 Tsukamurella sp.26A에 의한 계면활성제의 생산 최경숙;김순한;정영기;장경립;이태호
  4. Appl. Microbiol. Biotechnol. v.45 Optimizing biodegradation of phenanthrene dissolved in non aqueous - phase liquids Birman,I;M.Alexander https://doi.org/10.1007/s002530050682
  5. Appl. Environ. Microbiol. v.58 Enumeration of phenanthrene - degrading bacteria by an overlayer technique and its use in evaluation of petroleum - contaminated sites Bogardt,A.H.;B.B.Hemmingsen
  6. Lett. Appl. Microbiol. v.32 Comparison of a range of green fluorescent protein-tagging vectors for monitoring a microbial inoculant in soil Dandie,C.E.;S.M.Thomas;N.C.McClure https://doi.org/10.1046/j.1472-765x.2001.00848.x
  7. J. Inst. Microbiol. v.16 Emulsifying activity in themophilic and extremely thermophlic microorganisms De Acevedo,G.T.;M.J.Mclnerney https://doi.org/10.1007/BF01569914
  8. Microbiol. Res. v.155 Detection and characterization of quorum sensing signal molecules in Acinetovacter stains Gonzalez,R.H.;A.Nusblat;B.C.Nudel https://doi.org/10.1016/S0944-5013(01)80004-5
  9. J. Chromatography A v.892 Comparisons of soxhlet extraction, pressurized liquid extraction, supercritical fluid extraction and subcritical water extraction for environmental solids: recovery, selectivity and effects on sample matrix Hawthorne,S.B.;C.B.Grabanski;E.Martin;D.J.Miller https://doi.org/10.1016/S0021-9673(00)00091-1
  10. Appl. Environ. Microbiol. v.61 Effect of environmental parameters on the formation and turnover of acetate by forest soils Kirsten,K.;H.L.Drake
  11. Chemosphere v.30 Biodegradation studies of hydrocarbons in soils by analyzing metabolites formed Langbehn,A.;H.Steinhart https://doi.org/10.1016/0045-6535(94)00446-2
  12. Appl. Microbiol. Biotechnol. v.51 Bioavailability of hydrocarbons during microbial remediation of a sandy soil Loser,C.;H.Seidel;P.Hoffmann;A.Zehnsdorf https://doi.org/10.1007/s002530051370
  13. Chemosphere v.38 Biodegradation of diesel oil by cold-adapted microorganisms in presence of sodium dodecyl sulfate Margesin,R.;F.Schinner https://doi.org/10.1016/S0045-6535(98)00575-X
  14. Appl. Microbiol. Biotechnol. v.47 Bioremediation of diesel-oil-contaminated alpine soils at low tempterature Margesin,R.;F.Schinner https://doi.org/10.1007/s002530050957
  15. Curr. Opin. Biotech. v.11 Biodesulfurization and the upgrading of petroleum distillates Monticello,D.J. https://doi.org/10.1016/S0958-1669(00)00154-3
  16. Chemosphere v.41 Bioremediation of a soil contaminated by hydrocarbon mixtures: the residual concentration problem Nocentini,M.;D.Pinelli;F.Fava https://doi.org/10.1016/S0045-6535(00)00057-6
  17. Proc. Natl. Acad. Sci. v.91 Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes Pearson,J.P.;K.M.Gray;L.Passador;K.D.Tucker;A.Eberhard;B.H.Iglewsand;E.P.Greenberg https://doi.org/10.1073/pnas.91.1.197
  18. Proc. Natl. Acad. Sci. v.92 A second N-Acyl homoserine lactone signal produced by Pseudomonas aeruginosa Pearson,J.P.;L.Passador;B.H.Iglewski;E.P.Greenberg https://doi.org/10.1073/pnas.92.5.1490
  19. Biodegradation v.7 Enhanced biodegradation of diesel fuel through the addition of particulate organic carbon and inorganic nutrients in coastal marine waters Piehler,M.F.;H.W.Paerl https://doi.org/10.1007/BF00058183
  20. Microb. Ecol. v.38 Stimulation of diesel fuel biodegradation by indigenous nitrogen fixing bacterial consortia Piehler,M.F.;J.G.Swistak;J.L.Pinckney;H.W.Paerl https://doi.org/10.1007/s002489900157
  21. Microbiol. Res. v.150 Degradation of refinery products and oils from polluted sites by the autochthonous microorganisms of contaminated and pristine soils Riis,V.;D.Miethe;W.Babel https://doi.org/10.1016/S0944-5013(11)80013-3
  22. Curr. Opin. Biotech. v.8 Bioemulsans microbial polymeric emulsifiers Rosenberg,E.;E.Z.Ron https://doi.org/10.1016/S0958-1669(97)80009-2
  23. FEMS Microbial Lett. v.9 Adherence of bacteria to hydrocarbons : a simple method for mesuring cell surface hydrophobicity Rosenberg,M.;M.Gutnick;E.Rosenberg https://doi.org/10.1111/j.1574-6968.1980.tb05599.x
  24. Analyst v.121 Biodegradation studies of selected hydrocarbons from diesel oil Sepic,E.;C.Trier;H.Leskovsek https://doi.org/10.1039/an9962101451
  25. Curr. Opin. Biotech. v.8 Polycyclic hydrocarbon bioremediation design Harayama,S. https://doi.org/10.1016/S0958-1669(97)80002-X
  26. Chemosphere v.39 A bench-scale investigation of land treatment of soil contaminated with diesel fuel Taylor,C.;T.Viraraghavan https://doi.org/10.1016/S0045-6535(99)00056-9
  27. Appl. Biochem. Biotechnol. v.70 Effect of drying on bioremediation bacteria properties Weekers,F.;P.H.Jacques;D.Springael;M.Mergeay;L.Diels;P.H.Thonart https://doi.org/10.1007/BF02920147
  28. Poult. Sci. v.78 The use of poultry litter as co - substrate and source of inorganic nutrients and microorganisms for the ex situ biodegradation of petroleum compounds Williams,C.M.;J.L.Grimes;R.L.Mikkelsen https://doi.org/10.1093/ps/78.7.956
  29. Appl. Environ. Microbiol. v.60 Effect of a Pseudomonas rhamnolipid biosurfactant on cell hydrophobicity and biodegradation of octadecane Zhang,Y.;R.M.Miller