Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effectiveness of Bioremediation on Oil-Contaminated Sand in Intertidal Zone

  • Oh, Young-Sook (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Sim, Doo-Suep (Microbiology Laboratory, Korea Ocean Research and Development and Development Institute) ;
  • Kim, Sang-Jin (Microbiology Laboratory, Korea Ocean Research and Development and Development Institute)
  • Published : 2003.06.01
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Abstract

Bioremediation technologies were applied to experimental microcosms, simulating an oil spill in a lower intertidal area. Three treatments (oil only, oil plus nutrients, and oil plus nutrients and microbial inocula) were applied, and each microcosm was repeatedly filled and eluted with seawater every 12 h to simulate tidal cycles. To minimize washing-out of the inoculum by the tidal cycles, microbial cells were primarily immobilized on diatomaceous earth before they were applied to the oiled sand. Oil degradation was monitored by gravimetric measurements, thin layer chromatography/flame ionization detector (TLC/FID) analysis, and gas chromatography (GC) analysis, and the loss of oil content was normalized to sand mass or nor-hopane. When the data were normalized to sand mass, no consistent differences were detected between nutrient-amended and nutrient/inoculum-amended microcosms, although both differed from the oil-only microcosm in respect of oil removal rate by a factor of 4 to 14. However, the data relative to nor-hopane showed a significant treatment difference between the nutrient-amended and nutrient/inoculum-treated microcosms, especially in the early phase of the treatment. The accelerating effect of inoculum treatment has hardly been reported in studies of oil bioremediation in the Tower intertidal area. The inoculum immobilized on diatomaceous earth seemed to be a very effective formulation for retaining microbial cells in association with the sand. Results of this study also suggest that interpretation of the effectiveness of bioremediation could be dependent on the selection of monitoring methods, and consequently the application of various analytical methods in combination could be a solution to overcome the limitations of oil bioremediation monitoring.

Keywords

References

  1. Nature v.368 Effectiveness of bioremediation for the Exxon Valdez oil spill Bragg,J.R.;R.C.Prince;E.J.Harner;R.M.Atlas https://doi.org/10.1038/368413a0
  2. Appl. Environ. Microbiol. v.56 Sheen screen, a miniaturized most-probable-number method for enumeration of oil-degrading microorganisms Brown,E.J.;J.F.Braddock
  3. J. Microbiol. Biotechnol. v.12 Evaluation of fertilizer additions to stimulate oil biodegradation in sand seashore mesocosms Choi,S.C.;K.K.Kwon;J.H.Sohn;S.J.Kim
  4. Bull. Environ. Contam. Toxicol. v.49 Effectiveness of a bioremediation product in degrading the oil spilled in the 1991 Arabian Gulf war Fayad,N.M.;R.L.Edora;A.H.El-Mubarak;A.B.Polancos
  5. J. Mar. Biotechnol. v.2 TLC/FID method for evaluation of the crude-oil-degrading capability of marine microorganisms Goto,M.;M.kato;M.Asaumi;K.Shirai;K.Venkateswaran
  6. Microbiology v.54 Biodegradation of oil hydrocarbons in soil inoculated with yeasts Ismailov,N.M.
  7. Appl. Environ. Microbiol. v.27 Effect of amendments on the microbial utilization of oil applied to soil Jobson,S.;M.McLaughlin,F.D.Cook;D.W.S.Westlake
  8. J. Microbiol. Biotechnol. v.11 Evaluation of bioremediation effectiveness by resolving rate-limiting parameters in diesel-contaminated soil Joo,C.S.;Y.s.Oh;W.J.Chung
  9. New Developments in Marine Biotechnology The effects of bioremediation on the oil degradation in oil polluted environments Kim,S.J.;J.H.Sohn;D.S.Sim;K.K.Kwon;T.H.Kim;Y. Le Gal(ed.);H.O.Halvorson(ed.)
  10. J. Microbiol v.37 Identification and characterization of an oil-degrading yeast. Yarrowia lipolytica 180 Kim,T.H.;J.H.Lee;Y.S.Oh;K.S.Bae;S.J.Kim
  11. J. Microbiol. Biotechnol. v.10 The possible involvement of the cell surface in aliphatic hydrocarbon utilization by an oil-degrading yeast, Yarrowia lipolytica 180 Kim,T.H.;Y.S.Oh;S.J.Kim
  12. Practical Environmental Bioremediation King,R.B.;G.M.Long;J.K.Sheldon
  13. Biotech. Lett. v.23 Specific detection of an oil-degrading bacterium, Corynebacterium sp. IC10, in sand microcosms by PCR using species-specific primers based on 16S rRNA gene sequences Lee,J.H.;S.Y.Jeong;S.J.Kim
  14. J. Microbiol. Biotechnol. v.11 Bioremediation of diesel-contaminated soil by bacterial cells transported by electrokinetics Lee,H.S.;K.Lee
  15. Pure Appl. Chem. v.71 Bioremediation of oil on shoreline environments: Development of techniques and guidelines Lee,K.;F.X.Merlin https://doi.org/10.1351/pac199971010161
  16. Third International In situ and on-situ Bioreclamation Symposium Proceedings v.3 Assessing bioremediation of crude oil in soils and sludges McMillen,S.J.;N.R.Gray;J.M.Kerr;A.G.Requejo;T.J.McDonald;G.S.Douglas
  17. J. Ind. Micrbiol. v.10 Effect of inoculation on the biodegradation of weathered prudhoe bay crude oil Mue ler,J.F.;S.M.Resnick;M.E.Shelton;P.H.Pritcnard https://doi.org/10.1007/BF01583841
  18. Biotech. Lett. v.22 Biological treatment of oil-contaminated sand: Comparison of oil degradation based on thin-layer chromatography/flame ionization detector (TLC/FID) and respirometric analysis Oh,Y.S.;W.Y.Choi;Y.H.Lee;S.C.Choi;S.J.Kim https://doi.org/10.1023/A:1005657030335
  19. Marine Pollut. Bull. v.42 Effects of nutrients on crude oil biodegradation in the upper intertidal zone Oh,Y.S.;D.S.Sim;S.J.Kim https://doi.org/10.1016/S0025-326X(01)00166-7
  20. Mar. Environ. Res. v.45 Weathering rates of oil components in a bioremediation experiment in estuarine sediments Oudot,J.;F.X.Merlin;P.Pinvidic https://doi.org/10.1016/S0141-1136(97)00024-X
  21. The Biomarker Guide;Interpreting molecular fossils in petroleum and ancient sediments Peters,K.;J.M.Moldowan
  22. Environ. Sci. Technol. v.28 17α(H),21β(H)-Hopane as a conserved internal marker for estimating the biodegradation of crude oil Prince,R.C.;D.L.Elmendorf;J.R.Lute;C.S.Hsu;C.E.Halth;J.D.Senlus;G.J.Dechert;G.S.Douglas;E.L.Butler https://doi.org/10.1021/es00050a019
  23. Crit. Rev. Microbiol. v.19 Petroleum spill bioremediation in marine environments Prince,R.C. https://doi.org/10.3109/10408419309113530
  24. Chemosphere v.26 Further research into the aerobic degradation of n-alkanes in a heavy oil by a pure culture of a Pseudomonas sp. Setti,L.;G.Lanzarini;P.G.Pifferi;G.Spagna https://doi.org/10.1016/0045-6535(93)90202-G
  25. J. Microbiol. v.37 Biodegradation of phenanthrene by Sphingomonas sp. strain KH3-2 Shin,S.K.;Y.S.Oh;S.J.Kim
  26. Microb. Ecol. v.8 Measurement of electron transport system (ETS) activity in soil Trevors,J.T.;C.I.Mayfield;W.E.Inniss https://doi.org/10.1007/BF02010449
  27. Environ. Sci. Technol. v.30 Bioremediation of an experimental oil spill on the shoreline of Delaware Bay Venosa,A.D.;M.T.Suidan;B.A.Wrenn;K.L.Strohmeier;J.R.Haines;B.L.Eberhart;D.King;E.Holder https://doi.org/10.1021/es950754r
  28. Hydrobiol. Bull. v.16 Respiratory electron transport system activities in marine environments Vosjan,J.H. https://doi.org/10.1007/BF02255413
  29. Estuaries v.11 NOAA Gulf of Mexico status and trends program: Trace organic contaminant distribution in sediments and oysters Wade,T.L.;E.L.Atlas;J.M.Brooks;M.C.Kennicutt Ⅱ;R.G.Fox;J.Sericano;B. Garcia-Romero;D.DeFreitas https://doi.org/10.2307/1351969