Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Detection of MecA Gene in Clinical Isolates of Staphylococcus aureus by Multiplex-PCR, and Antimicrobial Susceptibility of MRSA

  • Lee, Hyean-Woo (Department of Biochemistry, Institute of basic Medical Science, Yonsei University Wonju College of Medicine) ;
  • Yoon, Joon-Ho (Department of Biochemistry, Institute of basic Medical Science, Yonsei University Wonju College of Medicine) ;
  • Sohn, Joon-Hyung (Department of Biochemistry, Institute of basic Medical Science, Yonsei University Wonju College of Medicine) ;
  • Lee, Kyoung-Ho (Department of Microbiology, Institute of basic Medical Science, Yonsei University Wonju College of Medicine) ;
  • Yeh, Byung-Il (Department of Biochemistry, Institute of basic Medical Science, Yonsei University Wonju College of Medicine) ;
  • Park, Deok-Woo (Department of Biochemistry, Institute of basic Medical Science, Yonsei University Wonju College of Medicine) ;
  • Kim, Hyun-Won (Department of Biochemistry, Institute of basic Medical Science, Yonsei University Wonju College of Medicine) ;
  • Choi, Jong-Whan (Department of Biochemistry, Institute of basic Medical Science, Yonsei University Wonju College of Medicine)
  • Published : 2003.06.01
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Abstract

Multiplex-PCR protocols were designed in order to make a rapid identification of MRSA. MecA, femB, and 165 rRNA genes were amplified for making a detection of MRSA. The incidence of MRSA in the clinical isolates of Staphylococcus aureus was examined by using a multiplex-PCR assay. The mecA gene was detected in 266 strains out of 336 clinical isolates of S. aureus, thus the incidence of MRSA was approximately 76.5%. The MRSAs of 247 strains (96.1%) showed resistance to more than eight species of the antimicrobial agents tested. The isolates of MRSA showed 27 different antimicrobial-resistant patterns. The results indicate that many different MRSA strains having high multidrug resistance are actually prevalent in Korea. Also, VISA was screened from the MRSA. Two strains were grown on the BHI agar plate supplemented with $8\;\mu\textrm{g}/ml$ of vancomycin at a frequency of $1/10^8$ colony forming units or higher.

Keywords

References

  1. J. Clin. Microbiol. v.30 Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene Brakstad,O.G.;K.Aasbakk;J.A.Maeland
  2. Microbiol. Rev. v.51 Antimicrobial resistance of Staphylococcus aureus: Genetic basis Bruce,R.;L.R.Skurray
  3. J. Korea Med. Sci. v.3 In vitro activities of eight antibiotics against methicillin-resistant Staphylococcus aureus and S. epidermidis strains isolated in Korea Chang,W.H.;M.S.Choi;H.Y.Chung;W.J.Seo;T.Y.Choi;Y.S.Chong;J.S.Kim;S.H.Hong
  4. Science v.264 Inactivation of antibiotics and the dissemination of resistance genes Davies,J. https://doi.org/10.1126/science.8153624
  5. Clin. Microbiol. Rev. v.13 Exotoxins of Staphylococcus aureus Dinges,M.M.;P.M.Orwin;P.M.Schlievert https://doi.org/10.1128/CMR.13.1.16-34.2000
  6. Antimicrob. Agents Chemother v.43 Genetic methods for assessing antimicrobial resistance Franklin,R.;I.Cockerill
  7. J. Clin. Microbiol v.32 Multiplex PCR of identification of methicillin-resistant staphylococci in the clinical laboratory Geha,D.J.;J.R.Uhl;C.A.Gustaferro;D.H.Persing
  8. J. Bacteriol v.158 Low affinity penicillin-binding protein associated with β-lactam resistance in Staphylococcus aureus Hartman,B.J.;A.Tomasz
  9. J. Microbiol. Biotechnol v.8 Purification and characterization of antistaphylococcal substance from Pseudomonas sp. KUH-001 Hwang,S.Y.;S.H.Lee;K.J.Song;Y.P.Kim;K.Kawhara
  10. Yonsei Med. J. v.39 Acquisition of methicillin resistance and progression of multi-antibiotic resistance in methicillin-resistant Staphylococcus aureus Ito,T.;H.Keiichi
  11. J. Microbiol. Biotechnol v.11 Antibacterial effect of the surface-modified biomedical polyurethane against Staphylococcus aureus and Staphylococcus epidermidis Jeon,S.M.;H.J.Kim;K.B.Lee;J.W.Kim;M.N.Kim
  12. Eur. J. Clin. Microbiol. Infect. Dis. v.18 Evaluation of the mecA, femB duplex polymerase chain reaction for detection of methicillin-resistant Staphylococcus aureus Jonas,D.;D.H.Grundmann;F.D.Daschner;K.J.Towmer https://doi.org/10.1007/s100960050365
  13. Korea J. Clin. Pathol v.18 The trends of the species and antimicrobial susceptibility of bacteria and fungi isolated from blood cultures (1986-1996) Kang B.K.;H.J.Lee;J.T.Suh
  14. J. Hospital Infect v.43 Rapid detection of methicillin-resistant Staphylococci by multiplex PCR Kearns,A.M.;P.R.Seiders;J.Wheeler;R.Freeman;M.Steward https://doi.org/10.1053/jhin.1999.0631
  15. J. Microbiol. Biotechnol v.11 Comparison of different PCR-based genotyping techniques for MRSA discrimination among methicillin-resistant Staphylococcus aureus isolates Kim,K.S.;H.A.Seo;C.Y.Oh;H.Kim;J.B.Kim
  16. J. Clin. Microbiol v.38 Vancomycin-intermediate Staphylococcus aureus in Korea Kim,M.N.;C.H.Hiramtsu
  17. Epidemiol. Infect. v.113 Detection of mecA, femA and femB genes in clinical strains of staphylococci using polymerase chain reaction Kobayashi,N;H.Wu;K.Kojima;K.Taniguchi;S.Urasawa;N.Uehara;Y.Omizu;Y.Kishi;A.Yagihashi;I.Kurokawa https://doi.org/10.1017/S0950268800051682
  18. Chemoth. J. v.8 Resistenzentwicklung bei Staphylokokken und anderen grampositiven Erregern gegenubr chemotherapeutika im mitteleuropaischen raum Kresken,M.;D.Hafner;die Studiengroup
  19. Lancet v.357 Whole genome sequencing of methicillin-resistant Staphylococcus aureus Kuroda,M.(et al.) https://doi.org/10.1016/S0140-6736(00)04403-2
  20. Korea J. Clin. Pathol v.17 Restriction endonuclease analysis of plasmids and antimicrobial resistance pattern of Staphylococcus aureus and Staphylococcus epidermidis isolated from clinical specimens Lee,H.J.;J.R.Lee;M.H.Kim;J.T.Suh;Y.I.Kim;H.J.Suh
  21. J. Korea Med. Sci. v.16 Typing and antimicrobial susceptibilities of mithicillin-resistant Staphylococcus aureus strains isolated in a hospital in Korea Lee,H.J.;Y.S.Kim;W.Lenz;G.Bierbaum;K.P.Schaal
  22. J. Microbiol. Biotechnol v.7 A peptide antibiotic AMRSA1 active against multidrug-resistant Staphylococcus aureus produced by Streptomyces sp. HW-003 Lee,H.W.;J.W.Choi;H.W.Kim;D.P.Han;W.S.Shin;D.H.Yi
  23. J. Microbiol. Biotechnol v.12 Expression of sortase, a transpeptidase for cell wall sorting reaction, from Staphylococcus aureus ATCC6538p in Escherichia coli Lee,K.Y.;D.S.Shin;J.M.Yoon;H.Kang;K.B.Oh
  24. Clin. Microbiol. Infect v.7 The VISA/GISA problem: Therapeutic implications Linares,J.
  25. J. Clin. Microbiol v.38 Evaluation of three rapid methods for detection of methicillin resistance in Staphylococcus aureus Louie,L.;S.O.Matsumura;E.Choi;M.Louie;A.E.Simor
  26. J. Clin. Microbiol v.35 Heterogeneous vancomycin resistance in methicillin-resistant Staphylococcus aureus strains isolated in an Italian hospital Marchese,A;G.Balistreri;E.A.Debbia;G.C.Schito
  27. Int. J. Antimicrob. Agents v.18 Methicillin-resistant Staphylococcus aureus in the Hospitals of central Greece Petinaki,E.;V.Miriagou;L.S.Tzouvelekis;S.Pournaras;F.Hatzi;F.Kontos;M.Maniati;A.N.Maniatis;Bacterial resistance study group of Thessaly https://doi.org/10.1016/S0924-8579(01)00327-2
  28. The Molecular Basis of Pathogenicity Projan,S.J.;R.P.Novick
  29. FEBS Lett. v.192 Penicillin-binding proteins of β-lactam-resistant strains of Staphylococcus aureus Reynolds,P.E.;D.F.J.Brown https://doi.org/10.1016/0014-5793(85)80036-3
  30. Korean J. Infect. Dis. v.27 Antimicrobial resistance and plasmid profile of methicillin-resistant Staphylococcus aureus Ryu,P.Y.;Y.I.Kim;J.H.Rhee;S.S.Chung;T.H.Ahn;J.H.Shin;D.W.Ryang;Y.H.Kim https://doi.org/10.3109/00365549509018965
  31. J. Med. Microbiol. v.46 Specific information concerning taxonomy, pathogenicity and methicillin resistance of staphylococci obtained by a multiplex PCR Schmitz,F.J.;C.R.Mackenzie;B.Hofmann;J.Verhoef;M. Finken-Eigen;H.P.Heinz;K.Kohrer https://doi.org/10.1099/00222615-46-9-773
  32. Eur. J. Clin. Infect. Dis. v.17 Detection of Staphylococcal and peritoneal fluid samples using multiplex polymerase chain reaction Schmitz,F.J.;M.Steiert;B.Hofmann;A.C.Fluit;J.Verhoef;H.P.Heinz;M.E.Jones
  33. J. Clin. Microbiol v.39 Nosocomial spread of an unusual methicillin-resistant Staphylococcus aureus clone that is sensitive to all non-β-lactam antibiotics, including tobramycin Spyros,P.;A.Slavakis;A.Polyzou;D.Sofianou;A.N.Maniatis;A.Tsakris https://doi.org/10.1128/JCM.39.2.779-781.2001
  34. Diagn. Microbiol. Infect. Dis. v.42 Molecular epidemiology and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus in Taiwan Wang,J.T.;Y.C.Chen;T.L.Yang;S.C.Chang https://doi.org/10.1016/S0732-8893(01)00351-0