Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

The Effects of Korean Traditional Medicine Mixture on Cytokine Level, Food Intake and Nutrition Metabolism of the Cachexia Induced-Mice

혼합한약재가 악액질이 유도된 생쥐의 Cytokine분비 및 식이섭취와 영양대사에 미치는 영향

  • 왕수경 (대전대학교 이과대학 식품영양학과) ;
  • 윤은영 (대전대학교 이과대학 식품영양학과) ;
  • 박정민 (한국식품개발연구원) ;
  • 임종순 (대전대학교 동서생명과학연구원) ;
  • 김승형 (대전대학교 동서생명과학연구원)
  • Published : 2003.05.01
Download PDF
⟨ Previous Next ⟩

Abstract

Cancer cachexia, characterized by weight loss and progressive tissue wasting, has been postulated to be mediated by cytokines. This study was conducted to evaluate the effect of Korean Traditional Medicine (KTM ; mokhyang, jisil, osooyu) mixture on food intake, blood cytokines level and blood nutrients status of the cachexia induced-mice. Thirty male Balb/c mice aged 6-8 weets were blocked into 3 groups that were Normal (no colon26 cells) Control (colon 26 cells) and KTM (colon26 cells + KTM extract mixture) group. In Control and KTM groups, murine adenocarcinoma colon 26 cells were injected subcutaneously to induce cachexia. KTM mice were given 200 ul KTM extract mixture (7%) per day. Half of each groups were sacrificed at the 14 th day to see serum cytokines & nutrients and the others were fed until almost of control group died to see life span. food intake and body weight were decreased significantly in cachexia induced groups. Tumor weight of KTM group was significantly lower than control group. Serum cytokines (IL-1$\beta$ and TNF-$\alpha$) level of cachexia induced groups were increased than those of normal group, and those of KTM group were significantly lower than the level of control group. Total serum protein and serum albumin were higher in KTM group than other groups. TG and fatty acid were lower in cachexia induced groups than normal group. HDL-cholesterol in serum was increased in KTM group. Effect of oral administration of KTM extract mixture on survival time of colon26 bearing mice showed extension of the life span. Overall, this study showed that KTM (mokhyang, jisil, osooyu) extract mixture inhibited the growth of cancer cell, changed the secretion of cytokines induced by colon26 adenocarcinoma in mice, and changed nutrition metabolism.

Keywords

References

  1. Ministary of health and welfare. National nutrition survey report, 2000
  2. Grant M, Ropka ME. Alterations in nutrition. in McCorkle R, Grant M, Frank Stromborg M and Baird (Eds). S.B. Cancer Nursing, a comprehensive textbook (2nd ed.), Saunders, Philadelphia london, 1996
  3. Kim YO, Han B. Association of Nutritional Status with Clinical Outcome of Stomach Cancer Patients. J Korean Soc Food Sci Nutr 29(6): 1185-1189, 2000
  4. Burtis G, Caviw J, Martin S. Applied nutrition and diet therapy. W.B. Saunder Company. Philadelphia, pp.484-487, 1988
  5. Rivadeneria DE, Denis E, Thomas JF, Micheael DL, John MD. nutritional support of the cancer patients. CA Cancer J Clin 48: 69-80, 1998 https://doi.org/10.3322/canjclin.48.2.69
  6. Shils ME, Olson JA, Shike M. Modern nutrition in health and disease. Lea & Feriger, Philadelphia, pp. 1319-1326, 1994
  7. McWhirter JP, pennington CR. Incidence and recognition of malnutrition in hospita. BMJ 308: 945-948, 1994 https://doi.org/10.1136/bmj.308.6934.945
  8. Kim EK, Yang YH, Choi SM. Nutritional Status of Cancer Patients upon Admission. J Korean Nurses Association 29(2): 271-280, 1999
  9. Bruera E, Higginson I (eds). Cachexia-Anorexia in Cancer Patients. New York, Oxford University Press, pp.1-197, 1996
  10. Orosz P, Echtenacher B, Falk W, Ruschoff J, Weber D, Mannel DN. Enhancement of experimental metastasis by tumor necrosis factor. J Exp Med 177: 1391-1398, 1993 https://doi.org/10.1084/jem.177.5.1391
  11. Collini PL, De Giovanni C, Nioletti G, Bontadni A, Tazzari PL, Landuzzi L, Scotlandi K, Nanni P. Engancement of experimental metastatic activity by tumor necrosis factor-alpha alone or in combination with interferon-gamma. Cli Exp Metastasis 8: 215, 1992
  12. Iizumi T, Yazaki T, Umeda T, Soma GIC. Promotion of metastasis by tumor necrosis factor in rats bearing Dunning R3327 MAT-LyLu prostatic tumor. Urologia Internationalis 51:85, 1990 https://doi.org/10.1159/000125321
  13. Giavazzi R, Garofalo A, Bani MR, Abbate M, Grezzi P, Boraschi D, Mantovani A, Dejana E. Interleukin-1-induced augmentation of experimental metastasis from a human melanoma in nude mice. Cancer Res 50: 4771-4775, 1990
  14. Chirivi RG, Garofalo A, Padura IM, Mantovani A, Givazzi R. Interleukin-1 receptor antagonists inhibits the augmentation of metastasis induced by IL-1 or LPS in ahuman melanoma/nude mouse system. Cancer Res 55: 505, 1993
  15. McNamara JM, Alexander R, Norton JA. Cytokines and their role in the pathophysiology of cancer cachexia. Journal of parenteral and Enternal Nutrition 16: 505, 1992 https://doi.org/10.1177/0148607192016006505
  16. Moldawer LL, Rogy MA, Lowry SF. The role of cytokines in cancer cachexia. J Porenteral and Enteral Nutrition 16(6supp1): 43s-49s, 1992 https://doi.org/10.1177/014860719201600602
  17. Tisdale MJ. Cancer cachexia. Anti-cancer Drugs 2: 115-125, 1993
  18. Watanabe S, Bruera E. Anorexia and cachexia, asthenia, and lethargy. Hematology/Oncology Clinics of North America 10(1): 189-206, 1996 https://doi.org/10.1016/S0889-8588(05)70334-8
  19. Lee SW, Hwang BY, Kim SE, Kim HM, Kim YH. Isolation of Modulators for Multidrug Resistance from the Fruits of Evodia officinalis. J Kor Pharmacogn 26(4): 344-348, 1995
  20. Wasser SP, Weis AL. Therapeutic effects of substances occurring in higher basidiomycetes mushrooms. A modern perspective. Crit Rev Immunol 19: 65-96, 1999
  21. Kang SS, Kim JS, Chi HJ, Chang SY, Ha KW. Isolation and Quantitative Determination of Costunolide from Saussurea Root. J Kor Pharmacogn 30(1): 48-53, 1999
  22. Hong MD, Kim NJ, Kim DH, Yoon WG. Studies on the Antiinflammatory Constituents of Ponciri Fructus. J Kor Pharmacogn Abstracts of the 21st Annual Convention. p.64, 1991
  23. Park SY, Kim JW. Screening and Isolation of the Antitumor Agents from Medicinal Plants (1). J Kor Pharmacogn 23(4): 264-267, 1992
  24. Tanaka Y, Eda H, Tanaka T, Udagawa T, Ohiwa T, Tatsuno K, Tanaka Y, Ishitsuka H. Cytokines induce uridine phosphorylase in mouse colon 26 carcinoma cells and make the cells more susceptible to 5-deoxy-5-fluorouridine. Jpn J Cancer Res 84: 341-347 1993 https://doi.org/10.1111/j.1349-7006.1993.tb02876.x
  25. Cheng GC, Lee JY, Kim DC, Sub SO, Hwang WI. Inhibitory Effect of Salvia miltiorrhiza Extract on Growth of Some Cancer Cells. J Kor Soc Food Sci Nutr 29(4): 726-731, 2000
  26. Tracy KU, Cerami A. Tumor Necrosis factor: A pleiotropic cytokine and therapeutic target. Annu Rev Med 45: 491-503, 1994 https://doi.org/10.1146/annurev.med.45.1.491
  27. Cederholm T, Wretlined B, Hellstrom K, Andersson B, Engstrom L, Brismar K, Scheynius A, Forshid J, Palmblad J. Enhanced generation of interleukin I beta and 6 may contribute to the cachexia of chronic disease. Am J Clin Nutr 65: 876-882, 1997
  28. Ydh S, Schuster MW. Geriatric cachexia: the role of cytokine. A J Clin Nutr 70(2): 183-197, 1999
  29. Kawamura I, Lacey E, Yamamoto N, Sakai T, Takeshita S, Inami M, Nishigaki F, Naoe Y, Tsujimoto S, Manda T, Shimomura K, Goto T. Ponalrestat. an aldose reductase inhibitor, inhibits cachexia syndrome induced by colon26 adenocarcinoma in mice. Anticancer Res 19: 4105-4112, 1999
  30. Yamamoto N, Kawamura I, Nishigaki F, Tsujimoto S, Naoe Y, Inami M, Elizabeth L, Manda T, Shimomura K. Effect of FR-143430, a Novel Cytokine Suppressive Agent, on Adenocarcinoma Colon26-Induced Cachexia in Mice. Anticancer Research 18: 139-144, 1998
  31. RHee YK, Han MJ, Park SY, Kim DH. In vitro and in vivo antitumor activity of the fruit body of phellinus linteus. J Kor Food Sci Technol 32(2): 477-480, 2000