Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characteristics of Nitrobenzene Degradation by Mycobacterium chelonae Strain NB01

  • Oh, Young-Sook (Department of Environmental Engineering & Biotechnology, Myongji University) ;
  • Lee, Youn-Hee (Division of Life Sciences, Hallym University) ;
  • Lee, Jung-Hyun (Microbiology Lab., Korea Ocean Researc & Development Institute) ;
  • Choi, Sung-Chan (Division of Life Sciences, Hallym University)
  • Published : 2003.04.01
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Abstract

A bacterial strain NB01, isolated from wastewater, was found to utilize nitrobenzene (NB) as the sole source of nitrogen, carbon, and energy. The strain was classified as a member of a high G+C Gram-positive group and identified as Mycobacterium chelonae based on an analysis of its 16S rRNA gene sequence. The strain grew on NB with a concomitant release of about 63% of the total available nitrogen as ammonia, suggesting a reductive degradation mechanism. The optimal pH and temperature for degradation were PH 7.0-8.0 and $30^{\circ}C$, respectively. The cell growth was retarded at NB concentrations above 1.8 mM. The degradation of NB followed Michaelis-Menten kinetics within the tolerance range, and the $K_m$ and maximum specific removal rate for NB were 0.33 mM and $11.04\;h^{-1}$, respectively.

Keywords

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