Journal of Microbiology and Biotechnology

  • 제13권2호
  • /
  • Pages.207-216
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  • 2003
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Isolation and Identification of a Lactic Acid Bacterial Strain KJ-108 and Its Capability for Deodorizing Malodorous Gases Under Anaerobic Culture Conditions

  • KIM, JEONG-DONG (Department of Life Science, Hanyang University) ;
  • JUNG-HOON YOON (Korea Research Institute of Bioscience and Biotechnology) ;
  • YONG-HA PARK (Korea Research Institute of Bioscience and Biotechnology) ;
  • DAE-WEON LEE (The Institute of Life Science and Technology, Sungkyunkwan University) ;
  • KYOU-SEUNG LEE (The Institute of Life Science and Technology, Sungkyunkwan University) ;
  • CHANG-HYUN CHOI (The Institute of Life Science and Technology, Sungkyunkwan University) ;
  • WON-YEOP PARK (The Institute of Life Science and Technology, Sungkyunkwan University) ;
  • KOOK-HEE KANG (The Institute of Life Science and Technology, Sungkyunkwan University)
  • 발행 : 2003.04.01
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초록

A number of different sources, such as composts, leachates, and pig feces samples, were collected from different pig farms in Korea, and several microorganisms were screened for their ability to deodorize the malodorous gases. Consequently, a novel malodorous gases-deodorizing bacterial strain, KJ-108. was isolated, because it was highly abundant in nitrate-supplemented minimal medium ($MM-NO_3^-$) under anaerobic culture conditions. Airtight crimp-sealed serum bottles containing $MM-NO_3^-$ , medium were inoculated with KJ-108. Nitrate concentration was decreased rapidly after 20 h of incubation, and incubation was carried out until nitrite production reached almost zero. Taxonomic identification, including 16S rDNA base sequencing and phylogenetic analysis, indicated that the isolate had $100\%$ homology in its 165 rDNA base sequence with Lactobacillus pentosus. Among the volatile fatty acids, acetic acid contained in large amounts in fresh piggery slurry was decreased by about $40\%$ after 50 h incubation with strain KJ-108. n-Butyric acid, n-valeric acid, and isovaleric acid were gradually decreased, and isobutyric acid and capronic acid were dramatically eliminated at theinitial period with the treatment. Moreover, NH, removal efficiency reached a maximum of $98.5\%$ after 50 h of incubation, but the concentration of $H_2S$ was not changed.

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참고문헌

  1. Standard Methods for Examination of Water and Wastewater (19th Ed.) APHA;A.D. Eaton(ed.);L. S. Clesceri(ed.);A. E. Greenberg(ed.)
  2. J. Microbiol. Biotechnol. v.2 Utilization of deodorized poultry feces with Tolura sp. CH-30 Choi, M. Y.;E. Lee
  3. Environ. Prog. v.17 Biotreatment of ammonia in air by an immobilized nitrosomonas europaes biofilter Chung, Y. C.;C. Huang https://doi.org/10.1002/ep.670170211
  4. J. Biotechol. v.52 Operation optimization of Thiobacillus thioparus CH11 biofilter for hydrogen sulfide removal Chung, Y. C.;C. Huang;C. P. Tseng https://doi.org/10.1016/S0168-1656(96)01622-7
  5. Biotechnol. Prog. v.13 Biotreatment of ammonia from air by an immobilized Arthrobacter oxydans CH8 biofilter Chung, Y. C.;C. Huang;C. P. Tseng https://doi.org/10.1021/bp970065e
  6. Chemosphere v.43 Biological elimination of $H_2S and NH_3$ from wastegases by biofilter packed with immobilized heterobavtrophic bacteria Chung, Y. C.;C. Huang;C. P. Tseng https://doi.org/10.1016/S0045-6535(00)00211-3
  7. Odor Preservation and Control of Organic Sludge and Livestock Farming Eikum, A. S.;R. Storhang
  8. PHYLIP:Phylogenetic Inference Package. Felsenstein, J.
  9. Methods for General and Molecular Bacteriology Gerhardt, P.;R. G. F. Murray;W. A. Wood;N. R. Krieg
  10. Biological Reclamation of Solid Waste Golueke, C. G.
  11. J. Water Pollut. Control v.52 Odor control; an operators guide Henry, J. G.;R. Gehr
  12. Vibrio algionolyticus. Biotechnol. Lett. v.22 Removal characteristics of high ammonia gas by a biofilter seeded with a marine bacterium Kim, N. J.;Y. Sugano;M. Hirai;M. Shoda https://doi.org/10.1023/A:1005605706932
  13. J. Microbiol. Biotechnol. v.3 Removal of inorganic nitrogen and phosphorus from cows liquid manure by batch culture Kim, S. H.;M. Y. Pack
  14. J. Microbiol. Biotechnol. v.10 Simultaneous removal of hydroen sultide and ammonia using Thiobacillus sp. IW in a three-phase fluidized -bed bioreactor Kim, S. H.;K. J. Oh;J. H. Moon;D. Kim
  15. J. Microbiol. Biotechnol. v.11 Application thermophilic aerobic digestion process to industrial waste activated sludge treatment Kim, Y. K.;Y. S. Eom;B. K. Oh;W. H. Lee;J. W. Choi
  16. J. Microbiol. Biotechnol. v.11 Removal behavior of biological nitrogen and phosphorous, and prediction of microbial community composition with its function, in an anaerobicanoxic system from weak sewage Lee, J. W.;E. S. Choi;K. I. Gil;H. W. Lee;S. H. Lee;S. Y. Lee; Y. K. Park
  17. J. Air Waste Management Assoc v.41 Biofiltration:An innovative air pollution control technology for VOC emission Lesson, G.;A. M. Winer https://doi.org/10.1080/10473289.1991.10466898
  18. Methods for General and Molecular Bacteriology Determinative and cytological light microscopy Murry, R. G. E.;N. R. Doetsch;C. F. Robinow;P. Gerhardt(ed.)
  19. Bioresour. Technol. v.72 Physical removal of textile dyes and silidstate fermentation of dye-adsorbed agricultural residues Nigam, P.;G. Armour;I. M. Banat;D. Singh;R. Marchant https://doi.org/10.1016/S0960-8524(99)00123-6
  20. J. Mictobiol. Biotechnol. v.11 Bioelectrochemical denitrification by Pseudomonas sp. or anaerobic bacteria consortium Park, D. H.;Y. K. Park
  21. Adv. Microbiol. Physiol. v.30 Autotrophic nitrification in bacteria Prosser, J.L.
  22. Plant/Oper. Prog. v.10 Health effects of ammonia Ryer-Power, J. E. https://doi.org/10.1002/prsb.720100411
  23. Mol. Biol. Evol. v.4 The neighbor-joining method: A new method for reconstructing phylogenetic trees Saitou, N.;M. Nei
  24. Handbook of New Bacterial Systematic Nucleic acids and classification Stackebrandt, E.;W. Liesack;M. Goodfellow(ed.);A. G. O. Donnell(ed,)
  25. Biology of Anaerobiv Microorganisms Dissimulator reduction of oxifized nitrogen compounds Stouthamer, A. H.;A. J. B. Zehnder(ed.)
  26. J. Appl. Microbiol. v.90 Comparisons of aerobic denitrification under high oxygen atmosphere by Thiophaera pantotropha ATCC 35512 and Pseudomonas stuzeri SU2 newly isolated from the activated sludge of a piggery wastewater treatment system Su, J. J.;B. Y. Liu;C. Y. Liu https://doi.org/10.1046/j.1365-2672.2001.01265.x
  27. J. Air Waste Management Assoc v.44 Biofiltration control of hydrogen sulfide design and operation parameters Yang, Y.;E. R. Allen
  28. Int. J. Syst. Bacterial v.47 Restriction fragment length polymorphisms analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains Yoon, J. H.;S. T. Lee;S. B. Kim;W. Y. Kim;M. Goodfellow;Y. H. Park https://doi.org/10.1099/00207713-47-1-111
  29. The Nitrogen and Sulphur Cycles Biocheminal and physiological aspects of denitrification Zumft, W. G.;A. Viebroc;H. Koerner;J. A. Cole(ed.);S.J. Ferguson(ed.)