Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Isolation of Gamma-Induced Rice Mutants with Increased Tolerance to Salt by Anther Culture

  • 발행 : 2003.03.01
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초록

Doubled haploids have long been recognized as a valuable tool in plant breeding since it not only offers the quickest method of advancing heterozygous breeding lines to homozygosity, but also increased the selection efficiency over conventional procedures due to better discrimination between genotypes within any one generation. Salt tolerant mutants were obtained in rice the variety, 'Hawsungbyeo', through in vitro mutagenesis of in vitro cultured anther-derived calli. Various doses (30, 50, 70 and 90 Gy) of gamma ray were applied to investigate the effect of radiation on callus formation on medium containing 1% NaCl, green plant regeneration, frequency of selected doubled haploid mutants and of the salt tolerant screen. It was demonstrated that the dose of 30 and 50 Gy gamma rays had significant effects on callus formation, regeneration and selection of salt tolerance. No tolerant lines were obtained from non-mutagenized cultures. From gamma ray irradiated cultures, five tolerant lines ($M_2$generation) at germination stage and 13 tolerant lines ($M_3$genoration) at seedling stage were obtained. The frequency of salt tolerant mutants indicates that anther culture applied in connection with gamma rays is an effective way to improve salt tolerance.

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참고문헌

  1. The impact of Biotechnology in Agriculture. In vitro radiation induced mutagenesis in potato. In: Sangwan RS;Sangwan Noreel BS, (Eds) Ahloowalia BS
  2. Euphytica v.101 Mutation breeding of highgate for tolerance to Fursarium oxysporum f. sp. cubense using gamma irradiation. Bhagwat B;Duncan EJ https://doi.org/10.1023/A:1018391619986
  3. Science v.218 Plant productivity and environment. Boyer JS https://doi.org/10.1126/science.218.4571.443
  4. Plant Cell v.2 Characterization of a rice gene showing orgen-specific expression in response to salt stress and drought. Claes B;Dekeyser R;Vinaroel R;Vanden Bulcke M;Bauw G;Van Montagu M;Caplan A https://doi.org/10.1105/tpc.2.1.19
  5. Radiation Biology. Effects of radiation on the cell. In: Casarett AP,(Eds) Casarett AP
  6. Acta Agri Nucl Sinica v.11 Effects of gamma irradiation and other factors on the inductionof calli and regeneration of green plants in rice anther culture. Chen QF;Afza R;van Duren M;Brunner H
  7. Euphytica v.120 Anther culture in connection with induced mutations for rice improvement. Chen QF;Wang CL;Lu YM;Shen M;Afza R;van Duren M;Brunner H https://doi.org/10.1023/A:1017518702176
  8. Sci Sinica v.18 Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Chu CC;Wang CC;Dun CS;Hsu C;Yin KC;Chu Y;Bi FI
  9. Theor Appl Genet v.55 An effective chemical nutagenesis procedure for Petunia hydrida cell suspension cultures. Colijn CM;Kool AJ;Nijkamp https://doi.org/10.1007/BF00295434
  10. Annu Prog La Agric Exp Stn v.52 The use of anther culture to expedite the breeding and release of new varieties of rice. Croughan TP;Chu QR;McKenzie KS;Huang HJ;Meche MM
  11. Euphytica v.114 Induced of mutations for heat tolelance in potato by using in vitro culture and radiation. Das A;Gosal SS;Sidhu JS;Dhaliwal HS https://doi.org/10.1023/A:1003965724880
  12. Plant Physiol v.81 Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Hurkman WJ;Tanaka CK https://doi.org/10.1104/pp.81.3.802
  13. Los Banos IRRI
  14. Nature v.227 Cleavage of structural protein during the assembly of the head of bacteriophage T4. Laemmli UK https://doi.org/10.1038/227680a0
  15. Acta Agri Zhejiangensis v.9 Effect of γ-irradiation on the formation of calli and regeneration of green plants in rice anther culture. Lu YM;Wang CL;Shen M;Chen QF
  16. J Exp Bot v.46 Changes in plant response to NaCl during development of rice varieties differing in salinity resistance. Lutts S;Kinet JM;Bouharmont J https://doi.org/10.1093/jxb/46.12.1843
  17. Mol Gen Genet v.196 Ultraviolet mutagenesis and genetic analysis of resistance to methylglyoxal-bis (guanyhydrazone) in tobacco. Malmberg RL;Mcindo J https://doi.org/10.1007/BF00334088
  18. Euphytica v.85 Application of in vivo and in vitro mutation techniques for crop improvement. Maluszynski M;Ahloowalia BS;Sigubjornsson B https://doi.org/10.1007/BF00023960
  19. J lrrig Drainage Div ASCE v.103 Crop salt tolerance-current assessment. Mass EV;Hoffmann GJ
  20. Euphytica v.91 Production of salt tolerant rice breeding line via doubled haploid. Miah MAA;Pathan MS;Quayum HA https://doi.org/10.1007/BF00033089
  21. Plant Cell v.9 Antagonistic effects of ABA and Jasmonates on salt stress-inducible transcripts in rice roots. Moons A;Prinsen E;Bauw G;Montagu MV https://doi.org/10.1105/tpc.9.12.2243
  22. In vitro mutation system for crop improvement. In: Plant mutation Breeding for Crop Improvement. v.2 Novak FJ
  23. J Bio Chem v.250 High resolution two-dimensional electrophoresis of proteins. O' Farrell PH
  24. Agricell Report v.19 Gamma rays stimulate growth, regeneration and product synthesis in plant tissue cultures. Onisei T;Toth E;Amariei D;Balini S
  25. Plant Cell Tissue and Organ Culture v.70 Selection of rice for iron toxicity tolerance through irradiated caryopsis culture. Pathirana R;Wijithawarna WA;Jagoda K;Ranawaka AL https://doi.org/10.1023/A:1016025728740
  26. Agron J v.51 Salinity effects at several growth stages. Pearson GA;Bernstein L https://doi.org/10.2134/agronj1959.00021962005100110007x
  27. Prod Res Rep v.43 Rice as a crop for salt for saltaffected soil in process of reclamation. Pearson GA;Ayers AD
  28. Theor Appl Genet v.95 Establishment of stable NaCI resistant rice plant lines from anther culture: distribution pattern of $K^+/Na^+$ in callus and plant cells. Sathish P;Gamborg OL;Nabores MW https://doi.org/10.1007/s001220050682
  29. Current Options for Cereal Improvement Induction of salt tolerance in high yielding rice yielding rice varieties through mutagenesis and anther culture. In: M. Maluszynski (Ed.) Zapata FJ;Aldemita RR