Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

In Vitro Propagation of Zantedeschia spp. through Shoot Tip Culture

경정배양에 의한 Zantedeschia spp.의 기내번식

  • Published : 2003.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

This experiment was conducted to propagate Zantedeschia spp. in vitro. The frequency of adventitious bud clusters (ABC) formation from shoot tips in Z. 'Best Gold' was high at more than 65% on media with 2.0∼5.0 mg/L BA or 0.1∼1.0 mg/L thidiazuron. The highest formation rate of ABC (75%) was obtained on medium containing 2.0 mg/L BA. Comparing to treatment of BA alone, combined one of BA and NAA did not stimulate the formation of ABC and the shoot regeneration from shoot tips. The proliferation of ABC from sections (0.7∼1.0 cm) of ABC occurred effective on medium with 2.0 mg/L BA. Shoots developed from the sections (0.7∼1.0 cm) of ABC grew and rooted favorably on media containing 1.0∼2,0 mg/L IBA. The shoots were multiplicated effectively on medium with 0.5 mg/L thidiazuron in Z. 'Childsiana', on medium with 3.0 mg/L BA in 2. 'Golden Affair', and on medium with 5.0∼10.0 mg/L BA in Z. 'Pacific Pink'.

본 실험은 경정에서 다아체 형성을 통하여 Zantedeschia의 기내 대량번식 체계를 확립하기 위하여 실시하였다. Z. 'Best Gold'의 경정에서 다아체 형성률은 BA 2.0∼5.0 mg/L 및 TDZ 0.1∼l.0mg/L가 첨가된 배지에서 63% 이상으로 높았으며, BA 2.0 mg/L가 첨가된 배지에서는 75%로 가장 높았다. BA와 NAA의 혼용첨가는 BA 단용첨가배지와 비교하여 Z. 'Best Gold'의 신초경정에서 다아체의 형성 및 신초분화를 촉진하지 않았다. Z. 'Best Gold'의 형성된 다아체 절편체 (0.7∼1.0 cm)에서 다아체의 증식은 BA 2.0mg/L를 첨가한 배지가 효과적이었다. 형성된 다아체 절편체 (0.7∼l.0 cm)에서 신초의 생육 및 발근은 IBA 1.0∼2.0 mg/L가 첨가된 배지가 적합하였다. Z. 'Childsiana'는 TDZ 0.5 mg/L가 첨가된 배지에서, Z. 'Golden Affair'는 BA 3.0 mg/L 첨가배지가, Z. 'Pacific Pink'는 BA 5.0∼10.0 mg/L가 첨가된 배지에서 신초증식이 양호하였다.

Keywords

References

  1. Cohen D (1981) Micropropagation of Zantedeschia hybrids. Comb Proc Intl Plant Prop Soc 31: 312-316
  2. Corr BE, Widmer RE (1991) Paclobutrazol, gibberellic acid, and rhizome size affect growth and flowering of Zantedeschia. HortSci 22: 133-135
  3. Earle ED, Langhans RW (1974) Propagation of Chrysanthemum in vitro. I. Multiple plantlet fron shoot tip and the establishment of tissue culture. J Amer Soc Hort Sci 99: 128-132
  4. Fellman CD, Read PE, Hosier MA (1987) Effect of thidiazuron and CPPU on meristem formation and shoot proliferation. HortSci 22: 1197-1200
  5. Fiola JA, Hassan MA, Swalz RH, McNicols R (1990) Effect of the thidiazuron, light influence rates and kanamycin on in vitro organogenesis from excised Rubus cotylendons and leaves. Plant Cell Tiss Org Cult 20: 223-228
  6. Funnell KA (1993) Zantedeschia. The physiology of flower bulbs. 36: 683-704
  7. Han BH, Yae BW, Goo DH, Ko JY (1999) Effects of growth regulators and light on the formation and proliferation of bulblets with swollen basal plate from in vitro culture of bulbscales in Lilium oriental hybrid 'Casa Blanca'. J Kor Soc Hort Sci 40: 463-466
  8. Han BH, Yae BW, Yu HJ, Shin JS (2001) In vitro propagation of Spathiphyllum floribundum cv. Cupid. Kor J Plant Tissue Cult 28: 209-213
  9. Hartman RD (1974) Dasheen mosaic virus and other phytopatho-gens eleminated from caladium, taro, and cocoyam by culture of shoot tips. Phytopathology 64: 237-240 https://doi.org/10.1094/Phyto-64-237
  10. Kusey WE, Hammer PA, Weiler TC (1980) In vitro propagation of Gypsophila paniculata L. 'Bristol Fairy' Hort Sci 15: 600-601
  11. Lee YS (1996) Micropropagation by apical meristem culture of colored Calla lily (Zantedeschia spp.) and effects on the bulb development of nutriculture of tissue-cultured plantlets. MS thesis, Chonbuk National Univ
  12. Malik KA, Saxena PK (1992) Regeneration in Phaseolus vulgaris L.; High-frequency induction of direct shoot formation in intact seedling by $N^6$-benzylaminopurine and thidiazuron. Planta 186: 384-389
  13. Murashige T, Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue culture. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  14. Reynolds JF (1987) Chemical regulation in tissue culture: An overview. Hort Sci 22: 1192-1194
  15. Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation. Sym Soc Exp Biol 11: 118-131
  16. Takayama S, Misawa M(1982) Regulation of organ formation by cytokinin and auxin in Lilium bulbscales grown in vitro. Plant Cell Physiol 23:67-74
  17. Tjia B (1987) Growth regtulator effect on growth and flowering of Zantedeschia rehmannii hybrid. Hort Sci 22:507-508
  18. Yu KJ, Peak KY (1995) Micropropagation of Anthurium spp. through shoot tip and callus culture. J Kor Soc Hort Sci 36: 684-694