Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Characterization of the Immunologically Active Components of Glycyrrhiza uralensis Prepared as Herbal Kimchi

  • Hwang, Jong-Hyun (Department of Kimchi and Food Science, Chongju National College of Science and Technology) ;
  • Lee, Kyong-Haeng (Department of Kimchi and Food Science, Chongju National College of Science and Technology) ;
  • Yu, Kwang-Won (Department of Kimchi and Food Science, Chongju National College of Science and Technology)
  • Published : 2003.03.01
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Abstract

A crude polysaccharide fraction (GU-3) from the roots of Glycyrrhiza uralensis (licorice root), a screened herbal plant used in the preparation of herbal kimchi, enhanced Peyer's patch mediated bone marrow cell proliferation and NK cell-mediated tumor cytotoxicity against Yac-1 cells. GU-3 was further purified by DEAE-Sepharose CL-6B yielding fractions designated as GU-3I, and 3IIa∼3IIe. GU-3IIa is mainly composed of arabinose, galactose and galacturonic acid, and showed the highest bone marrow cell proliferation activity. In addition, GU-3IIb had arabinose, galactose, rhamnose and galacturonic acid as the component sugars with a small quantity of protein; GU-3IIb also enhanced activity of NK cell-mediated tumor cytotoxicity. After these fractions were further fractionated via gel filtration on Sepharose CL-6B or Sephacryl S-300, two immunological active polysaccharides, GU-3IIa-2 and 3IIb-1 were purified from the respective fractions. GU-3IIa-2 mostly contained neutral sugars (75%) such as arabinose and galactose (molar ratio; 1.0 : 0.7) in addition to a considerable amount of galacturonic acid (20%), whereas GU-3IIb-1 was composed of arabinose, galactose, rhamnose and galacturonic acid (molar ratio; 0.3 : 0.5 : 0.1 : 1.0). Methylation analysis indicated that GU-3IIa-2 was composed mainly of terminal, 4- or 5-linked and 3,4- or 3,5-branched arabinose, 3-linked, 4-linked and 3,6-branched galactose, and terminal and 4-linked galacturonic acid whereas GU-3IIb-1 contained various glycosidic linkages such as terminal and 4- or 5-linked arabinose, 2,4-branched rhamnose, terminal and 4-linked galactose, and terminal and 4-galacturonic arid. Single radial gel diffusion indicated that only GU-3IIa-2 strongly reacted with β-D-glucosyl-Yariv antigen. These results suggest that bone marrow cell proliferating activity and enhancement of NK cell-mediated tumor cytotoxicity of GU-3 are caused by polysaccharides containing a pectic arabinogalactan (GU-3IIa-2) and pectic polysaccharide (GU-3IIb-1).

Keywords

References

  1. Yamada H, Kiyohara H. 1989. Bioactive polysaccharides from Chinese herbal medicines. In Abstracts of Chinese Medicines (ACMA). Chang HM, ed. The Chinese University of Hong Kong, N. T. Shatin: Chinese Medicinal MaterialResearch Center. Vol 3, p 104-124
  2. Dey PM, Brinson K. 1984. Plant cell walls. In Advances Carbohydrate Chemistry and Biochemistry. Academic Press, New York, USA. p 265-382
  3. Yamada H. 1994. Pectic polysaccharides from Chinese herbs: Structure and biological activity. Carbohyd Polym 25: 269-276 https://doi.org/10.1016/0144-8617(94)90052-3
  4. Fujiwara Y, Kikkawa R, Nakata K, Kitamura E, Takama T, Shigeta Y. 1983. Hypokalemia and sodium retention in patients with diabetes and chronic hepatitis receiving in-sulin and glycyrrhizin. Endocrinol Jpn 32: 243-249
  5. Baschetti R. 1995. Chronic fatigue syndrome and liquorice. N Z Med J 108: 156-157
  6. Okimasu E, Moromizato Y, Watanabe S, Sasaki J, Shiraishi N, Morimoto YM, Miyahara M, Utsumi K. 1983. Inhi-bition of phospholipase A2 and platelet aggregation by glycyrrhizin, an antiintlammation drug. Acta Med Okayama 37: 385-391
  7. Yoshikawa M, Matsui Y, Kawamoto H, Umemoto N, Oku K, Koizumi M, Yamao J, Kuriyama S, Nakano H, Hozumi N, Ishizaka S, Fukui H. 1997. Effects of glycyrrhizin on immune-mediated cytotoxicity. J Gastroenterol Hepatol 12: 243-248 https://doi.org/10.1111/j.1440-1746.1997.tb00416.x
  8. O'Brian CA, Ward NE, Vogel VG. 1990. Inhibition of protein kinase C by the 12-O-tetradecanoylphorbol-13- acetate antagonist glycyrrhetic acid. Cancer Lett 49: 9-12 https://doi.org/10.1016/0304-3835(90)90132-H
  9. Lee KH, Hwang JH, Yu KW. 2003. Immunological com-ponents from Glycyrrhiza uralensis prepared as herbal kimchi. Food Sci Biotechnol submitted
  10. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350-356 https://doi.org/10.1021/ac60111a017
  11. Blumenkrantz N, Asboe-Hansen G. 1973. New method for quantitative determination of uronic acid. Anal Biochem 54: 484-489 https://doi.org/10.1016/0003-2697(73)90377-1
  12. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  13. Johnes TM, Albersheim P. 1972. A gas chromatographic method for the determination of aldose and uronic acid constituents of plant cell wall polysaccharides. Plant Physiol 49: 926-936 https://doi.org/10.1104/pp.49.6.926
  14. Zhao JF, Kiyohara H, Yamada H, Takemoto N, Kawamura H. 1991. Heterogeneity and characterization of mitogenic and anti-complementary pectic polysaccharides from the roots of Glvcyrrhiza uralensis Fisch et DC. Carbohyd Res 219: 149-172 https://doi.org/10.1016/0008-6215(91)89049-L
  15. van Holst GJ, Clarke AE. 1985. Quantification of arabino-galactan-protein in plant extracts by single radial gel dif-fusion. Anal Biochem 148: 446-450. https://doi.org/10.1016/0003-2697(85)90251-9
  16. Hakomori S. 1964. A rapid permethylation of glycolipid, and polysaccharide catalyzed by methylsulfinyl carbanion in dimethyl sulfoxide. J Biochem (Tokyo) 55: 205-208
  17. Waeghe TJ, Darvill AG, McNeil M, Albersheim P. 1983. Determination by methylation analysis of the glycosyl lin-kage compositions of microgram quantities of complex carbohydrates. Carbohyd Res 123: 281-304 https://doi.org/10.1016/0008-6215(83)88484-5
  18. York WS, Darvill AG, McNeil M, Stevenson TT, Albersheim P. 1986. Isolation and characterization of plant cell walls and cell wall components. Methods Enzymol 118: 3-40 https://doi.org/10.1016/0076-6879(86)18062-1
  19. Sweet DP, Shapiro RH, Albersheim P. 1975. Quantitative analysis by various GLC. response-factor theories for par-tially methylated and partially ethylated alditol acetates. Carbohvd Res 40: 217-225 https://doi.org/10.1016/S0008-6215(00)82604-X
  20. Hong T, Matsumoto T, Kiyohara H, Yamada H. 1998. Enhanced production of hematopoeitic growth factors through T cell activation in Peyer's patches by oral administration of Kampo (Japanese herbal) medicine 'Juzen-Taiho-To', Phytomedicine 5: 353-360 https://doi.org/10.1016/S0944-7113(98)80017-2
  21. Page B, Page M, Noel C. 1993. A new fluorometric assay for cytotoxicity measurements in vitro. lnt J Oncol 3: 473-476
  22. Yoo YC, Watanabe S, Watanabe R, Hata K, Shimazaki K, Azuma I. 1997. Bovine lactoferrin and lactoferricin, a pep-tide derived from bovine lactoferrin, inhibit tumor metas-tasis in mice. Jpn J Cancer Res 88: 184-190 https://doi.org/10.1111/j.1349-7006.1997.tb00364.x
  23. Clarke AE, Anderson RL, Stone BA. 1979. Form and function of arabinogalactans and arabinogalactan-proteins. Phytochemistry 18: 521-540 https://doi.org/10.1016/S0031-9422(00)84255-7
  24. Yu KW, Hwang JH. 2001. Characterization of bone marrow cell proliferating arabinogalactan through Peyer's patch cells from rhizomes of Atractylodes lancea DC. J Food Sci Nutr 6: 180-186
  25. Kiyohara H, Yamada H. 1989. Structure of an anti-com-plementary arabinogalactan from the root of Angelica ac-utiloba Kitagawa. Carbohyd Res 193: 173-192 https://doi.org/10.1016/0008-6215(89)85117-1