Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
권호 표지

A Study on Cytokines in the Mongolia Mare's Milk

몽고 마유에 함유된 사이토카인에 관한 연구

  • Published : 2003.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to detect the pro-inflammatory cytokines(IL-1, IL-6, TNF-a, IL-18) and IL-1 receptor accessory in mongolia mare's milk by western blotting. IL-1 and TNF-a were detected in 4 samples of mare's milk Proteins of 6 kD and 7 kD were bound to specific antibody against hIL-18. But, IL-l and TNF-a were not detectable in Difco skim milk IL-6 like factor of 60 kD was detected in both Difco skim milk and mare's milk. Also, IL-1 receptor accessory of 55 kD was detected in the mongolia mare's milk.

본 연구는 몽고 마유에서 pro-inflammatory cytokine(IL-l$\beta$, IL-6, TNF-a, IL-18)과 IL-1 receptor accessory를 Western blotting방법으로 확인하여 기능성 식품과 의약품 소재로 이 용하기 위한 기초 자료를 얻고자 실시하였다. 몽고 마유의 4개군 모두에서 IL-1, TNF-a가 17 kD에서 확인되었다. IL-18은 6~7 kD에서 확인할 수 있었다. 그리고, IL-1 receptor accessory를 55 kD에서 확인하였다. 그러나 탈지분유(Difco) 에서는 IL-l$\beta$, TNF-a를 확인할 수 없었으나, IL-6는 탈지분유(Difco) 및 몽고 마유에서 모두 60 kD에서 확인하였다. 이 와 같이 몽고 마유는 다양한 cytokine을 함유하고 있어서 기능성 식품 및 의약품 소재로 이용가치가 있다고 사료된다.

Keywords

References

  1. Bell, K., Mckenzie, H. A., Muller, V., and Rogers, C. (1981) Equine whey proteins. Comp. Biochem. Physiol. 68B, 225-231
  2. Bumette, W. H. (1981) Westem blotting. Anal. Biochem. 112,195-202 https://doi.org/10.1016/0003-2697(81)90281-5
  3. Cogourdant, B. and Drogoul, C. (1988) Projetde I'EA RL des Monts Feuillots Met tre enplace une jumenterie laitier avec commercialisation de produi-ts a base de lait de jurnent: lait de jument fermente et produits cosmetiques', Corcelles les Monts,Dijon, France. 123-129
  4. Doreau, M. and Boulot, S. (1989) Methods of measurement of milk yield and composition in nursing mares. Lait. 69, 159-166 https://doi.org/10.1051/lait:1989313
  5. Drogoul, C. and Monotenot, C. (1990) Document d'information CE, Direction Generale VI, n. FRA1201348, 'Diversification des productions agriroles par la valoris ation du lait et du colostrum des juments de races lourdes dans le but de sau vegarder ces races de chevaux', PP.63-90
  6. Kalliala, H., Seleste, E., and Hallman, N. (1951) On the use of mare's milk in infant feeding. Actci Pediatrica 40, 94-102 https://doi.org/10.1111/j.1651-2227.1951.tb15789.x
  7. Katsuro, H., Satoshi, K.., Hitoki, Y., Rikio, K., and Hiroshi, I (2000) Detection of cytokines in bovine colostmm. Ver. Immunol. 76, 183-190 https://doi.org/10.1016/S0165-2427(00)00213-0
  8. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacterio phage T4. Nature 227, 680-688 https://doi.org/10.1038/227680a0
  9. Nassal, J. and Rembalski, C. (1980) Hygienische Forderungen bei der Produktion von Stutenmilk und Kumy$\beta$. Archiv fur Lehen-smitteIhygiene 31(6), 209-216
  10. Pitruzzella, E. (1990) "'L'Alimentazionenel bambino Sano e Malato"1st ed., Medical books. Palermo, Italy, PP. 131-140
  11. Rudloff, H. E., Schmalstieg, F. C., Mushtaha, A. A., Palkowetz, K. H., Liu, S. K., and Goldman, A. S. (1992) Tumor necrosis factor-alpha (TNF-a) in human milk. Pediatr. Res. 31, 29-33 https://doi.org/10.1203/00006450-199201000-00005
  12. Saito, S., Maruyama, M., Kato, Y., Moriyama, I., and Ichijo, M (1991) Detection of IL-6 in human milk and its involvement in IgA production. J. Reprod ImmunoI. 20, 267-276 https://doi.org/10.1016/0165-0378(91)90051-Q
  13. Servetnik-Chalaya, G., and Maltseva, L. (1981) Characteristics of the vitamin composition of mare's milk and koumiss depending on the season. Vopr. Pitan. 4, 46-53
  14. Soder, O. (1987) Isolation of interleukin-l from human milk. J. AppI. Immunot. 83, 19-23
  15. Solaroli, G. (1992) Caratterizzazione chimicofisica e valutazione della resistenza termica del latte di cavalla. Ph.D. Thesis, Univ. of Milan, Italy, PP. 13-27
  16. Towin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. 76, 4350-4356 https://doi.org/10.1073/pnas.76.9.4350
  17. Welsch, D., Buchheim, W., Schumacher, U., Schinko, I., and Patton, S. (1988) Structural histochemical and biochemical obser-vation on horse milk fat globule membranes and casein micelles .Histochemistry 88, 357-365
  18. Yasushi, T., Hidetoshi, T., Akihiko, N., Koichi, 0., Hideki, N.,Tomoteru, T., Hito, N., and Toshiro, H. (2001) Interleukin-lS in human milk. Pediatric Res. 50, 268-272 https://doi.org/10.1203/00006450-200108000-00017
  19. Yoon, D. Y. and Dinarello, C. A. (1998) Antibodies to domain II and III ofthe IL-1R accessory protein inhibit IL-1$\beta$ activity but notbinding: Regulation of IL-l responses is via IL-1RI but not IL-lRAcP. J. Immunol. 160, 3170-3179