International Journal of Industrial Entomology and Biomaterials

  • 제6권1호
  • /
  • Pages.81-85
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  • 2003
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  • 3022-7542(pISSN)
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  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
권호 표지

Spacing Effect of the Intervening Sequences between Ribosome Binding Site and the Initiation Codon on Expression of Bacillus thuringiensis $\delta$-Endotoxin

  • Roh, Jong-Yul (School of Agricultural Biotechnology, Seoul National University) ;
  • Li, Ming-Shun (School of Agricultural Biotechnology, Seoul National University) ;
  • Chang, Jin-Hee (School of Agricultural Biotechnology, Seoul National University) ;
  • Park, Jae-Young (School of Agricultural Biotechnology, Seoul National University) ;
  • Shim, Hee-Jin (School of Agricultural Biotechnology, Seoul National University) ;
  • Woo, Soo-Dong (Takara Korea Biomedical Inc) ;
  • Boo, Kyung-Saeng (School of Agricultural Biotechnology, Seoul National University) ;
  • Je, Yeon-Ho (School of Agricultural Biotechnology, Seoul National University)
  • 발행 : 2003.03.01
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초록

To verify importance of the intervening sequence between the ribosome binding site (RBS) and the initiation codon for expression of Bacillus thuringiensis $\delta$-endotoxin, the pProMu, containing SphI and NcoIsites between RBS and the initiation codon of the cry1Ac gene, and the deletion derivatives of pProMu were constructed and transformed into the B. thuringiensis subsp. kurstaki $Cry^{-B}$ strain. The pProMu-ΔSphIhad identical six bases of intervening sequence to pProAc though the arrangement of sequence was different. Other mutants containing pProMu had 1 or 10 or 14 bases between RBS and the initiation codon. Among deletion mutants, only ProMu-ΔSphI/CB only produced 130 kDa typical bipyramidal crystals like those seen for ProAc/CB. However, ProMu/CB, $ProMu-{\Delta}NcoI$, and ProMu-ΔSphI＋NcoIdid not produce Cry1Ac crystals. In conclusion, the results suggest that 6-base intervening sequence was important for expression of cry1-type class gene. Furthermore, spacing effect of the intervening sequences may play an important role in expression of individual crystal proteins in B. thuringiensis without doubt.

키워드

참고문헌

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