International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Genetic Homogeneity of the Korean Native Bumble Bee, Bombus ardens (Hymenoptera: Apidae), Detected by Mitochondrial COI Gene Sequences

  • Yoon, Hyung-Joo (Department of Sericulture and Entomology, The National Institute of Agricultural Science & Technology, Rural Development Administration) ;
  • Kim, Sam-Eun (Department of Sericulture and Entomology, The National Institute of Agricultural Science & Technology, Rural Development Administration) ;
  • Lee, Myeong-Lyeol (Department of Sericulture and Entomology, The National Institute of Agricultural Science & Technology, Rural Development Administration) ;
  • Kim, Iksoo (Department of Sericulture and Entomology, The National Institute of Agricultural Science & Technology, Rural Development Administration) ;
  • Bae, Jin-Sik (College of Natural Resources and Life Science, Dong-A University) ;
  • Sohn, Hung-Dae (College of Natural Resources and Life Science, Dong-A University) ;
  • Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University)
  • Published : 2003.03.01
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Abstract

We investigated the sequence divergence of the geographic samples of the queen bumble bee (Bombus ardens) in Korea. A portion of mitochondrial COI gene sequences (423 bp) was analyzed for 44 individuals collected from seven localities. Sequence analysis resulted in four COI haplotypes with the maximum nucleotide divergence of only 0.5% (two bp). One haplotype (BA1) was dominant in all localities surveyed (86.4%). The finding of low sequence divergence and dominance of one haplotype appear to reflect, although limited, the life history of the B. ardens queens subjected to active dispersal and seasonal fluctuation in queen number.

Keywords

References

  1. Allen, T., S. Cameron, R. McGinley and B. Heinrich (1978) The role of workers and new queens in the ergonomics of a bumblebee colony (Hymenoptera: Apoidea). J. Kans. Entomol. Soc. 51, 329-342
  2. Avise, J. C. (1994) Molecular markers, natural history and evolution. Champman & Hall, New York, USA, pp. 204-210
  3. Bae, J. S., I. Kim, S. R. Kim, B. R. Jin and H. D. Sohn (2001) Mitochondhal DNA sequence variation of the mushroom pest flies, Lycoriella mali (Diptera: Sciaridae) and Coboldia fuscipes (Diptera: Scatopsidae), in Korea. AppI. Entomol. ZooI 36,451-457 https://doi.org/10.1303/aez.2001.451
  4. Brower, A. V. Z. (1994) Phylogeny of Heliconius butterflies inferred from mitochondrial DNA sequences (Lepidoptera: Nymphalidae). MoI Phyl. Evol. 3, 159-174 https://doi.org/10.1006/mpev.1994.1018
  5. Brown, W. M., M. George and A. C. Wilson (1982) Mitochon drial DNA sequence ofprimates: tempo and mode ofevolu tion. J. Mol. Evol. 18, 225-239 https://doi.org/10.1007/BF01734101
  6. Hannan, M. A., Y. Maeta and K. Hoshikawa (1998) Feeding behavior and food consumption in Bombus ignitus under artificial condition (Hymenoptera: Apidae). EntomoI. Sci. 1, 27-32
  7. Harrison, R. G. (1989) Animal mitochondrial DNA as a genetic marker in population and evolutionary biology. Trends Ecol. EvoI. 4, 6-11 https://doi.org/10.1016/0169-5347(89)90006-2
  8. Heinrich, B. (1979) Bumblebee economics. Harvard University Press, Cambhdge, Massachusetts, and London, England, pp. 23-54
  9. Iwasaki, M. (1995) Introduction of commercial bumblebees into Japan. Honeybee Sci. 16, 17-21
  10. Kim, J. G., I. Kim, J. S. Bae, B. R. Jin, K. Y. Kim, S. E. Kim, Y.C. Choi, J. Y. Choi, K. Y. Lee, H. D. Sohn and S. K. Noh (2001) Genetic subdivision of the firefly, Luciola tateralis (Coleoptera: Lampyridae), in Korea detected by mitochon-drial COI gene sequences. Korean J. Genetics 23, 203-219
  11. Kim, I., J. S. Bae, K. H. Choi, B. R. Jin, K. R. Lee and H. D. Sohn (2000a) Haplotype diversity and gene flow of the diamondback moth, PlutelIa xylostella (L.) (Lepidoptera:Yponomeutidae), in Korea. Korean J. Appㅣ. Entomol. 39,43-52
  12. Kim, I., J. S. Bae, K. H. Choi, S. R. Kim, B. R. Jin, K. R. Lee and H. D. Sohn (2000b) Mitochondrial DNA polymorphism, gene flow, and population genetic structure of the diamondback moths, PIuteIIa xylostella, (Lepidoptera: Yponomeutidae), in Southern Korea. Korean J. Entomol. 30, 21-3
  13. Kim, I., J. S. Bae, H. D. Sohn, P. D. Kang, K. S. Ryu, H. D Sohn, W. B. Jeong and B. R. Jin (2000c) Genetic homogeneity in the domestic silkworm, Bombyx mori, and phylogenetic relationship between B. mori and the wild silkworm, B. mandarina using mitochondrial COI gene sequences. Int. J.Indust. Entomol. 1, 9-17
  14. Kim, I., S. C. Lee, J. S. Bae, B. R. Jin, S. E. Kim, J. K. Kirn, H. J. Yoon, S. R. Yang, S. H. Lim and H. D. Sohn (2000d) Genetic divergence and phylogenetic relationships among the Korean fireflies, Hotaria papariensis, Luciola lateralis, and Pyrocoelia rufa (Coleoptera: Lampyridae) using mitochondrial DNA sequences. Korean J. Appl. EntomoI. 39, 211-226
  15. Kim, S. E., H. J. Yoon, H. S. Lee, S. B. Lee and I. G. Park(2002) Occurring season of overwintered bumblebee queens in Korea and their visiting flowers. Korean J. Appl. Entomol 41, 191-197
  16. Kruger, E. (1917) Zur Systematik der mitteleuropaischen Hummeln (Hym.). Entomol. Mitt. 6, 55-66
  17. Labougle, J. M. (1990) Bombus of Mexico and Central America (Hymenoptera: Apidae). Univ. Kansas Sci. BuIl. 54, 35-73
  18. Laverty, T. M. and L. D. Harder (1988) The bumble bee of eastem Canada. Can. EntomoI 120, 965-987 https://doi.org/10.4039/Ent120965-11
  19. Lee, S. C., I. Kim, J. S. Bae, B. R. Jin, S. E. Kim, J. K. Kim, H. J. Yoon, S. R. Yang, S. H. Lim and H. D. Sohn (2000) Mitochondrial DNA sequence variation of the firefly, Pyrocoetia rufa (Coleoptera: Lampyridae). Korean J. Appl. Entomol. 39, 181-19
  20. Lee, S. H. and L. Dumouhel (1999) Taxonomic review of Genus Bombus (Hymenoptera: Apidae) from Korea. Ins. Koreana 16, 77-101
  21. Mamyama, T. and M. Kimura (1980) Genetic variability and effective population size when local extinction and recolonization of subpopulations are frequent. Proc. Natl. Acad. Sci. USA 77, 6710-6714 https://doi.org/10.1073/pnas.77.11.6710
  22. Mitsuhata, M. (2000) Pollination of crops with bumblebee colonies in Japan. Honeybee Sci. 21, 17-25
  23. Mun, J. H., Y. H. Song, K. L. Heong and G. K. Roderick (1999) Genetic vahation among Asian populations of rice planthoppers, Nilaparvata lugens and SogateIIa furcifera(Hemiptera: Delphacidae): mitochondrial DNA sequences. Bull. Entomol. Res. 89, 245-253
  24. Sakagami, S. F. (1976) Specific differences in the bionomic characters of bumblebees. A comparative review. J. Fac. Sci. Hokkaido Univ. 20, 390-447
  25. Simon, C., F. Frati, A. Beckenbach, B. Crespi, H. Liu and P. Flook (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a composition of conserved polymerase chain reaction primers. Ann. Entomol.Soc.Aw.87,651-701 https://doi.org/10.1093/aesa/87.6.651
  26. Sperling, F. A. H. and D. A. Hickey (1994) Mitochondrial DNA sequence variation in the spruce budworm species complex (Choristoneura: Lepidoptera). Mol Biol Evol. 11, 656-665
  27. Starr, C. K. (1992) The bumble bees (Hymenoptera: Apidae) of Taiwan. BulI. NatI. Mus. Nat. Sci. 3, 139-157
  28. Williams, P. H. (1994) Phylogenetic-relationships among bumble bees (Bombus Latr.): A reappraisal of morphological evidence. Syst. EntomoI. 19, 327-344 https://doi.org/10.1111/j.1365-3113.1994.tb00594.x
  29. Wilson, A. C., R. L. Cann, S. M. Carr, M. George, U. B. Gyllenstein, K. M. Helm-Bychowski, R. G. Highchi, S. R. Palumbi, E. M. Prager, R. D. Sage and M. Stoneking (1985) Mitochondhal DNA and two perspectives on evolutionary genetics. Biol J. Linn. Soc. 26, 375-400 https://doi.org/10.1111/j.1095-8312.1985.tb02048.x
  30. Yoon, H. J., J. S. Bae, I. Kim, B. R. Jin, Y. I. Mah, J. Y. Moon and H. D. Sohn (2001) A phylogenetic study in some longhomed beetles (Coleoptera: Cerambycidae) using mitochondrial COI gene and 16S rRNA sequences. Int. J. Indust. Entomol. 2, 37-53
  31. Yoon, H. J., S. E. Kim and Y. S. Kim (2002) Temperature and humidity favorable for colony development of the indoorreared bumblebee, Bombus ignitus. AppI. EntomoI. Zool 37, 419-423 https://doi.org/10.1303/aez.2002.419
  32. Yoon, H. J., Y. I. Mah and M. Y. Lee (1998) Some ecological characteristics of Bombus ardens Smith in Korea. Korean J. ApicuIt. 13, 35-42
  33. Yoon, H. J., Y. I. Mah and M. Bilinski (1999a) ESect ofhoneybee workers (Apis miIlifera L.) as helpers on the oviposition, colony foundation and development of the Korean native bumblebee, Bombus ardens Smith. Korean J. Apicult. 14, 105-108
  34. Yoon, H. L, Y. I. Mah, M. Y. Lee, I. G. Park and M. Bilinski(1999b) Ecological characteristics of Bombus ignitus Smith in Korea. Korean J. Appl. Entomol. 38, 101-107