Pulsed-Field Gel Electrophoresis and Mutation Typing of gyrA Gene of Quinolone-Resistant Salmonella enterica Serovar Paratyphi A Isolated from Outbreak and Sporadic Cases,1998-2002, Korea

  • KIM SHUKHO (Laboratory of Enteric Infections, Department of Microbiology, National Institute of Health) ;
  • OK YOUNG LIM (Laboratory of Enteric Infections, Department of Microbiology, National Institute of Health) ;
  • SEONG HAN KIM (Laboratory of Enteric Infections, Department of Microbiology, National Institute of Health) ;
  • JUN YOUNG KIM (Laboratory of Enteric Infections, Department of Microbiology, National Institute of Health) ;
  • YEON HO KANG (Laboratory of Enteric Infections, Department of Microbiology, National Institute of Health) ;
  • BOK KWON LEE (Laboratory of Enteric Infections, Department of Microbiology, National Institute of Health)
  • 발행 : 2003.02.01

초록

In early 2002, over 200 people in the city of Pusan. Korea suffered from paratyphoid fever resulting from Salmonella Paratyphi A Infection. Antimicrobial susceptibility tests and Xbal pulsed-field gel electrophoresis (PFCE) were conducted to 54 Salmonella Paratyphi A isolated from humans during the period of 1998 to 2002. Most of the isolates ($83\%$) were only nalidixic acid-resistant and $78\%$ were X 1 PFGE patterns. Also, we measured the MIC of ciprofloxacin and screened gyrA mutation(5) using allele- specific PCR and restriction fragment length polymorphism (AS-PCR-RFLP). The representative 5 isolates in 2002 and 1 isolate in 2000 were $1{\mu}g/ml$ of MIC and had mutation at the 83rd codon in gyrA. These data suggest that the outbreak in the early 2002 might have been due to dissemination of the strain present In 2000. Also, decreased susceptibility to ciprofloxacin was partly due to the mutation at the 83rd codon in gyrA.

키워드

참고문헌

  1. Lancet v.359 Experts call for surveillance of drugresistant typhoid at a global level Ahmad, K.
  2. J. Antimicrob. Chemother. v.25 The Successful treatment of multiresistant nonenteric salmonellosis with seven day oral ciprofloxacin Barnass, S.;J. Franklin;S. Tabaqchali https://doi.org/10.1093/jac/25.2.299
  3. Trop. Gastroenterol v.21 Role of molecular typing in an outbreak of Salmonella paratyphi A Chandel, D. S.;N. Nisar;K. L. Thong;T. Pang;R. Chaundhry
  4. Emerg. Infect. Dis. v.6 Drug-resistant Salmonella enterica serotype Paratyphi A in India Chandel, D. S.;R. Chaundhry;B. Dhawan;A. Pandey;A. B. Dey https://doi.org/10.3201/eid0604.000420
  5. Rev. Infect. Dis. v.13 Quinolones for the treatment of infections due to salmonella Cherubin, C. E.;R. H. Eng. https://doi.org/10.1093/clinids/13.2.343
  6. J. Clin. Microbiol. v.35 Rapid pulesed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day Gautom, R. K.
  7. Antimicrob. Agents Chemother. v.43 Comparative studies of mutations in animal isolates and experimental in vitro- and in vivo-selected mutants of Salmonella spp. suggest a counterselection of highly fluoroquinolone-resistant strains in the field Giraud, E.;A. Brisabois;J. L. Martel;E. Chaslus-Dancla
  8. J. Appl. Microbiol v.92 Genetic diversity of salmonella enterica serovar Paratyphi A from different geographical regions in Asia Goh, Y. L.;S. D. Puthucheary;R. Chaundhry;Z. A. Bhutta;M. Lesmana;B. A. Oyofo;N. H. Punjabi;A. Ahmed;K. L. Thong https://doi.org/10.1046/j.1365-2672.2002.01655.x
  9. Antimicrob. Agents Chemother. v.40 Mutations in gyrA gene of quinolone-resistant Salmonella serotypes isolated from humans and animals Griggs, D. J.;K. Gensberg;L. J. Piddock
  10. Harrison's Principles of Internal Medicine Guerrant, R. L.
  11. Emerg. Infect. Dis. v.3 Emerging quinolone-resistants Salmonella in the United States Herikstad, H.;P. Hayes;M. Mokhtar;M. L. Fracaro;E. J. Threlfall;F. J. Angulo https://doi.org/10.3201/eid0303.970316
  12. Antimicrob. Agents Chemother. v.45 Antibiotic susceptibilities of Salmonella enterica serovar Typhi and S. enterica serover Paratyphi A isolated from patients in Japan Hirose, K.;K. Tamura;H. Sagara;H. Watanabe https://doi.org/10.1128/AAC.45.3.956-958.2001
  13. Encyclopedia of Immunology v.III Hormaeche, C. E.
  14. Emerg. Infect. Dis. v.3 Paratyphoid fever due to Salmonella enterica serotype Paratyphi A kapil, A.;S. Sood;V. P. Reddaiah;B. Das;P. Seth
  15. J. Microbiol. Biotechnol. v.12 Epidemiological typing and characterization of dfr genes of shigella sonnei isolates in Korea during the last two decades Kim, K. S.;J. Y. Oh;Y. W. Jeong;J. W. Cho;J. C. Park;D. T. Cho;J. C. Lee
  16. Lancet v.351 Epidemic ciprofloxacin-resistant Salmonella typhi in Tajikistan Merdoch, D. A.;N. Banatvaia;A. Bone;B. I. Shoismatulloev;L. R. Ward;E. J. Threlfall;N. A. Banatvala https://doi.org/10.1016/S0140-6736(05)78338-0
  17. National Committee for Clinical Laboratory Standards(2nd ed.) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically National Committee for Clinical Laboratory Standards
  18. approved standards, 7th ed. NCCLS document M2-A7 Performance standards for antimicrobial disk susceptibility test National Committee for Clinical Laboratory Standards
  19. J. Microbiol. Biotechnol. v.5 Restriction fragment fingerprint of an alkaliphilic Micrococcus sp. Y-1 genome by pulsed-field gel electrophoresis Park, J. H.;C. H. Kim
  20. Lancet v.351 Quinolone-resistant Salmonella typhi in Vietnam Parry, C.;J. Wain;N. T. Chinh;H. Vinh;J. J. Farrar
  21. Antimicrob. Agents Chemother v.39 Salmonella typhimurium gyrA mutations associated with fluoroquinolone resistance Reyna, F.;M. Huesca;V. Gonzalez;L. Y. Fuchs https://doi.org/10.1128/AAC.39.7.1621
  22. J. Microbiol. Biotechnol. v.11 Identification of Streptomyces sp. AMLK-335 producing antibiotic substance inhibitory to vancomycin-resistant enterrococci Rhee, K. H.;K. H. Choi;C. J. Kim;C. H. Kim
  23. Rev. Infect. Dis. v.11 no.5 Use of quinolones for the treatment of patients with bacteremia Shah, P. M. https://doi.org/10.1093/clinids/11.Supplement_5.S1156
  24. Ann. Acad. Med. Singapore v.26 A nationwide outbreak of coconut-associated paratyphoid A fever in Singapore Teoh, Y. L.;K. T. Goh;K. S. Neo;M. Yeo
  25. Emerg. Infect. Dis. v.4 Molecular analysis of Salmonella paratyphi A from an outbreak in New Delhi, India Thong, K. L.;S. Nair;R. Chaundhry;P. Seth;A. Kapil;D. Kumar;H. Kapoor;S. Puthucheary;T. Pang https://doi.org/10.3201/eid0403.980348
  26. Lancet v.353 Ciprofloxacin-resistant Salmonella typhi and treatment failure Threlfall, E. J.;L. R. Ward;J. A. Skinner;H. R. Smith;S. Lacey https://doi.org/10.1016/S0140-6736(99)01001-6
  27. Clin. Infect. Dis. v.25 Quinolone-resistant Salmonella typhi in Viet Nam: molecular basis of resistance and clinical reponse to treatment Wain, J.;N. T. Hoa;N. T. Chinh;H. Vinh;M. J. Everett;T. S. Diep;N. P. Day;T. Solomon;N. J. White;L. J. Piddock;C. M. Parry https://doi.org/10.1086/516128