Biotechnology and Bioprocess Engineering:BBE

  • 제8권1호
  • /
  • Pages.47-53
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  • 2003
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Chromatographic Separation of Maltopentaose from Maltooligosaccharides

  • Lee, Jae-Wook (Department of Chemical Engineering, Seonam University) ;
  • Kwon, Tae-Ouk (Department of Chemical Engineering, Sunchon National University) ;
  • Moon, Il-Shik (Department of Chemical Engineering, Sunchon National University)
  • 발행 : 2003.01.01
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초록

An experimental study on the chromatographic separation of maltopentaose from a mixture, including glucose, maltose, maltotriose, and maltopentaose, was carried out in a nonionic polymeric sorbent column while varying the operating conditions, such as the solution pH, buffer contents, and isopropyl alcohol (1PA) concentration. Unlike the pH and buffer contents, the IPA concentration had a Significant impact on the single component chromatograms for maltopentaose. The retention times of the maltooligosaccharides with the nonionic polymeric sorbent Sp207 were in the following order: glucose < maltose < maltotriose < maltopentaose. From the experimental binary, ternary, and quaternary chromatograms, gradient chromatographic separation with a changing IPA concentration as a function of time was required to obtain high-purity maltopentaose and reduce the elution time.

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