KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Optimum Conditions for the Simultaneous Saccharification and fermentation of Paper Sludge and Fermentation of paper Sludge to Produce lactic acid and viable Lactobacillus cells

제지 슬러지의 동시당화발효에서 젖산과 유산균 생산을 위한 최적 배양 조건

  • 정다연 (인하대학교 생물공학과 초정밀생물분리기술연구센터) ;
  • 이상목 (인하대학교 생물공학과 초정밀생물분리기술연구센터) ;
  • 구윤모 (인하대학교 생물공학과 초정밀생물분리기술연구센터) ;
  • 소재성 (인하대학교 생물공학과 초정밀생물분리기술연구센터)
  • Published : 2003.02.01
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Abstract

In this study of the simultaneous saccharification and fermentation (SSF) of paper sludge, fed-batch cultivation of Lactobacillus paracasei subsp. paracasei KLB58 was attempted to produce viable KLB58 cells and lactic acid. Optimal culture conditions, including the temperature and concentration of the supplemented enzyme, were examined in terms of lactic acid production and viable cell count. When the effects of culture temperature and $\beta$-glucosidase concentration were examined in fed-batch SSF, the highest viable cell counts and lactic acid production (i.e. 5$\times$$10^9$ CFU/ml and 45 g/L, respectively) were obtained at 37$^{\circ}C$ and 2 unit/ml of $\beta$-glucosidase.

본 연구에서는 제지 슬러지를 이용한 SSF 공정에 L..paracasei KLB58을 적용하여 젖산과 더불어 생균제용 균체를 경제적으로 대량 생산하고자 하였다. KLB58의 배양온도와 섬유소 가수분해 효소인 $\beta$-glucosidase의 농도를 조절하여 최적의 생산 조건을 확인해 본 결과, 37$^{\circ}C$ 에서 2 unit/ml의 $\beta$-glucosidase를 첨가하여 배양하였을 때 최대의 젖산과 균체를 생산하였다. 또한 $\beta$-glucosidase를 포함하지 않아도 상대적으로 많은 양의 젖산과 균체를 생산하였으므로, 이에 대한향후 연구가 기대된다.

Keywords

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