미생물학회지 (Korean Journal of Microbiology)

  • 제39권1호
  • /
  • Pages.62-65
  • /
  • 2003
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  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

피막 다당류 6B-단백질 복합체를 이용한 폐렴구균에 대한 Multibead Assay의 안정성 향상

Improvement of the Stability of the Multibead Assay for Pneumococci by the Use of 6B-protein Complex

  • 김지혜 (전북대학교 기초과학연구소) ;
  • 임낙룡 (전북대학교 사범대학 과학교육학부) ;
  • 박문국 (전북대학교 자연과학대학 생물과학부)
  • 발행 : 2003.03.01
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초록

폐렴구균의 혈청형 판별법인 multibead assay에서 사용하는 미세구슬 표면에 코팅한 다당류의 안정성을 높이기 위한 방안을 연구하였다. 폐렴구균 피막 다당류 6B에 bovine serum albumin (BSA)을 결합시킨 다당류-단백질 복합체로 코팅했을 경우와 기존의 방법인 다당류만으로 코팅했을 경우의 코팅 효율과 미세구슬표면에서의 6B 안정성을 비교하였다. 다당류 6B-BSA 복합체를 사용했을 경우에 코팅 효율은 약 200 배 증가하였으며, 미세구슬 표면에서의 6B 안정성도 증가하여, 한번 코팅 후 미세구슬을 사용할 수 있는 기간이 3 일에서 30 일 이상으로 연장될 수 있음을 확인하였다.

A procedure to increase the stability of 6B capsular polysaccharide on microbead surface in the mutibead assay, a serotyping method for Streptococcus pneumoniae, was studied. Pneumococcal capsular polysaccharide 6B was conjugated to bvine serum albumin (BSA), and the coating efficiency and the stability of the 6B-BSA complex was measured. The 6B-BSA complex showed about 200-fold higher coating efficiency to polystyrene surface than 6B polysaccharide. And the stability of the 6B- BSA to be used in the multibead assay for 30 days after coating.

키워드

참고문헌

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