Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Biologically Active Fractions from Onion on Physiological Activity and Lipid Metabolism

양파로부터 분리한 생리활성분획이 생리활성 및 지질대사에 미치는 영향

  • Woo, Hi-Seob (School of Food Science, Dongju College) ;
  • Aan, Bong-Jeun (Faculty of Life Resources and Engineering, Kyungsan University) ;
  • Bae, Jong-Ho (Dept. of Confectionary Decoration, Daegu Future College) ;
  • Kim, Sung (Dept. of Food Science and Technology, Yeungnam University) ;
  • Choi, Hee-Jin (Dept. of Food Science and Technology, Yeungnam University) ;
  • Han, Ho-Suk (Dept. of Food Science and Technology, Yeungnam University) ;
  • Choi, Cheong (Dept. of Food Science and Technology, Yeungnam University)
  • 우희섭 (동주대학 식품과학계열) ;
  • 안봉전 (경산대학교 생명자원공학부) ;
  • 배종호 (대구미래대학 제과데코레이션과) ;
  • 김성 (영남대학교 생물산업공학부) ;
  • 최희진 (영남대학교 생물산업공학부) ;
  • 한호석 (영남대학교 생물산업공학부) ;
  • 최청 (영남대학교 생물산업공학부)
  • Published : 2003.02.01
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Abstract

From the 80% ethanol extraction of onion flesh, fraction I,II and III were separated by Sephadex LH-20 column chromatography. Fraction II and III had a high inhibitory effect on ACE and xanthine oxidase. The ACE was about 80% at 50 ppm of fraction IIand III. The xanthine oxidase was about 82.5% at 100 ppm of fraction In. This research was studied to investigate the effects of the fraction In isolated from onion on the reduction of fat accumulation in rats fed high fat diet for 2 months. The metabolism was examined by analyzing the fat in serum and liver. It was shown that cholesterol in serum was remarkably reduced in onion fraction In as compared with those of control.

양파를 80% ethanol로 추출하여 Sephadek LH-20 column chromatography로 fraction I, II 및 III의 생리활성물질을 분리하여 angiotensin converting enzyme(ACE), xanthine oxidase, tyrosinase의 저해작용 효과와 지질대사를 검토하였다 Fraction I과 II의 ACE 저해작용은 50 ppm 농도에서 80% 이상의 효소 저해효과를 나타내었고 fraction III에서는 500 ppm 농도에서 90% 효소저해작용을 보였다. 100 ppm 농도에서 fraction III의 xantine oxidase 효소저해 효과는 82.5%로 나타났으며 tyrosinase의 저해효과는 42.6%이었다. 효소저해작용이 가장 강한 fraction III를 2개월 동안 고지방식이에 첨가하여 혈장 및 간장에서 지질대사에 미치는 영향을 분석한 결과 혈장에서는 총지질, 중성지질이 대조군에 비하여 유의성 있게 감소하였다. 간의 콜레스테롤량도 약 50% 정도 감소하는 결과를 얻었다.

Keywords

References

  1. Kee HJ, Park YK. 2000. Preparation and quality properties of extruded snack using onion pomace and onion. Kor J Food Sci Technol 32: 578-583.
  2. Kwak HJ, Kwon YJ, Jeong PH, Kwon JH, Kim HK. 2000. Physiological activity and antioxidative effect of methanol extract from onion (Allium cepa L.). J Kor Soc Food Sci Nutr 29: 349-345.
  3. Ra KS, Suh HJ, Chung SH, Son JY. 1997. Antioxidant activity of solvent extract from onion skin. Kor J Food Sci Technol 29: 595-600.
  4. Leighton T, Ginther C, Fluss L, Harter WK, Cansado J,Nortrio V. 1992. Molecular characterization of quercetin andquercetrin glycosides in allium vegetables, phenolic compoundsin food and their effects on health II. ACS, Washington,D.C. p 221.
  5. Patall BS, Pike LM. 1995. Distribution of quercetin contentin different rings of various coloured onion (Allium cepaL.) cultivars. J Horticultural Sci 70: 643-650. https://doi.org/10.1080/14620316.1995.11515338
  6. Ha YL, Kim JO, Kim HD, Suh JK, Byon JI, Park SJ, ParkKA. 1996. Analytical and extraction methods of bioactivesubstances for onion processings. RDA J Agric Sci (AgricInst Cooporation) 38: 171-185.
  7. Kim SO, Lee MY. 2001. Effect of ethylacetate fraction of onion on lipid metabolism in high cholesterol fed rats. J Kor Soc Food Sci Nutr 30: 673-678.
  8. Kim KM, Suh HJ, Chung SH, Cho WD, Ma SJ. 1999. Chemical structure of angiotensin converting enzyme inhibitor isolated from onion flesh. Food Sci Biotechnol 8: 329-332.
  9. Ma SJ. 2000. Inhibitory effect of onion seasoning on angiotensin converting enzyme. J Kor Soc Food Sci Nutr 29: 395-400.
  10. Lee CJ, Kim HD, Choung EH, Suh JK, Park CW, Ha JK. 2000. Reduction effect of carcinogen induced mouse epidermal and forestomach carcinogenesis by the extract of onion wastes. J Kor Soc Food Soc Nutr 29: 525-530.
  11. Rho SN, Han JH. 2000. Cyotoxicity of garlic and onion methanol extract on human lung cancer cell lines. J Kor Soc Food Soc Nutr 29: 870-874.
  12. Lee YK, Lee HS. 1990. Effect of onion and ginger on the lipid peroxidation and fatty acid composition of mackerel during frozen storage. J Kor Soc Food Nutr 19: 321-329.
  13. Sheo HJ, Jung DL. 1977. The effect of onion juice on serum lipid levels in rats. J Kor Soc Food Sci Nutr 26: 1164-1172.
  14. Rao SS, Venkatainaman PR. 1946. Investigation on antibiotics,studies on allicin, the antibacterial principles ofAllium sativum L. J Sc Ind (India) 18: 31.
  15. Lee SH, Woo SJ, Koo YJ, Shin HK. 1995. Effets of mugwort,onion and polygalate radix on the intestinal environmentof rats. Kor J Food Sci Technol 27: 598-604.
  16. Sheo HJ, Lim HJ, Jung DL. 1993. Effect of onion juice on toxicity of lead in rat. J Kor Soc Food Nutr 22: 138-143.
  17. Park PS, Lee BR, Lee MY. 1991. Effects of onion diet on carbon tetrachloride toxicity of rats. J Kor Food Nutr 20: 121-125.
  18. Park PS, Lee BR, Lee MY. 1994. Effects of onion juice on ethanol-induced hepatic lipid peroxidation in rats. J Kor Food Nutr 23: 750-756.
  19. Swain T, Hillis WE, Oritega M. 1959. Phenolic constituentsof Ptunus domestica. I. Quantitative analysis of phenolicconstituents. J Sci Food Agric 10: 83-86.
  20. Cushman DW, Ondetti MA. 1980. Inhibitors of angiotensinconverting enzyme for treatment of hypertension. BiochemPharmacology 29: 1871-1877. https://doi.org/10.1016/0006-2952(80)90096-9
  21. Stripe F, Corte ED. 1969. The regulation of rat liver xanthineoxidase. J Biol Chem 244: 2855-3859.
  22. Yagi K. 1987. Lipid peroxides and human disease. ChemPhuys Lipids 45: 337-341. https://doi.org/10.1016/0009-3084(87)90071-5
  23. Frings CS. 1970. A colorimetric method for determinationof total serum lipid based on the sulfophospho-vanillin reaction.Am J Clin Pathol 53: 80-91.
  24. An BJ, Lee JT, Bea MJ. 1998. Isolation of novel polyphenol from oolong tea and its effective prevention of the gout. Kor J Food Sci Technol 30: 970-975.
  25. Kim JK, Cha WS, Park JH, Oh SL, Cho YJ, Chun SS, Choi C. 1997. Inhibition effect against tyrosinase of condensed tannins from Korean green tea. Kor J Food sci Technol 20: 173-177.
  26. Nicolas G, Bazan Jr. 1970. Effects of ischemia and electroconvulsiveshock on free fatty acid and pool in the brain.Biochem Biophy Acta 282: 1128-1140.
  27. Kim SY, Kim HS, Kim SH, Kim HS, Su IS, Chung SY. 1993. Effects of the feeding Plantycodon grandiflorum and Codonopsis lanceolata on the fatty acid composition of serum and liver in rats. J Kor Soc Food Nutr 22: 524-530.

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