Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Detection of Radiation Induced Markers in Oranges Imported from the United States of America

미국산 오렌지의 Radiation Induced Marker 검색

  • Published : 2003.02.01
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Abstract

Radiation induced markers were investigated for the detection of irradiated oranges imported from America. In the DNA comet assay, the non-irradiated and irradiated samples showed the comets with long tails in both seed and flesh. Though this tendency was maintained for 6 weeks, identification of non-irradiated or irradiated samples was impossible. In the thermoluminescence (TL) measurement, the non-irradiated samples revealed a glow curve with low intensity at about 28$0^{\circ}C$, while the irradiated samples showed with higher intensity at around 18$0^{\circ}C$. There were no remarkable changes in detection properties for 6 weeks after irradiation. The TL ratio of area for TL$_1$ glow curve to TL$_2$ was below 0.1 for the non-irradiated samples and 0.5 or more for the irradiated ones during storage. In the electron spin resonance (RSR) measurement, irradiated oranges showed an unspecific central signal in all parts (seed, flesh and peel), so the detection for radiation treatment of oranges was impossible. Based on the results, DNA comet assay and ESR were not useful for the detection, but TL was appropriate to search radiation induced markers of oranges during storage period. The detectable period during storage is confirmed by sensory evaluation.

교역량이 증가되고 있는 수입 오렌지를 대상으로 radiation induced marker검색을 위하여, 방사선 조사시 예상되는 생물학적, 물리적 변화에 기초한 DNA comet assay, 열발광 (TL) 및 전자스핀공명 (ESR) 분석을 실시하였다. DNA comet assay 결과, 오렌지의 씨 및 과육의 cell은 감마선 조사와 무관하게 긴 tail을 가진 comet 모양으로 관찰되었으며, 저온 저장 6주까지도 유사한 경향이 유지되어 marker로의 활용 가능성이 낮았다. TL 측정 에서 비조사 시료는 약 200~30$0^{\circ}C$ 부근에서 매우 낮은 intensity의 glow curve를 나타내었고, 0.5 kGy 이상의 조사 시료에서는 조사 시료 특유의 peak가 18$0^{\circ}C$ 부근에서 나타났으며, 조사 선량의 증가에 따라 intensity 도 점차 증가하였다 TL 측정은 저장 6주까지도 가능하였고, TL ratio(TL$_1$/TL$_2$)의 산출은 검지 결과의 신뢰도를 높여주었다. ESR 측정에서는 시료의 씨, 과육 및 과피 부위에서 방사선 조사 유래의 특이적인 signal이 나타나지 않아 조사 시료와 비조사 시료의 구분이 불가능하였다. 이상의 결과에서 볼때 오렌지는 TL 분석에 의해 방사선 조사 여부의 확인이 가능하며 이 방법은 오렌지의 저온 상품성이 유지되는 저장6주 이후에도 적용이 가능한 것으로 나타났다.

Keywords

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