Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Protective Effect of Yellow-Green Vegetable Juices on DNA Damage in Chinese Hamster Lung Cell Using Comet Assay

Comet Assay를 이용한 케일, 명일엽, 당근, 돌미나리 녹즙의 Chinese Hamster Lung 세포 DNA 손상 보호 효과

  • 전은재 (한남대학교 이과대학 식품영양학과) ;
  • 김정신 (한남대학교 이과대학 식품영양학과) ;
  • 박유경 (한남대학교 이과대학 식품영양학과) ;
  • 김태석 ((주)풀무원 기술연구소 생물공학연구팀) ;
  • 강명희 (한남대학교 이과대학 식품영양학과)
  • Published : 2003.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

The present study was attempted to investigate the antioxidant capacity of popular yellow-green vegetable juices (kale, Angelica keishei, carrot, small water dropwort) and to investigate the effect of vegetable juices on protecting oxidative damage to DNA in cultured Chinese hamster lung (CHL) cells. Antioxidant capacity was analyzed by TRAP assay (Total radical-trapping antioxidant potential). Cellular DNA dmamage was measured by SCGE (single-cell gel electrophoresis, also known as comet assay. Cells incubated in medium with PBS (negative control) or with various concentration of the freeze dried green juices (25, 50, 100, 250 $\mu\textrm{g}$/$m\ell$) resuspended in PBS were treated with $H_2O_2$ (200 ${\mu}{\textrm}{m}$) as an oxidative stimulus for 5 min at 4$^{\circ}C$. The physiological function of each vegetable juice on oxidative DNA damage was analyzed and expressed as tail moment (tail length X percentage migrated DNA in tail) . Kale juice had the highest TRAP value suggesting that kale has the highest antioxidant capacity followed by Angelica keishei, small water dropwort and carrot. Cells treated with $H_2O_2$ had extensive DNA damage compared with cells treated with PBS or pre-treated with vegetable juice extracts. All green juices inhibited $H_2O_2$-induced DNA damage with kale being the most effective juice among the tested juices. These results indicate that green juice supplementation to CHL cells followed by oxidative stimulus inhibited damage to cellular DNA, supporting a protective effect against oxidative damage induced by reactive oxygen species. (Korean J Nutrition 36(1) : 24-31, 2003)

Keywords

References

  1. Food and Hygiene v.6 The effect of green juice Yang HS.
  2. Korean J Food Sci Technol v.31 no.4 Analysis of antioxidant nutrients in green yellow vegetable juice Chung SY;Kim HW;Yoon S.
  3. Korean J Food Sci Technol v.24 The nutritional component of Angelica keiskei koidz Kim OK;Kung SS;Park WB;Lee MW;Han SS.
  4. Korean J Food Sci Technol v.27 Changes of contents of β-carotene and vitamin C and antioxidative activities of juice of Angelica keiskei Koidz stored at different conditions Park WB;Kim DS.
  5. Korean J Food Sci Technol v.26 no.5 Antimutagenic effect and active compound analysis of kale juice in salmonella system Lee SM;Park KY;Rhee SH.
  6. Korean J Food Safety v.8 Antimutagenicity of small water dropwort juice on the microbial mutagenicity induced by 2-aminofluorene Han KS;Cheong EH;Ham SS;Shim TH;Lee TS;Lee HK.
  7. Planta Med v.57 Antitumor-promotion by principles obtained from Angelica keiskei Okuyama T;Takada M. https://doi.org/10.1055/s-2006-960082
  8. J Korean Soc Food Sci Nutr v.19 no.5 Effects of water soluble extract of ganoderma lucidum, kale juice and sodium dextrothyroxine on hormone and lipid metabolism in hyperchoesterolemic rats 1. concentration of tri-iodothyronine, thyroxine, blood sugar and lipid composition in serum Chung SY;Kim SH;Kim HS;Kang JS;Cheong HS;Kim GJ;Kim HJ.
  9. Korean J Food Sci Technol v.27 Inhibitory effect of Angelica keiskei Koidz green juice on the liver damage in CC14-treated rats Jung HK;Park PS;Huh NC;Kim SO;Kim KS;Lee MY
  10. J Korean Soc Food Sci Nutr v.26 no.2 Effects of Angelica kieskei on Lipid Metabolism in Rats Park JH;Park SK;Cho YS;Chun SS;Choi SH;Park JC.
  11. Mutat Res v.202 Mechanism of inhibition of free-radical processes in mutagenesis and carcinogenesis Simic MG. https://doi.org/10.1016/0027-5107(88)90199-6
  12. Mutat Res v.459 Effects of epigallocatechin gallate and quercetin on oxidative damage to cellular DNA Melissa KJ;George L. https://doi.org/10.1016/S0921-8777(99)00074-9
  13. Mutat Res v.409 The nature and origin of DNA single-strand breaks determined with the comet assay Eva H;Darina S;Lucia H;Tapan KM;Alena G;Andrew RC. https://doi.org/10.1016/S0921-8777(98)00053-6
  14. Mutat Res v.294 Induction and rejoining of radiation-induced DNA single-strand breaks: "tail moment" as function of position in the cell cycle Olive PL;Banath JP. https://doi.org/10.1016/0921-8777(93)90010-E
  15. Radiat Res v.142 Radiation-induced DNA double-strand breaks produced in histone-depleted tumor cell nuclie measured using the neutral comet assay Olive PL;Banath JP. https://doi.org/10.2307/3579022
  16. Korean J Food Sci Tech v.31 Detection of irradiated grains using the DNA 'Comet Assay' Kim CK;Yang JS;Lee HJ.
  17. Mutat Res v.339 The comet assay: a comprehensive review Fairbairn DW;Olive PL;O'Neill KL. https://doi.org/10.1016/0165-1110(94)00013-3
  18. Mutat Res v.288 A European review Mckevery-Martin VJ;Green MHL;Schmezer P;Pool-Zobel BL;De Meo MP;Collins A. https://doi.org/10.1016/0027-5107(93)90207-V
  19. Mutat Res v.393 Quercetin and myricetin protect against hydrogen peroxide induced DNA deamge (strand breaks and oxidised pyrimidives) in human lymphocytes Collins AR;Duthie GG;Dobson VL https://doi.org/10.1016/S1383-5718(97)00107-1
  20. Mutat Res v.390 The effect of dietary flavonoids on DNA damage (strand breaks and oxidised pyrimidines) and growth in human cells Duthie SJ;Johnson W;Dobson VL https://doi.org/10.1016/S0165-1218(97)00010-4
  21. Am J Clin Nutr v.67 Effects of flavonoids and vitamin C on oxidative DNA damage to human lymphocytes Mostafa N;Wilson JA;Michael EJL
  22. Mutat Res v.336 The kinetics of repair of oxidative DNA damage (stand breaks and oxidised pyrimidines) in human cells Collins AR;Ma AG;Duthie SJ. https://doi.org/10.1016/0921-8777(94)00043-6
  23. Mutat Res v.402 The use of single cell gel electrophoresis and flow cytometry to identify antimutagens from commercial soybean by-producs Michael JP;Elizabeth DW;Lynn K;Karen R;Lori CA;Rayburn AL https://doi.org/10.1016/S0027-5107(97)00299-6
  24. Mutat Res v.485 Disparate effects of similar phenolic phytochemicals as inhibitors of oxidative damage to cellular DNA Melissa RK;Jing X;Karen EA;George L. https://doi.org/10.1016/S0921-8777(01)00066-0
  25. Food Chemical Toxicol v.39 Effects of Brussel sprouts extracts on hydrogen peroxide-induced DNA strand breaks in human lymphocytes Zhu CY;Loft S. https://doi.org/10.1016/S0278-6915(01)00061-8
  26. Cancer Epidemiol Biomarkers Prev v.5 Epidemiological studies on brassica vegetables and cancer risk Verhoeven DTH;Goldbohm RA;van Poppel G;Verhagen H;van den Brandt PA
  27. J Korean Soc Food Sci Nutr v.29 no.2 The antigenotoxic effects of Korean native fermented food, baechu kimchi using comet assay Ji ST;Park JH;Hyun CK;Shin HK.
  28. Methods in Enzymol v.234 Total antioxidant status in plasma and body fluids Rice-Evans C;Miller N. https://doi.org/10.1016/0076-6879(94)34095-1
  29. J Agric Food Chem v.33 Distribution of quercetin and kaempferol in lettuce, Kale, chive, garlic chive, leek, horse radish, red radish and red cabbage tissues Bilyk A;Sapera GM. https://doi.org/10.1021/jf00062a017
  30. J Agric Food Chem v.40 Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly comsumed in the Netherland Hertog MGL;Hollman PCH;Katan MB. https://doi.org/10.1021/jf00024a011
  31. J Korean Soc Food Sci Nutr v.28 no.2 Nitrite scavenging effect of methanol fraction obtained from green yellow vegetable juices Chung SY;Kim NK;Yoon S.
  32. J Life Sci v.5 Antimutagenic effect of insoluble dietary fibers from some green yellow vegetables and soybean by binding the carcinogens Lee SM;Lee SH;Park KY;Rhew TH;Kim BK;Chung HY.
  33. Korean J Genetics v.17 no.3 Inhibitory effect of the fresh juice of kale on the genotoxicity of aflatoxin B1 Choi YH;Park KY;Lee SM;Yoo MA;Lee WH.
  34. J Korean Soc Food Sci Nutr v.20 no.1 Effects of water soluble extract of ganoderma lucidum, kale juice and sodium dextrothyroxine on hormone and lipid metabolism in hyperchoesterolemic rats 2. lipid compositions of liver, brain and testis Chung SY;Kim SH;Kim HS;Kang JS;Cheong HS;Kim GJ;Kim HJ.
  35. Korean J Food Sci Technol v.24 The nutritional components of Aerial Whole Plant and juice of Angelica keiskei Koids Kim OK;Kung SS;Park WB;Lee MW;Ham SS.
  36. AOAC v.68 Analyticial methods for determining ascorbic acid in biological samples, food produts and pharmaceuticals Pachla LA;Reyhods DL;Kissing PT.
  37. Korean J of Food Safety Hygene v.8 no.4 Antimutagenicity of small water dropwort juice on the microbial mutagenicity induced by 2-aminofluorene Han KS;Cheong EH;Ham SS;Shim TH;Lee TS;Lee HK.
  38. J Bacteriolo Virol v.28 no.5 Effects of small water dropwort extract on cellular immune response of mice Kim GH;Jang MW;Park GY;Lee SH;Ryu TH;Sunwoo YK.
  39. J Nutr v.119 Review of epidemiology that carotenoids reduce the risk of cancer Ziegler RG.
  40. Cancer v.54 Clinical trials for the evaluation of cancer therapy Fish B. https://doi.org/10.1002/1097-0142(19841201)54:2+<2609::AID-CNCR2820541404>3.0.CO;2-P
  41. Toxicol letters v.112-113 Chemoprevention of cancer: lessons to be learned from beta-carotene trials Vainio H. https://doi.org/10.1016/S0378-4274(99)00208-8
  42. N Eng J Med v.330 The alpha-tocopherol and beta-carotene cancer prevention study group Owens WC;Cross KW. https://doi.org/10.1056/NEJM199404143301501
  43. Nutrition Res v.16 no.4 Serum beta-carotene response to supplementation with raw carrots, carrot juice or purified beta-carotene in healthy non-smoking women Torronen R;Lehmusaho M;Hakkinen S;Hanninen O;Mykkanen H. https://doi.org/10.1016/0271-5317(96)00035-8
  44. Mutat Res v.485 Effect of phytoestrogen and antioxidant supplementation on oxidative DNA damage assessed using the comet assay Sierens J;Hartley JA;Campbell MJ;Leathem AJC;Woodside JV. https://doi.org/10.1016/S0921-8777(00)00069-0
  45. Am J Clin Nutr v.67 Effects of flavonoids and vitamin C on oxidative DNA damage to human lymphocytes Noroozi M;Angerson WJ;Michael EJL.