Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Activities of Enzymes Involved in Fatty Acid Metabolism in the Colon Epithelium of Piglets Fed with Different Fiber Contents Diets

  • Zhu, Y.H. (Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences) ;
  • Lundh, T. (Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences) ;
  • Wang, J.F. (Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences)
  • Received : 2002.02.03
  • Accepted : 2003.04.22
  • Published : 2003.10.01
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Abstract

The present study was conducted to evaluate the influence of dietary fiber on the activities of malic enzyme and citrate lyase involved in fatty acid metabolism in the colon epithelium of pigs. Thirty-six weaned 5 weeks old crossbred (Yorkshire${\times}$Swedish Landrace) piglets originating from twelve litters were randomly assigned to either a low fiber diet containing 10% non-starch polysaccharides (NSP), a control diet containing 14.7% NSP or a high fiber diet containing 20% NSP. The activity of malic enzyme in the colonic epithelium of pigs significantly (p<0.05) increased with age during the suckling-weaning transition. There was a tendency (p<0.10) of decreased malic enzyme activity in the colonic epithelium of pigs fed on the high fiber diet. At week 6, a lowered (p<0.01) activity of malic enzyme in pigs fed on the low fiber diet compared with that in pigs fed on the high fiber and the control diets. Nevertheless, there were no significant differences in the activity of citrate lyase observed either between pigs with different ages or between pigs fed with various diets. The current data suggest that piglets during the suckling-weaning transition have a limited capacity to synthesize fatty acids from carbohydrate derivatives in the coloncytes. In addition, lipogenesis in coloncytes was enhanced with age during the suckling-weaning transition. A tendency (p<0.10) to an increased capacity to utilize acetyl-CoA in coloncytes of pigs has been observed for the high fiber diet. Moreover, the present work indicated that dietary fiber resulted in a lowered rate of lipogenesis and a reduced activity of malic enzyme.

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References

  1. Arbeeny, C. A., D. S. Meysers, K. E. Bergquist and R. E. Gregg. 1992. Inhibition of fatty acid synthesis decreases very-low density lipoprotein secretion in the hamster. J. Lipid Res. 33:843-851.
  2. Association of Official Analytical Chemists. 1990. Official Methods of Analysis. 15th edition. Association of Official Analytical Chemists, Washington DC, USA.
  3. Bach Knudsen, K. E. 1997. Carbohydrate and lignin contents of plant material used in animal feeding. Anim. Feed Sci. Tech. 67:319-338. https://doi.org/10.1016/S0377-8401(97)00009-6
  4. Bach Knudsen, K. E. and I. Hansen. 1991. Gastrointestinal implications in pigs of wheat and oat fractions, I. Digestibility and bulking properties of polysaccharides and other constituents. Br. J. Nutr. 65:217-232.
  5. Bee, G. 2000. Dietary conjugated linoleic acid consumption during pregnancy and lactation influences growth and tissue composition in weaned pigs. J. Nutr. 130:2981-2989.
  6. Bergmeyer, H. U. 1974. Citrate lyase. In: (Ed. H. U. Bergmeyer), Methods of Enzymatic Analysis (Vol.1). Academic Press, New York and London. pp. 442-443.
  7. Crabtree, B., A. R. Leech and E. A. Newsholme. 1979. Measurement of enzyme activities in crude extract of tissues. In: (Ed. H. L. Kornberg, J. C. Metcalfe, D. H. Northcote, C. I. Pogson and K. F. Tipton), Techniques in Metabolic Researchpart 1. Elsevier/North-Holland Biomedical Press, Amsterdam, The Netherlands. B211:1-37.
  8. Duee, P. H., J. P. Pegorier, P. A. Quant, C. Herbin, C. Kohl and J. Girard. 1994. Hepatic ketogenesis in newborn pigs is limited by low mitochondrial 3-hydroxy-3methylglutary-CoA synthase activity. Biochem. J. 298:207-212.
  9. Gaiva, M. H. G., R. C. Couto, L. M. Oyama, G. E. C. Couto, V. L. F. Silverira, E. B. Riberio and C. M. O. Nascimento. 2001. Polyunsaturated fatty acid-rich diets: Effect on adipose tissue metabolism in rats. Br. J. Nutr. 86:371-377.
  10. Gibbons, G. F. 1990. Assembly and secretion of hepatic very-lowdensity lipoprotein. Biochem. J. 268:1-13. https://doi.org/10.1042/bj2680001
  11. Kritchevsky, D. 1988. Dietary fiber. Ann. Rev. Nutr. 8:301-328.
  12. Le Dividich, J., P. Herpin, J. Mourot and A. P. Colin. 1994. Effect of low fat colostrum on fat accretion and lipogenic enzymes in adipose tissue in the 1-day-old pig. Comp. Biochem. Physiol. 108:663-671.
  13. Lee, Y. B. and R. G. Kauffman. 1974. Cellularity and lipogenic enzyme activities of porcine intramuscular adipose tissue. J. Anim. Sci. 38:538-544.
  14. Leskanich, C. D. and R. C. Noble. 1999. The comparative roles of polyunsaturated fatty acids in pig neonatal development. Br. J. Nutr. 81:87-106.
  15. Leveille, G. A. 1970. Adipose tissue metabolism: influence of periodicity of eating and diet composition. Fed. Proc. 29:1294-1301.
  16. Lupton, J. R. 1995. Short-chain fatty acids and colon tumorigenesis: animal models. In: (Ed. J. H. Cummings and J. J. Rombeau), Physiological and Clinical Aspects of Shortchain Fatty Acids. Cambridge University Press, Great Britain. pp. 307-318.
  17. Macfarlane, G. T. and J. H. Cummings. 1991. The colonic flora, fermentation, and large bowel digestive function. In: (Ed. S. F. Phillips, J. H. Pemberton and R. G. Shorter), The Large Intestine, Physiology, Pathophysiology and Disease. Raven Press, New York, NY. pp. 51-92.
  18. Mourot, J., M. Kouba and P. Peiniau. 1995. Comparative study of in vitro lipogenesis in various adipose tissues in the growing domestic pig. Comp. Biochem. Physiol. 111B:379-384.
  19. Quant, P. A., P. K. Tubbs and M. D. Brand. 1989. Treatment of rats with glucagon or mannoheptulose increases mitochondrial 3-hydroxy-3-methylgutary-CoA synthase activity and decreases succinyl-CoA content in liver. Biochem. J. 262:159-164.
  20. Raju, J., D. Gupta, A. R. Rao and P. K. Yadava. 2001. Trigonella foenum graecum (fenugreek) seed power improves glucose homeostasis in alloxan diabetic rat tissues by reversing the altered glycolytic, gluconeogenic and lipogenic enzymes. Mol. Cell. Biochem. 224:45-51.
  21. Salway, J. G. 1994. Metabolism of glucose to fatty acids and triacylglycerol. In: (Ed. J. G. Salway), Metabolism at a Glance. Cambridge University Press, Great Britain. pp. 30-31.
  22. Stoldt, W. 1952. Verschlag zur Vereinheitlichung der Fettbestimmung in Lebensmitteln (Suggestion to standardize the determination of fat in food stuffs). Fette, Seifen, Anstrichmittel. 54:206-207.
  23. Stryer, L. 1995. Fatty acid metabolism. In: (Ed. L. Stryer), Biochemistry. 4th edition. Freeman and Company, New York. pp. 603-628.
  24. Thumelin, S., M. Forestier, J. Girard and J. P. Pegorier. 1993. Developmental changes in mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene expression in rat liver, intestine and kidney. Biochem. J. 292:493-496.
  25. Wang, J. F., B. B. Jensen, H. Jørgensen, D. F. Li and J. E. Lindberg. 2002. Ileal and total tract digestibility, and protein and fat balance in pigs fed rice with addition of potato starch, sugar beet pulp or wheat bran. Anim. Feed Sci. Tech. 102:125-136.
  26. Wang, J. F., D. F. Li, B. B. Jensen, K. Jakobsen, J. J. Xing, L. M. Gong and Y. H. Zhu. 2003. Effect of type and level of fiber on gastric microbial activity and short-chain fatty acid concentrations in gestating sows. Anim. Feed Sci. Tech. 104:95-110.
  27. Wise, E. M. and E. G. Ball. 1964. Malic enzyme and lipogenesis. Proc. Natl. Acad. Sci. USA 52:1255-1263.

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