Asian-Australasian Journal of Animal Sciences

  • 제16권5호
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  • Pages.732-737
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  • 2003
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Anti-idiotypic Antibodies against Bovine Growth Hormone

  • Verma, N.K. (Animal Biochemistry Division, National Dairy Research Institute) ;
  • Sodhi, R. (Animal Biochemistry Division, National Dairy Research Institute) ;
  • Rajput, Y.S. (Animal Biochemistry Division, National Dairy Research Institute)
  • 투고 : 2002.06.21
  • 심사 : 2002.11.21
  • 발행 : 2003.05.01
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초록

Anti-antibodies against three mouse monoclonal antibodies viz. IIB5D6, VIA6E8 and VIC1F9 (specific to bovine growth hormone) in rabbits have been generated and characterized. Ammonium sulfate fractionated and affinity-purified monoclonal antibodies were used for producing anti-antibodies. The generated anti-antibodies were against common as well as uncommon antigenic determinants present in mouse monoclonal antibodies. The raised anti-antibodies replaced [$I^125$ ]bGH bound to goat liver microsomes indicating production of anti-idiotypic antibodies against bovine growth hormone. These antibodies can have profound implications in vivo in lactating bovines for enhancing milk yield.

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참고문헌

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피인용 문헌

  1. Development and Characterization of a Novel Anti-idiotypic Monoclonal Antibody to Growth Hormone, Which Can Mimic Physiological Functions of Growth Hormone in Primary Porcine Hepatocytes vol.28, pp.4, 2015, https://doi.org/10.5713/ajas.14.0600