BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Regulation of the Gene Encoding Glutathione Synthetase from the Fission Yeast

  • Kim, Su-Jung (Division of Life Sciences, Kangwon National University) ;
  • Shin, Youn-Hee (Division of Life Sciences, Kangwon National University) ;
  • Kim, Kyung-Hoon (Division of Life Sciences, Kangwon National University) ;
  • Park, Eun-Hee (College of Pharmacy, Sookmyung Womens University) ;
  • Sa, Jae-Hoon (Department of Food and Drug Analysis, Kangwon Research Institute of Health and Environment) ;
  • Lim, Chang-Jin (Division of Life Sciences, Kangwon National University)
  • 투고 : 2002.11.09
  • 심사 : 2002.12.18
  • 발행 : 2003.05.31
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초록

The fission yeast cells that contained the cloned glutathione synthetase (GS) gene showed 1.4-fold higher glutathione (GSB) content and 1.9-fold higher GS activity than the cells without the cloned GS gene. Interestingly, $\gamma$-glutamylcysteine synthetase activity increased 2.1-fold in the S. pombe cells that contained the cloned GS gene. The S. pombe cells that harbored the multi copy-number plasmid pRGS49 (containing the cloned GS gene) showed a higher level of survival on solid media with cadmium chloride (1 mM) or mercuric chloride ($10\;{\mu}M$) than the cells that harbored the YEp357R vector. The 506 bp upstream sequence from the translational initiation point and N-terminal8 amino acid-coding region were fused into the promoteriess $\beta$-galactosidase gene of the shuttle vector YEp367R to generate the fusion plasmid pUGS39. Synthesis of $\beta$-galactosidase from the fusion plasmid pUGS39 was significantly enhanced by cadmium chloride and NO-generating S-nitroso-N-acetylpenicillamine (SNAP) and sodium nitroprusside (SN). It was also induced by L-buthionine-(S,R)-sulfoximine, a specific inhibitor of $\gamma$-glutamylcysteine synthetase (GCS). We also found that the expression of the S. pombe GS gene is regulated by the Atf1-Spc1-Wis1 signal pathway.

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과제정보

연구 과제 주관 기관 : Korea Research Foundation

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  3. Metabolic Control Analysis: A Tool for Designing Strategies to Manipulate Metabolic Pathways vol.2008, 2008, https://doi.org/10.1155/2008/597913
  4. Control of glutathione and phytochelatin synthesis under cadmium stress. Pathway modeling for plants vol.238, pp.4, 2006, https://doi.org/10.1016/j.jtbi.2005.07.003
  5. Disruption of redox homeostasis and induction of apoptosis by suppression of glutathione synthetase expression in a mammalian cell line vol.45, pp.9, 2011, https://doi.org/10.3109/10715762.2011.591392
  6. Glutathione plays a role in protecting leaves of Salvinia minima from Pb2+ damage associated with changes in the expression of SmGS genes and increased activity of GS vol.75, 2012, https://doi.org/10.1016/j.envexpbot.2011.09.001
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  9. The Gene Encoding γ-Glutamyl Transpeptidase II in the Fission Yeast Is Regulated by Oxidative and Metabolic Stress vol.38, pp.5, 2005, https://doi.org/10.5483/BMBRep.2005.38.5.609