Journal of Gastric Cancer
- Volume 3 Issue 3
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- Pages.128-133
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- 2003
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- 2093-582X(pISSN)
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- 2093-5641(eISSN)
Use of RT-PCR for CK20 and CEA mRNA to Detect of Micrometastasis in the Draining Venous Blood and the Peritoneum in Gastric Cancer Patients
위암에서 CK20과 CEA의 RT-PCR을 이용한 수술조작 전후 유출 정맥과 복막에서의 미세전이의 검출
- Joo Jai Kyun (Divisin of Gastroenterology Departments of Surgery, College of Medicine Chonnam National University Hospital) ;
- Lee Ji Hee (Divisin of Gastroenterology Departments of Surgery, College of Medicine Chonnam National University Hospital) ;
- Koh Yang Seok (Divisin of Gastroenterology Departments of Surgery, College of Medicine Chonnam National University Hospital) ;
- Kim Jung Chul (Divisin of Gastroenterology Departments of Surgery, College of Medicine Chonnam National University Hospital) ;
- Ryu Seong Yeob (Divisin of Gastroenterology Departments of Surgery, College of Medicine Chonnam National University Hospital) ;
- Kim Dong Yi (Divisin of Gastroenterology Departments of Surgery, College of Medicine Chonnam National University Hospital) ;
- Kim Young Jin (Divisin of Gastroenterology Departments of Surgery, College of Medicine Chonnam National University Hospital)
- 주재균 (전남대학교 의과대학 외과학교실, 위장관 외과) ;
- 이지희 (전남대학교 의과대학 외과학교실, 위장관 외과) ;
- 고양석 (전남대학교 의과대학 외과학교실, 위장관 외과) ;
- 김정철 (전남대학교 의과대학 외과학교실, 위장관 외과) ;
- 류성엽 (전남대학교 의과대학 외과학교실, 위장관 외과) ;
- 김동의 (전남대학교 의과대학 외과학교실, 위장관 외과) ;
- 김영진 (전남대학교 의과대학 외과학교실, 위장관 외과)
- Published : 2003.09.01
Abstract
Purpose: The benefits of the 'no-touch' isolation techniquethat is usually performed to prevent the circulation of tumor cells are not evident. The aim of this study was to determine whether the no-touch isolation technique for treating gastrointestinal cancers could prevent the circulation of tumor cells detected by reverse transcriptase polymerase chain reaction (RT-PCR). Matrials and Methods: By using RT-PCR to amplify mRNAs for two specific epithelial markers, carcinoembryonic antigen (CEA) and cytokeratin 20 (CK-20), we examined 34 gastric cancer patients who had been histologically diagnosed and 22 patients had undergone serosal and peritoneal brushing. Results: In 10 (
Keywords
- CK20 Gastric cancer;
- Micrometastasis;
- Reverse transcriptase polymerase chain reaction (RT-PCR);
- Carcinoembryonic antigen (CEA);
- Cytokeratin 20 (CK-20)
- 위암;
- 미세전이;
- 역전사중합효소연쇄반응;
- 암배아성 항원;