Journal of Gastric Cancer

The Korean Gastric Cancer Association (대한위암학회)
권호 표지

Abnormal Fragile Histidine Triad Gene Expression in Gastric Cancer

위선암에서 FHIT 유전자 발현이상의 임상적 의의

  • Lee, Moon-Soo (Departments of Surgery College of Medicine, Soonchunhyatng University) ;
  • Kim, Tae-Yun (Departments of Surgery College of Medicine, Soonchunhyatng University) ;
  • Cho, Gyu-Seok (Departments of Surgery College of Medicine, Soonchunhyatng University) ;
  • Chae-Man-Kyu (Departments of Surgery College of Medicine, Soonchunhyatng University) ;
  • Kim, Sung-Yong (Departments of Surgery College of Medicine, Soonchunhyatng University) ;
  • Baek-Moo-Jun (Departments of Surgery College of Medicine, Soonchunhyatng University) ;
  • Lee, Sang-Han (Departments of Biochemistry, College of Medicine, Soonchunhyatng University) ;
  • Park, Kyung-Kyu (Departments of Surgery College of Medicine, Soonchunhyatng University) ;
  • Kim, Chang-Ho (Departments of Surgery College of Medicine, Soonchunhyatng University) ;
  • Song-Ok-Pyung (Departments of Surgery College of Medicine, Soonchunhyatng University) ;
  • Cho, Moo-Sik (Departments of Surgery College of Medicine, Soonchunhyatng University)
  • 이문수 (순천향대학교 의과대학 천안병원 외과학교실) ;
  • 김태윤 (순천향대학교 의과대학 천안병원 외과학교실) ;
  • 조규석 (순천향대학교 의과대학 천안병원 외과학교실) ;
  • 채만규 (순천향대학교 의과대학 천안병원 외과학교실) ;
  • 김성용 (순천향대학교 의과대학 천안병원 외과학교실) ;
  • 백무준 (순천향대학교 의과대학 천안병원 외과학교실) ;
  • 이상한 (순천향대학교 의과대학 천안병원 생화학교실) ;
  • 박경규 (순천향대학교 의과대학 천안병원 외과학교실) ;
  • 김창호 (순천향대학교 의과대학 천안병원 외과학교실) ;
  • 송옥평 (순천향대학교 의과대학 천안병원 외과학교실) ;
  • 조무식 (순천향대학교 의과대학 천안병원 외과학교실)
  • Published : 2003.03.01
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Abstract

Purpose: Genomic alterations and abnormal expression of the fragile histidine triad (FHIT) gene in gastric cancer were examined to determine whether the FHIT gene is actually a frequent target for alteration during gastric carcinogenesis. Materials and Methods: To correlate DNA and RNA lesions of the FHIT gene with the effect on FHIT protein expression, in 40 gastric cancers, we investigated the FHIT gene for loss of heterozygisity (LOH), aberrant transcripts, and protein expression. Results: Allelic loss at D3S1300 was detected in 7 of 38 ($19\%$) informative cases. Aberrant transcripts were observed in 20 of 40 ($50\%$) cases. Significant reduction of FHIT protein expression was observed in 22 of 40 ($55\%$) cases. Aberrant FHIT transcription was shown to be associated with loss of FHIT protein expression. However, aberrent FHIT transcripts themselves were not associated with any clinicopathological parameters, such as age, sex, tumor site, or clinical stage. Moreover, there was no association between the presence of LOH at D3S1300 and the expression of aberrant FHIT transcripts. Conclusion: The high frequency of aberrant FHIT transcripts, the significant rate of LOH at D3S1300, and the altered expression of the FHIT protein indicate that alterations of the FHIT gene can play an important role in gastric carcinogenesis.

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