Journal of Gastric Cancer
- Volume 3 Issue 1
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- Pages.19-25
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- 2003
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- 2093-582X(pISSN)
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- 2093-5641(eISSN)
Expression of Survivin and HIAP-1 in Korean Gastric Cancers
한국인 위암에서 Survivin과 HIAP-1 유전자 발현
- Park Chan Jin (Departments of Surgery Keimyung University School of Medicine) ;
- Ryu Seung Wan (Departments of Surgery Keimyung University School of Medicine) ;
-
Kim In Hoo
(Departments of Surgery Keimyung University School of Medicine) ;
-
Baek Won-Ki
(Departments of Microbiology, Keimyung University School of Medicine) ;
- Suh Seong-Il (Departments of Microbiology, Keimyung University School of Medicine) ;
-
Suh Min-Ho
(Departments of Microbiology, Keimyung University School of Medicine) ;
- Sohn Soo Sang (Departments of Surgery Keimyung University School of Medicine)
- 박찬진 (계명대학교 의과대학 외과학교실) ;
- 류승완 (계명대학교 의과대학 외과학교실) ;
-
김인호
(계명대학교 의과대학 외과학교실) ;
-
백원기
(계명대학교 의과대학 미생물학교실) ;
- 서성일 (계명대학교 의과대학 미생물학교실) ;
-
서민호
(계명대학교 의과대학 미생물학교실) ;
- 손수상 (계명대학교 의과대학 외과학교실)
- Published : 2003.03.01
Abstract
Purpose: Dysregulation of apoptosis may attribute to development of cancer by abnormally prolonging cell viability with accumulation of transforming mutations. Survivin and HIAP (Human Inhibitors of Apoptosis)-1 were recently described as apoptosis inhibitors. Their pathogenic roles in gastric cancer are largely unknown. In the present study, we examined the expression of survivin and HIAP-1 in gastric cancer tissues and cell lines in order to elucidate the roles of survivin and HIAP-1 in the process of gastric carcinogenesis. Materials and Methods: Eight gastric cancer cell lines and five gastric cancer tissues were studied. The expression of survivin and HIAP-1 were evaluated by reverse transcription -polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blot. Results: Western blot and RT-PCR analysis revealed survivin and HIAP-1 expression in all gastric cancer cell lines. Increased expression of survivin and HIAP-1 were found in all cases of gastric cancer tissues compared to normal tissues by Western blot analysis. In immunohistochemical analysis tumor cells were stained with anti-survivin and anti-HIAP-1 antibodies. Cell cycle dependence of survivin expression was preserved in gastric cancer cell lines. Conclusion: The results indicate that increased expression of survivin and HIAP-1 genes may play an important role in gastric cancer.