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Korea Genome Organization (한국유전체학회)
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Genome Mapping of an Extreme Thermophile, Thermus caldophilus GK24

  • Park, Jong Hoon (Department of Biological Science, Sookmyung Woman's Univ., Genome Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Byung Chul (Glycobiology Laboratory and Korea Research Institute of Bioscience and Biotechnology) ;
  • Koch, Suk Hoon (Glycobiology Laboratory and Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Joong Soo (Glycobiology Laboratory and Korea Research Institute of Bioscience and Biotechnology) ;
  • Koh, Jeong Heon (Glycobiology Laboratory and Korea Research Institute of Bioscience and Biotechnology) ;
  • Yang, Moon Hee (Genome Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Yong Sung (Genome Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Cheorl Ho (Genome Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Myoung Hee (Genome Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwon, Suk Tae (Department of Genetic Engineering, SungKyunKwan Univ.) ;
  • Lee, Dae-Sil (Glycobiology Laboratory and Genome Center, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2003.09.01
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Abstract

Genome of an extreme thermophile, Thermus caldophilus GK24 has been analyzed to construct the genomic map. The genomic DNAs encapsulated in agarose gel were digested with SspI, EcoRI, SpeI, and HpaI restriction endonucleases, and then the resulting genomic DNA fragments were analyzed by pulsed-field gel electrophoresis. Its restriction map has been constructed by analyzing sizes of the restriction fragments obtained from both complete and partial digestions. The circular form of its genome was composed of about 1.98 Mbp and a megaplasmid. The genomic loci for the genes of xylose isomerase, thioredoxin, tRNA-16S rRNA, 23S rRNA, L5 ribosomal protein, ADP-glucose pyrophosphorylase, DNA-ligase, and Tca DNA polymerase were determined by both Southern hybridization and PCR.

Keywords

References

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