The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Production of Laccase by Fomitella fraxinea

Fomitella fraxinea에 의한 Laccase의 대량생산

  • Yoon, Jae-Don (Dept. Food Science and Technology, Yeungnam University) ;
  • Lee, Jong-Suk (Dept. Food Science and Technology, Yeungnam University) ;
  • Lee, Kyung-A (Dept. Food Science and Technology, Yeungnam University) ;
  • Chung, Min-Wook (Dept. Food Science and Technology, Yeungnam University) ;
  • Ha, Hyo-Cheol (Institute of Food & Culture, Pulmuone Co. Ltd.) ;
  • Lee, Jae-Sung (Dept. Food Science and Technology, Yeungnam University)
  • 윤재돈 (영남대학교 식품가공학과) ;
  • 이종숙 (영남대학교 식품가공학과) ;
  • 이경아 (영남대학교 식품가공학과) ;
  • 정민욱 (영남대학교 식품가공학과) ;
  • 하효철 ((주)풀무원 식문화 연구원) ;
  • 이재성 (영남대학교 식품가공학과)
  • Published : 2003.12.30
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Abstract

The production of laccase by Fomitella fraxinea was studied. The addition of minerals were necessary far laccase production by Fomitella fraxinea. Jar fermentor and Air-sparging fermentor performed high productivity In laccase activity by F. fraxinea. Laccase activity reached 3,540 in 8 days (Jar fermentor) and 3,100 in 6 days (Air-sparging fermentor) respectively.

담자균류 80여종에서 laccase 고생산 균주(Fomitella fraxinea; 장수버섯)를 선발하고, F. fraxinea를 이용한 리그닌 분해효소의 생산 및 효소의 대량생산 조건을 검토하였다. 먼저 배지 조성에 따른 생산성을 조사한 결과 Cu등의 무기물의 첨가 유무에 따라 생산되는 효소가 달랐다. 무기물이 첨가되지 않은 배지(Medium I)에서는 manganese peroxidase가 생산되었고, 무기물이 첨가된 배지(Medium II)에서는 laccase가 생산되었는데 플라스크 배양 18일째에는 5.43 U/ml, 20일째에는 5.56 U/ml의 효소활성이 나타났다. 배양방법에서 영양이 충분한 조건에서는 정치배양보다는 진탕배양(120 rpm)에서 효소의 활성이 높았다. 이 균주를 이용한 laccase 대량생산 실험에는 jar fermentor, balloon type bioreactor, air-sparging fermentor를 이용하였다. laccase는 jar fermentor와 air-sparging formentor에서 3,540 U/ml(8일), 3,100 U/ml(6일)의 생산성을 나타내었다. Balloon type bioreactor는 균사의 생장은 좋았지만 효소는 생산되지 않았다. F. fraxinea를 사용하여 air-sparging fermentor로 scale up하여 laccase의 대량생산을 할 수 있다고 판단된다.

Keywords

References

  1. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M.M. https://doi.org/10.1016/0003-2697(76)90527-3
  2. FEMS Microbiology Letter v.128 Efect of nitrogen and manganese on manganese peroxidase and laccase production by Lentinus edodes Buswell, J.A.;Cai, Y.;Chang, S.T. https://doi.org/10.1111/j.1574-6968.1995.tb07504.x
  3. The degradation of lignin by laccase from Pleurotus cornucopia (Pers.) Rolland Choi, D.H.
  4. Appl. Microbiol. Biotech. v.49 Laccase from the white rot fungus Trametes trogii Garzillo, A.M.V.;Colao, M.C. https://doi.org/10.1007/s002530051211
  5. Production of ligninolytic enzymes by the edible basidiomycete Pleurotus ostreatus Ha, H.C.
  6. Process Biochemistry v.37 Production of laccase and manganese peroxidase by Lentinus edodes on malt-containing byproduct of the brewing process Hatvani, N.;Mecs, I. https://doi.org/10.1016/S0032-9592(01)00236-9
  7. Apple. Environ. Microbiol. v.42 Nutritional regulation of lignin degradation by Phanerocheate chrysosporium Jeffries, T.W.;Choi, S.;Kirk, T.K.
  8. J. Kor. Cancer Association v.24 Antitumor components of the cultured mycelia of Calvatia craniformis Kim, B.K.;Kwun, J.Y.;Park, Y.I.
  9. Lignin Biodegration: Microbiology, Chemistry, and Potential Applications Kirk, T.K.;Higuchi, T.;Chang, H.M.
  10. Mokuzai Gakkaishi v.37 Alkyl-aryl cleavage of phenolic ${\beta}-o-4$ lignin substructure model compound by Mn(II)-peroxidase isolated from Pleurotus ostreatus Kofujita, H.;Asad, Y.;Kuwahara, M.
  11. J. Korean For. Soc. v.91 Submerged culture of Tricholoma matsutake mycelium by balloon type air bubble bioreactor Lee, W.Y.;Ahn, K.A.;Kwon, O.W.;Ka, K.H.;Kwon, Y.J.
  12. International Biodeterioration & Biodegradation v.46 Oxidation mechanism of ligninolytic enzymes involved in the degration of environmental pollutants Mester, T.;Tien, M. https://doi.org/10.1016/S0964-8305(00)00071-8
  13. Process Biochemistry v.100 Production of laccase by Trametes versicor in an airlift fermentor Rancano, G.;Lorenzo, M.;Molares, N.;Couto, S.R.;Sanroman, M.A.
  14. A study on production of ligninases by white rot fungi and biodegradation of xenobiotics Ryu, W.Y.
  15. Science v.221 Lignin-degrading enzyme from the hymenomycete Phanerocheate chrysosporium burds Tien, M.;Kirk, T.K. https://doi.org/10.1126/science.221.4611.661
  16. Proc. Natl. Acid Sci. USA v.81 Lignin degrading enzyme from Phanerocheate chrysosporium: purification, characterization and catalytic properties of a unique Hpz-requiring oxygenase Tien, M.;Kirk, T.K. https://doi.org/10.1073/pnas.81.8.2280

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