Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Protective effects Acanthopanax senticosus extracts on ethanol-induced apoptosis in TM3 mouse Leydig cells

오가피전탕액(五加皮煎湯液)이 에탄올에 의해 유발되는 TM3 mouse Leydig 세포주의 apoptosis에 미치는 영향

  • Kim Jong-Chul (Dept. of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Kim Hee-Taek (Dept. of Ophthamotolaryngology & Dermatosurgery, College of Oriental Medicine, Semyung University) ;
  • Kim Ho-Hyun (Dept. of Physiology, College of Oriental Medicine, Semyung University) ;
  • Kim Ee-Hwa (Dept. of Meridian & Acupoint, College of Oriental Medicine, Semyung University)
  • 권순범 (세명대학교 한의과대학 경혈학교실) ;
  • 김희택 (세명대학교 한의과대학 안.이비인후.피부과학교실) ;
  • 김호현 (세명대학교 한의과대학 생리학교실) ;
  • 김이화 (세명대학교 한의과대학 경혈학교실)
  • Published : 2003.03.27
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Abstract

목적: 오가피가 mouse testis에서 유래된 Leydig 세포주에서 에탄올에 의해 유발된 아폽토시스에 미치는 영향을 조사하였다. 방법: TM3 세포주에서의 아폽토시스 변화를 관찰하기 위해서 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI), DNA fragmentation assay, 및 reverse transcription-polymerase chain reaction (RT-PCR) 방법을 이용하였다. 결과: MTT assay를 이용하여 분석한 결과 농도에 따른 세포독성의 효과가 에탄올 투여부터 관찰되었다. 또한 오가피로 전처치하고 에탄올을 처치하였을 때 세포독성이 크게 감소되었다. DAPI staining에서 오가피 투여군은 에탄올 투여군에 비해서 fragmentation이 억제되었다. RT-PCR의 분석에 의하여 caspase-3 mRNA 발현이 오가피 투여군은 알코올 투여군보다 유의성있게 억제됨을 보여주었다. 결론: TM3 Leydig 세포주에서 에탄올에 의해 유발된 아폽토시스는 전형적인 세포사멸 형태를 나타내었다. 반면에 오가피 투여군은 에탄올에 의해서 유발된 아폽토시스에서 세포보호 효과가 있음이 확인되었다.

Keywords

References

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