한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

밤꽃(Castanea Crenata Flos.) 메탄올 추출물의 항산화 효과

Antioxidative Activities of Castanea Crenata Flos. Methanol Extracts

  • 최창숙 (호서대학교 자연과학부) ;
  • 송은승 (호서대학교 자연과학부) ;
  • 김장수 (호서대학교 벤처전문대학원 첨단기술, (주)수진바이오텍) ;
  • 강명화 (호서대학교 자연과학부, 호서대학교 벤처전문대학원 첨단기술)
  • Choi, Chang-Suk (Department of Food Science and Nutrition, Hoseo University) ;
  • Song, En-Sung (Department of Food Science and Nutrition, Hoseo University) ;
  • Kim, Jang-Su (Graduate School of Venture, Hoseo University, Su-Jin BioTech. Co. LTD) ;
  • Kang, Myung-Hwa (Department of Food Science and Nutrition, Hoseo University, Graduate School of Venture, Hoseo University)
  • 발행 : 2003.12.01
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초록

밤꽃의 항산화효과를 측정하기 위해 밤꽃의 수꽃을 수집하여 메탄올로 추출한 후 추출물의 항산화 효과를 측정하였다. 밤꽃 조추출물의 추출수율은 6.26% 였으며, 총 페놀함량은 0.199 mg/mL 인 것으로 나타났다. DPPH법으로 free radical 소거능을 측정한 결과 밤꽃 추출물이 17.22%로 기존의 항산화제에 비해서는 소거능이 다소 낮은 것으로 측정되었다. SOD 유사활성 측정은 밤꽃 추출물이 65.10%로 BHT(95.70%)와 quercetin(93.29%)에 비해 낮았지만, ascorbic acid(30.30%) 보다는 높은 활성을 나타내었다. Lecithin 산화저해율은 밤꽃 추출물이 51.45%, BHT(67.08%), quercetin(63.46%), ascorbic acid(23.60%), chlorogenic acid(48.76%), tannic acid(50.10%)로 나타났다. 이상의 연구결과 밤꽃 추출물이 DPPH free radical 소거능에서 기존 항산화제에 비해 낮은 활성을 나타냈지만, 시간경과에 따른 활성의 지속성과 50% 이상의 지질과산화 억제효과가 있어 천연항산화제로서의 가능성이 충분히 있는 것으로 추정된다.

The antioxidative activities of methanol extracts of chestnut flower (Castanea Crenata Flos.) were determinated in vitro using an experimental model system. Solid yield of chestnut flower extracts was 6.26% and total phenolic acid accounted for about 20% of the crude extract. The DPPH radical scavenging activity of methanol extracts prepared from chestnut flower was 17.22%. Although the DPPH radical scavenging activity of chestnut flower extracts was lower than that of other antioxidants, chestnut flower extracts showed continuous activity by time course. The SOD-like activities of methanol extracts prepared from chestnut flower were 65.10%, 95.70% in BHT, 93.29% in quercetin, and 30.30% in ascorbic acid. Chestnut flower extracts showed 51.45% inhibitory effect on peroxidation of egg yolk lecithin.

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