대한약침학회지 (Journal of Pharmacopuncture)

  • 제6권2호
  • /
  • Pages.149-158
  • /
  • 2003
  • /
  • 2093-6966(pISSN)
  • /
  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
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봉약침액(蜂藥針液)이 RAW 264.7 세포의 PLA2 및 Calcium Concentration에 미치는 영향(影響)

The Effects of Bee Venom on PLA2 and Calcium Concentration in Raw 264.7 Cells

  • 윤종일 (경원대학교 한의과대학 침구학교실) ;
  • 조현철 (경원대학교 한의과대학 한방재활의학과교실) ;
  • 심성용 (경원대학교 한의과대학 안이비인후피부과교실) ;
  • 김기현 (경원대학교 한의과대학 침구학교실)
  • Yun, Jong-Il (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-won University) ;
  • Jo, Hyun-Chul (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyung-won University) ;
  • Sim, Sung-Yong (Department of Oriental Medical Surgery, Ophthalmology & Otorhinolayngology, College of Oriental Medicine, Kyung-won University) ;
  • Kim, Kee-Hyun (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-won University)
  • 발행 : 2003.06.30
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초록

Objective : The purpose of this study was to investigate the effect of Bee Venom on the lipopolysaccharide, sodium nitroprusside and hydrogen peroxide induced expression phospholipase $A_2$ and calcium concentration in RAW 264.7 cells, a murine macrophage cell line. Method : The expression of phospholipase $A_2$ was determined by western blotting with corresponding antibodies, and the generation of intracellular calcium concentration was investigated by delta scan system in RAW 264.7 cells. Results : 1. Compared with control, expressions of lipopolysaccharide-induced phospholipase $A_2$ were decreased significantly by $1\;{\mu}g/{\mu}l$ of bee venom and decreased by 0.5, $5\;{\mu}g/{\mu}l$ of bee venom. 2. Compared with control, expressions of sodium nitroprusside-induced phospholipase $A_2$ were decreased significantly by $5\;{\mu}g/{\mu}l$ of bee venom but increased by 0.5, $5\;{\mu}g/{\mu}l$ of bee venom. 3. Compared with control, expressions of hydrogen peroxide-induced phospholipase $A_2$ were decreased significaltly by $1{\mu}g/{\mu}l$ of bee venom and decreased by $0.5\;{\mu}g/{\mu}l$ of bee venom but increased by $5\;{\mu}g/{\mu}l$ of bee venom. 4. Compared with control, lipopolysaccharide, sodium nitroprusside and hydrogen peroxide- induced intracellular calcium concentrations were decreased by 0.5, 1, $5\;{\mu}g/{\mu}l$ of bee venom and by indomethacin

키워드

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