Journal of Pharmaceutical Investigation

  • 제33권4호
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  • Pages.281-286
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  • 2003
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  • 2093-5552(pISSN)
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  • 2093-6214(eISSN)
한국약제학회 (The Korean Society of Pharmaceutical Sciences and Technology)
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골수 유래 기질 줄기세포의 탐식작용 매개성 케모카인 수용체 발현 연구

Expression of Chemokine Receptors Involved in Receptor-Mediated Endocytosis of Bone Marrow-Derived Stromal Stem Cells

  • 발행 : 2003.12.20
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초록

To design gene deliver systems which can deliver higher amounts of genes into stem cells, we studied the expression of receptors involved in the receptor-mediated endocytosis of bone marrow stromal stem cells. Bone marrow was isolated from ICR mice, and bone marrow stromal stem cells were isolated based on their plastic adherence property. Several culture conditions were screened for effective and continuous culture of marrow stromal stem cells. MesenCult medium was finally used to cultivate marrow stromal stem cells in vitro. As candidate receptors, various chemokine receptors were studied. Both bone marrow cells ad marrow-derived stromal stem cells showed expression of CC chemokine receptors (CCR) and CXC chemokine receptors (CXCR). Marrow stromal stem cells showed higher expression of CCR5 ad CXCR4 chemokine receptors as compared to other types of chemokine receptors. Moreover, though the expression of chemokine receptors generally decreased in most chemokine receptors with the cultivaton of marrow stromal stem cells, CCR5 and CXCR4 chemokine receptors retained the higher level of receptor expressions over prolonged periods. These results suggest that the ligands exhibiting specific binding to CCR5 or CXCR4 might be used to modify gene delivery systems for increased levels of receptor-mediated gene delivery into stromal stem cells.

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참고문헌

  1. D.G. Phinney, G. Kopen, R.L. Isaacson and D.J. Prockop, Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and differentiation, J. Cell Biochem., 72, 570-585 (1999) https://doi.org/10.1002/(SICI)1097-4644(19990315)72:4<570::AID-JCB12>3.0.CO;2-W
  2. W.J. Rombouts and R.E. Ploemacher, Primary murine MSC show highly efficient homing to the bone marrow but lose homing ability following culture, Leukemia, 17, 160-170 (2003) https://doi.org/10.1038/sj.leu.2402763
  3. P.D. Robbins and S.C. Ghivizzani, Viral vectors for gene therapy, Pharmacol. Ther., 80, 35-47 (1998) https://doi.org/10.1016/S0163-7258(98)00020-5
  4. T. Niidome and L. Huang, Gene therapy progress and prospects: nonviral vectors, Gene Ther., 9, 1647-1652 (2002) https://doi.org/10.1038/sj.gt.3301923
  5. N.W Lukacs, A.L. Miller and Hogaboam CM, Chemokine receptors in asthma: searching for the correct immune targets, J. Immunol., 171, 11-15 (2003) https://doi.org/10.4049/jimmunol.171.1.11
  6. K. Kedzierska, S.M. Crowe, S. Turville and A.L. Cunningham, The influence of cytokines, chemokines and their receptors on HIV-1 replication in monocytes and macrophages, Rev. Med. Virol., 13, 39-56 (2003) https://doi.org/10.1002/rmv.369
  7. S. Venkatesan, J.J. Rose, R. Lodge, P.M. Murphy and J.F Foley, Distinct mechanisms of agonist-induced endocytosis for human chemokine receptors CCR5 and CXCR4, Mol. BioI. Cell, 14, 3305-3324 (2003) https://doi.org/10.1091/mbc.E02-11-0714
  8. F.R. Fischer, L. Santambrogio, Y. Luo, M.A. Berman,W.W. Hancock and M.E. Dorf, Modulation of experimental autoimmune encephalomyelitis: effect of altered peptide ligand on chemokine and chemokine receptor expression, J. Neuroimmunol., 110, 195-208 (2000) https://doi.org/10.1016/S0165-5728(00)00351-9
  9. Y.K. Oh, J.P. Kim, H. Yoon, J.M. Kim, J.S. Yang and C.K. Kim, Prolonged organ retention and safety of plasmid DNA administered in polyethylenimine complexes, Gene Then., 8, 1587-1592 (2001) https://doi.org/10.1038/sj.gt.3301516
  10. D.E. Wright, E.P. Bowman, A.J. Wagers, E.C. Butcher and I.L. Weissman, Hematopoietic stem cells are uniquely selective in their migratory response to chemokines, J. Exp. Med., 195, 1145-1154 (2002) https://doi.org/10.1084/jem.20011284