한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제11권1호
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  • Pages.31-39
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  • 2003
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

바위솔 추출물의 항산화활성 및 암세포 증식억제

The activity of antioxidants and suppression of cancer cell proliferation in extracts of Orostachys japonicus A. Berger

  • 김충현 (안동대학교 자연과학대학 생명자원과학부) ;
  • 박재호 (안동대학교 자연과학대학 생명자원과학부) ;
  • 임종국 (안동대학교 자연과학대학 생명자원과학부) ;
  • 이건주 (안동대학교 자연과학대학 생명자원과학부) ;
  • 정규영 (안동대학교 자연과학대학 생명자원과학부) ;
  • 정형진 (안동대학교 자연과학대학 생명자원과학부)
  • Kim, Chung-Hyun (School of Bioresources, College of natural science, Andong National University) ;
  • Park, Jae-Ho (School of Bioresources, College of natural science, Andong National University) ;
  • Lim, Jong-Kuk (School of Bioresources, College of natural science, Andong National University) ;
  • Lee, Kon-Joo (School of Bioresources, College of natural science, Andong National University) ;
  • Chung, Gyu-Young (School of Bioresources, College of natural science, Andong National University) ;
  • Jeong, Hyung-Jin (School of Bioresources, College of natural science, Andong National University)
  • 발행 : 2003.03.31
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초록

바위솔의 추출물로부터 항산화 활성 및 동물 항암세포를 이용한 생물학적 특성을 조사 해 본 결과는 다음과 같다. 생육지별 DPPH 프리라디칼 소거 및 xanthine/xanthine oxidase 억제 활성비교에서 재배지에서 수확한 시료의 활성이 $6.07(IC_{50}:{\mu}g/m{\ell})$로 가장 높았다 생육시기별 DPPH 프리라디칼 소거 및 xanthine/xanthine oxidase 억제의 활성은 9월 수확물이 가장 활성이 높았다. 실리카겔 컬럼크로마토그래피를 이용한 항산화물질 분리결과에서 S-4 분획물의 DPPH와 xanthine oxidase 억제활성이 $5.02(IC_{50}\;:\;{\mu}g/m{\ell})$와 $6.18(IC_{50}:{\mu}g/m{\ell})$을 나타냄으로써 높은 분리효율를 보였다. 항산화활성이 가장 높았던 LH-4 분획물의 주요화합물은 GC/MS에 의하여 지방산, 폴리페놀화합물 및 페놀유도체로 동정되었으며 alpha-androsta-7,14-diene과 1,2,3-benzenetriol이 주 물질이었다. POD 및 SOD 활성은 생육지간에 재배지, 산, 바닷가 순으로, 생육시기간에는 시기가 늦을수록 높게 나타났다 SOD 동위효소의 밴드수는 전 생육지, 전 생육시기에서 공히 2개의 밴드가 나타났으나, 그 세기는 재배지와 9월의 수확물이 가장 높은 활성을 나타내었다. 정제된 LH-4 분획물은 HL-60 세포의 종양증식을 억제시켰으며 종양유발유전자와 IPTG로 콜로니 형성을 유도한 암세포(2-12 cells)에서 분획물 400ppm 처리와 negative control 처리간의 colony형성은 유의차가 없었다.

The antioxidative activities and biological properties in the EtOAc extracts and purified extracts of Orostachys japonicus were measured by assay of DPPH, xanthine/xanthine oxidase and mammalian cells(2-12). Scavenging of DPPH radical and inhibition of xanthine/xanthine oxidase of EtOAc extracts were showed the highest activity in the arable land and in September. The S-4 fraction showed the highest activity among the silica-gel column chromatography fractions. LH-4 fraction showed higher activity than the other fractionsins in assay of DPPH and xanthine/xanthine oxidase. Fatty acids and phenolic compounds were identified by GC/MS and main compounds were 1,2,3-benzenetriol, alpha-androsta-7,14-diene in LH-4 fraction. The activities of POD and SOD in samples havested on different habitats were high such as arable land> intermountain> seashore. That of POD and SOD in crude extracts of late stage were higher than early stage. Isozyme bands of crude extracts samplinged in all habitats and all growing stages showed two bands and the signal intensity showed strongly according to passage of growing stage. The purified extracts of LH-4 fraction showed excellent inhibition effect in proliferation of HL-60 cells and markedly suppressed colony formation in mouse fibroblast cells. Dose response between partially purified extracts(400ppm) and negative control did not produced statistically significant reduction in colony formation.

키워드

참고문헌

  1. Allen RD (1995) Dissection of oxidative stress tolerance using transgenic plants. Plant Physiol 107 : 1049-1054 https://doi.org/10.1104/pp.107.4.1049
  2. Alscher RG, Hess JL (1993) Antioxidants in higher plants. CRC Press. Boca Raton. 1-17
  3. Beauchamp C, Freidovich I (1971) Superoxide dismutase : Improved assays and an assay applicable to acrylarnide gels. Anal Biochem. 44 : 276-287 https://doi.org/10.1016/0003-2697(71)90370-8
  4. Beckman KB, Ames BN (1998) The free radical theory of aging matures. Physiol. Rev. 78 : 547-581 https://doi.org/10.1152/physrev.1998.78.2.547
  5. Szent-Cyorgyi A, Benthsanth A, Rusznyak S, (1936) Vitamin nature of flavones. Nature. 138 : 798 https://doi.org/10.1038/138798a0
  6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 72 : 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  7. Brodie AM (1994) Aromatase inhibitor in the treatment of breast cancer. J. steroid biochemistry and molecular biology. Volume 49. Issue4-6. p 281-287 https://doi.org/10.1016/0960-0760(94)90269-0
  8. Cavalieri EL, Rogan EG, Cremonesi. P, Devanesan PD (1998) Radical cations as precursors in the metabolic formation of quinones from benzo[a]pvrerie and 6fluorobenzo[a]pyrene. Fluoro substitution as a probe for one-electron oxidation in aromatic substrates. Biochemical Pharmacology. Volume 37. Issue 11. p 2173-2182. https://doi.org/10.1016/0006-2952(88)90578-3
  9. Cerutti PA, Trump BF (1991) Inflammation and oxidative stress in carcinogenesis. Cancer Cells. 3 : 1-7 https://doi.org/10.1016/S1535-6108(03)00002-3
  10. Chandler FR, Freeman L, Hooper SN (1979) Herbal remedies of the maritime indians. J. Ethnopharmacol, 1 : 49-68 https://doi.org/10.1016/0378-8741(79)90016-3
  11. Chang WS, Chang YR, Lu FJ, Chiang HC (1994) Inhibitory effects of phenolics on xanthine oxidase. Anticancer Res. 14 : 501-506
  12. Choe SY, Yang KH (1982) Toxicological studies of antioxidants butylated hyroxytoluene(BHT) and buthylated hydroxyanisole(BHA)(in Korean). Korean J. Food Sci. Technol. 14 : 283-288
  13. Cos P, Yang L, Calomme M, Hu JP, Cimanga K, Van Poel B, Pieters L, Vlietinck AJ, Berghe DV (1998) Structureactivity relationship and classification of flavonoids as inhibitors of xanthine oxidase and superoxidase scavengers. Journal of Natural Products. 61 : 71-76 https://doi.org/10.1021/np970237h
  14. Cotelle N, Bernier JL, Catteau JP, Pommery J, Wallet JC, Gaydou EM (1996) Antioxidant properties of hydroxyflavones. Free Radic. Biol, Med. 20 : 35-43 https://doi.org/10.1016/0891-5849(95)02014-4
  15. Diplock AT (1994) Mol. Aspects Med. 15 : 293-376 https://doi.org/10.1016/0098-2997(94)90005-1
  16. Enzo S, Antonietta M, Giovanni B (1998) The effect of benzenediols and benzenetriols on the nitrosation of propranolol depends on the position of hydroxyl groups on the benzene ring. Mutation Research. Volume. 398. Issue 1-2. P. 75-82 https://doi.org/10.1016/S0027-5107(97)00242-X
  17. Frankel A, Mills GB (1996) Peptide and lipid growth factors decrease cis-diamminedichloroplatinum-induced cell death in human ovarian cancer cells, Clinica Cancer Research: an Official Journal of the American Association for Cancer Research. Volume 2. Issue 8. p 1307-1313
  18. Fridovich I (1986) Biological effects of the superoxide radical. Arch. Biochem. Biophys, 247 : 1-11 https://doi.org/10.1016/0003-9861(86)90526-6
  19. Fukuta YT, Osawa M, Namiki, Ozaki T (1985) Studies on antioxidative substances in sesame seed. Agric, Biol. Chem. 49 : 301-306 https://doi.org/10.1271/bbb1961.49.301
  20. Goldbohm RA, Hertog MG, Brant HA, Van Poppel G, Van der Brandt PA (1996) Consumption of black tea and cancer risk: a prospective cohort study. J. Natl, Cancer Inst. 88 : 93-100 https://doi.org/10.1093/jnci/88.2.93
  21. Goodman Gilman A, Wall TW, Nies AS, Taylor P (1990) Pharmacological Basis of Therapeutics. eighth ed. p 674-681
  22. Halliwell B, Aruoma OI (1991) DNA damage by oxygenderived species Its mechanism and measurement in mammalian systems. FEBS Letters. Volume 281. Issues 1-2. p 9-19 https://doi.org/10.1016/0014-5793(91)80347-6
  23. Harman D (1956) Aging: a thory based on free radical and radiation chemistry. J. Gerontol. 11 : 298-300 https://doi.org/10.1093/geronj/11.3.298
  24. Hertog MG (1996) Epidemiological evidence on potential health properties of flavonoids. Proc. Nutr. Soc. 55 : 385-397 https://doi.org/10.1079/PNS19960037
  25. Hassan HM (1984) In Free Radicals in Molecular Biology, Aging, and Disease(D.Armstrong, R.S.Sohal, R.G.Cutler, and T.F. Slater, Eds.), Raven Press. New York. p 77
  26. Hong SJ (2000) Health potential of genistein against prostate diseases. Proc. Intl'l Symp Soybean & Health. p 11-25
  27. Hosoda K, Noguchi M (1990) Studies on the culivitation of Bupleurum falcatum L.(1), Effects of cultivation condition on theroot growth and saponin contents. Chem. Pharm. Bull. 38(2) : 436 https://doi.org/10.1248/cpb.38.436
  28. Huang MT, Smart RC, Wong CQ, Conney AH (1998) Inhibitory effect of curcumin, Chlorogenetic acid, caffeic acid and Ferulic acid on tumor promotion in mouse skin by l2-o-tetradecanoylphorbol l3-acetate. Cancer Res. 48 : 5941-5946
  29. Jeong HJ, Yi Lam, Lumen BO (2002) Barley Lunasin suppress ras-induced colony formation and inhibits core histone acetylation in mammalian cells. J. Agric. Food Chem. 50 : 5903-5908 https://doi.org/10.1021/jf0256945
  30. Kang TB, Liang NC (1997) Studies on the inhibitory effects of qurcetin on the growth of HL-60 Leukemia cells. Biochmistry Pharmacology. Volume 54. p 1013-1018 https://doi.org/10.1016/S0006-2952(97)00260-8
  31. Kim CM, SHin KM, An KD, Lee SK (1998) 중약대사전. 정담. (7) p 4042-4044
  32. Lee SK (1998) Evaluation of cancer chemopreventive activity mediated by antioxidants and modulators of tumor promotion. Phd thesis of university of illinois at Chicago. 52-54
  33. Liu HS, Scrable H, Villaret DB, Lieberman MA, Stambrook PJ (1992) Cancer Res. 52 : 983-989
  34. Liu HS, Chen CY, Lee CH, Chou YI (1998) Br. J. Cancer 77 : 1777-1786 https://doi.org/10.1038/bjc.1998.296
  35. McCord JM, Fridovich I (1969) Superoxide dismutase. An enzymic function for erythrocuprein (Hemocuprein). J. Biol. Chem. 244 : 6049-6055
  36. Nnnae IP, Long BJ, Ling YZ, Grigorvev DN, Brodie DM (2000) anti-tumor effects and phamacokinetic profile of 17( 5'-isoxazolyl)androsta-4,16-dien-3-one(L-39) in mice: an inhibitor of androgen synthesis. British J. Cancer. Volume 83. Issue 1. p 74-82 https://doi.org/10.1054/bjoc.2000.1136
  37. Ohmori Y, Ito M, Kishi M, Mizutani H, Katada T, Konishi H (1995) Antiallergic constituents from oolong tea stem. BioI. Pharm. Bull. 18 : 683-686 https://doi.org/10.1248/bpb.18.683
  38. Pitcher LH, Zilinskas BA (1996) Overexpression of copper/ zinc superoxide dismutase in the cytosol of transgenic tobacco confers partial resistance to ozoneinduced foliar nerosis. PInt Physiol. 110 : 583-588 https://doi.org/10.1104/pp.110.2.583
  39. Runeckless VC, Krupa SV (1994) The impact of UV-B radiation and ozone on terrestial vegetation. Environ Pollut. 83 : 191-213. https://doi.org/10.1016/0269-7491(94)90035-3
  40. Ryu SN, Lee BH (1998) Antitumor activity of crude sesaminol in sesame seed. Korean J. Crop. Sci. 43(3) : 168-171
  41. Sadzuka Y, Sugiyama A, Nozawa Y, Hirota S (1996) The effects of theanine, as a novel biochemical modulator, on the antitumor activity of adriamycin. Cancer Lett. 105 : 203-209 https://doi.org/10.1016/0304-3835(96)04282-6
  42. Scott G (1997) Antioxidants in science, Technology, Medicine and Nutrition. Albion, Chichester. UK
  43. Serafini M, Ghiselli A, Ferro-Luzz A (1996). In vivo antioxidant effect of green and black tea in man. Eur. J. Clin. Nutr. 50 : 28-32
  44. Shigenaga M, Hagen TM, Ames BN (1994) Oxidative damage and mitochondrial decay in aging. Proc. Natl. Acad. Sci. USA. 91 : 10771-10778. https://doi.org/10.1073/pnas.91.23.10771
  45. Smith RC, Reeves JC, Dage RC, Schnettler RA (1987) Antioxidant properties of 2-imidazolones and 2-imidazolthiones. Biochem. Pharmacol. 36 : 1457-1460 https://doi.org/10.1016/0006-2952(87)90110-9
  46. Stoner GD, Mykhtar H (1995) Polyphenols as cancer chemopreventive agents. J. Cell. Bio. Chem. 22 : 169-180
  47. Kang TB, Liang NC (1997) Studies on the inhibitory effects of qurcetin on the growth of HL-60 leukemia cells
  48. Vijaya K, Ananthan S, Nalini R (1995) Antibacterial effect of theaflavin, Polyphenon 60(Camellia Sinensis) ans Euphorbia hirta on Shigella SPP. J. Ethnopharmacol. 49 : 115-118. https://doi.org/10.1016/0378-8741(95)90039-X
  49. Yamashita K, Nohara Y, Katayama K, Namiki M (1992) Sesame seed ligans and $\alpha$-tocopherol act synergistically to produce vitamin E activity in rats. Nutrient Metabolism : 2440-2446