Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Anti-arthritic Effect of a New Diet-Supplement Containing Red Ginseng Extract and Glucosamine Complex

홍삼추출물과 글루코사민 복합제제의 관절염에 미치는 영향

  • Jeong, Choon-Sik (College of Pharmacy, Duksung Women's University) ;
  • Hyun, Jin-Ee (College of Pharmacy, Duksung Womens University) ;
  • Kang, Min-Hee (College of Pharmacy, Duksung Women's University) ;
  • Sim, Joon-Soo (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Son, Mi-Jin (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Jung, Sang-Hoon (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kim, Jong-Hoon (EE WOO YANGHAENG Co.) ;
  • Lee, Kwang-Seong (Insam Processing Center, National Agricultural Coopeartive Federation) ;
  • Kim, Yeong-Shik (Natural Products Research Institute, College of Pharmacy, Seoul National University)
  • 정춘식 (덕성여자대학교 약학대학) ;
  • 현진이 (덕성여자대학교 약학대학) ;
  • 강민희 (덕성여자대학교 약학대학) ;
  • 심준수 (서울대학교 약학대학/천연물과학연구소) ;
  • 손미진 (서울대학교 약학대학/천연물과학연구소) ;
  • 정상훈 (서울대학교 약학대학/천연물과학연구소) ;
  • 김종훈 (이우양행) ;
  • 이광승 (농협중앙회 고려인삼창) ;
  • 김영식 (서울대학교 약학대학/천연물과학연구소)
  • Published : 2003.12.30
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Abstract

We evaluated the anti-arthritic effect of a new diet-supplement product containing red ginseng, glucosamine, shark cartilage, ascorbic acid and manganese chloride for the relieving arthritic symptoms. Anti-inflammatory activities of the aqueous extract of red ginseng (250 and 500 mg/kg), glucosamine (240 mg/kg) and shark cartilage (240 mg/kg) were tested individually on vascular permeability and carrageenan-induced paw edema. Glucosamine and shark cartilage showed the inhibition of vascular permeability by 29.6 and 32.9%, respectively. Red ginseng (500 mg/kg) and shark cartilage showed the inhibition of carrageenan-induced paw edema at 0.5, 1, 2 and 3 hr. The supplement (red ginseng mixture: RGM) composed of red ginseng (43.5%), glucosamine (25.0%), shark cartilage (25.0%), ascorbic acid (5.0%) and manganese chloride (1.5%) was prepared and its inhibitory activities including vascular permeability and carrageenan-induced paw edema were comparable to anti-inflammatory drugs such as diclofenac and ibuprofen. It was also tested on adjuvant-induced arthritis in rats as one of chronic arthritic tests and Randall-Selitto assay as an analgesic test. RGM showed the inhibition against the swelling of rat paws induced by Mycobacterium tuberculosis at a dose of 1,500 mg/kg. Determination of cytokines of the sera sampled from arthritis-induced animals indicated that RGM increased the levels of $interferon-{\gamma}$ and interleukin-6, representing the immunostimulatory effect by red ginseng. RGM treatment moderately reduced the production of NO in RAW 264.7 cells in a dose-dependent manner. Taken together, these results support that RGM can be applicable for the improvement of arthritic as a new diet-supplement.

Keywords

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