Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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The Effect of Korean Mistletoe Extract M11C (Non-Lectin Components) on the Tumor Necrosis $Factor-{\alpha}$ Secretion from Mouse Splenic Macrophages and on the Inhibition of Sarcoma 180-Induced Tumor Growth in Mice

한국산 겨우살이 추출물 M11C(비렉틴 구성물질)의 생쥐 비장 대식세포로부터 Tumor Necrosis $Factor-{\alpha}$ 분비효과와 생쥐에 Sarcoma 180으로 유도된 육종암의 성장 억제효과

  • Sung, Ki-Tai (Department of Immunology, College of Medicine, Konkuk University and Department of Oriental Medicine, College of Oriental Medicine, Semyung University) ;
  • Kang, Tae-Bong (Department of Immunology, College of Medicine, Konkuk University and The Institute for Biomedical Research, Handong University) ;
  • Jun, Myung-Ha (Department of Immunology, College of Medicine, Konkuk University) ;
  • Chang, Sung-Ho (Department of Immunology, College of Medicine, Konkuk University) ;
  • Lee, Jun-Ho (Department of Immunology, College of Medicine, Konkuk University) ;
  • Kim, Jong-Bae (The Institute for Biomedical Research, Handong University) ;
  • Choi, Wahn-Soo (Department of Immunology, College of Medicine, Konkuk University) ;
  • Yoo, Yung-Choon (Department of Microbiology, College of Medicine, Konyang University) ;
  • Seong, Nak-Sul (National Crop Experiment Station, RDA) ;
  • Lee, Sung-Tae (Department of Orthopaedic Surgery, College of Medicine, Konkuk University) ;
  • Sung, Hyun-Jea (Department of Oriental Medicine, College of Oriental Medicine, Semyung University) ;
  • Her, Erk (Department of Immunology, College of Medicine, Kunkuk University)
  • 성기태 (건국대학교 의과대학 면역학교실 및 세명대학교 한의과대학 한의학과) ;
  • 강태봉 (건국대학교 의과대학 면역학교실 및 한동대학교 생의학 연구소) ;
  • 전명하 (건국대학교 의과대학 면역학교실) ;
  • 장성호 (건국대학교 의과대학 면역학교실) ;
  • 이준호 (건국대학교 의과대학 면역학교실) ;
  • 김종배 (한동대학교 생의학 연구소) ;
  • 최완수 (건국대학교 의과대학 면역학교실) ;
  • 유영춘 (건양대학교 의과대학 미생물학교실) ;
  • 성낙술 (농촌진흥청 작물시험장 특용작물과) ;
  • 이성태 (건국대학교 의과대학 정형외과학교실) ;
  • 성현제 (세명대학교 한의과대학 한의학과) ;
  • 허억 (건국대학교 의과대학 면역학교실)
  • Published : 2003.09.30
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Abstract

Korean mistletoe (Viscum album) extract has been found to posses immunostimulatory activity. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to know whether this extract might activate mouse splenic macrophages to produce tumor necrosis $factor-{\alpha}\;(TNF-{\alpha}$) and might play a role in anticancer. To know the effect of M11C on the production of $TNF-{\alpha}$, the splenic macrophages were treated by the M11C, and then collected the supernatant (M11C stimulated splenic macrophage-conditioned media; MSCM). MSCM was analyzed for the $TNF-{\alpha}$ secretion by means of ELISA and immunoblotting, and mRNA expression was analyzed by RT-PCR. The S-180 murine sarcoma model was established to know the effect of M11C on the inhibition of tumor growth. M11C had the effect of $TNF-{\alpha}$ production from splenic macrophages performed by ELISA technique. This ELISA data was reconfirmed by immunoblotting assay. The effects of M11C on the expression of $TNF-{\alpha}$ mRNA from the macrophages was also shown. M11C also had the inhibitory effect of S-180 tumor growth. These data suggest that Korean mistletoe extract M11C may be used for an immunomodulator.

Keywords

References

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