Comparison of Biochemical Components among Different Fodders-treated Antlers

각종 사료군의 투여에 따른 녹용 성분의 비교

  • Ha, Young-Wan (Natural Products Research Institute College of Pharmacy, Seoul National University) ;
  • Jeon, Byong-T. (Korea Nokyoung Research Center, Konkuk University) ;
  • Moon, Sang-H. (Korea Nokyoung Research Center, Konkuk University) ;
  • Kim, Yeong-Shik (Natural Products Research Institute College of Pharmacy, Seoul National University)
  • 하영완 (서울대학교 천연물과학연구소) ;
  • 전병태 (건국대학교 한국녹용연구센터) ;
  • 문상호 (건국대학교 한국녹용연구센터) ;
  • 김영식 (서울대학교 천연물과학연구소)
  • Published : 2003.03.30

Abstract

Antler has been used as one of the important traditional oriental medicines for many years. It contains many biochemical components including lipids, peptides, carbohydrates, and inorganic substances. The various biological activities of antler are being considered owing to such biochemical components. The purpose of this research is to compare the biochemical components of antlers after treatment of three different kinds of fodder. They are mulberry (group A), Lycii Fructus (group B) and the complex of herbs (group C). The chemical composition of each antler was determined in three sections (top, middle, and bottom) and compared with those of the control. The contents of sialic acid, uronic acid and glycosaminoglycans increased in the top antlers of the group B. Total lipids content increased in the top antlers of all groups (A, B, and C). The concentration of inorganic ions (Ca, Mg, and P) decreased in all groups. Fattyacid composition was also analyzed by GC-MS and expressed as percentage of total fatty acid concentration. The content of palmitic acid decreased in all groups. The content of 2-hydrox-yhexadecanoic acid, which has not been reported in aster, increased in the top antlers of all groups. These results suggest that the treatment of special fodder may affect the composition of the biochemical components of antlers.

Keywords

References

  1. Kim, J. G and Xiao, P. G. (1995) Traditional drugsof the east, 450-451. Young Lim Sa
  2. 신국현, 이은방, 김재현, 정명숙, 조성익(1989) 분말녹용의 약물활성 연구(I). 생약학회지 20: 180-187
  3. 용재익 (1964)實驗的 家兎貧血에 미치는 鹿茸投輿의 影響. 약학회지 8: 6-11
  4. Wang, B., Zhao, X., Qi., Yang, X., Kaneko, S., Hattori, M., Namba, T. and Nomura, Y. (1988) Stimulating effect of deer antler extract on protein synthesis in senescene-accelerated mice in vivo. Chem. Pharm. Bull. 36: 2593-2598 https://doi.org/10.1248/cpb.36.2593
  5. Wang, B., Zhao, X., Qi., Yang, X., Kaneko, S., Hattoii, M., Namba, T. and Nomura, Y. (1988) Effect of repeated administration of deer antler extract on biochemical changes related to aging in senescene- accelerated mice. Chem. Pharm. Bull. 36: 2587-2592 https://doi.org/10.1248/cpb.36.2587
  6. 허금, 최숙현, 이히빈, 정규찬, 고돈이(1960) 鹿茸에 關한 硏究(第2報): 鹿茸이 實驗用 白鼠의 成長에 미치는 影響에 對하여. 약학회지 5: 10-15
  7. 김영은, 이승기, 윤웅찬, 김정숙(1975) 녹용의 약효 성분에 관한 연구(I): 녹용, 녹각, 고래 코 연골, 상어 척수 연골의 화학조성에 관하여. 한국생화학회지 8: 89-107
  8. 김영은, 이승기, 유희자(1976) 녹용의 약효 성분에 관한 연구(II): 녹용의 Acid Mucopolysaccharide 성분에 관하여. 한국생화학회지 9: 153-164
  9. 김영은, 이승기, 이명희, 신승언(1976) 녹용의 약효 성분에 관한 연구(III): 녹용 및 Pantocrin의 지방산 조성에 관하여. 한국생화학회지 9: 215-236
  10. 김영은, 이승기, 이명희(1977) 녹용의 약효 성분에 관한 연구(IV): 녹용 벨殼 < 프로스타그란던의 검출. 한국생화학회지 10: 1-12
  11. 김영은, 임동구, 심승언(1977) 녹용의 약효 성분에 관한 연구(V): 녹용 및 판토크린의 당지질과 인지질의 조성에 관하여. 한국생화학회지 10: 153-164
  12. Scott, J. E and Hughes, E. W. (1981) Chondroitin sulfate from fossilized antlers. Nature. 291: 580-581 https://doi.org/10.1038/291580a0
  13. Sunwoo, H. H., Nakano, T., Hudson, R. J., Sim, J. S. (1998) Isolation, characterization and localization of glycosaminoglycans in growing antlers of wapiti (Cervus etaphus). Comp Biochem Physio B. 120: 273-283 https://doi.org/10.1016/S0305-0491(98)10017-2
  14. Pathak, N. N., Pattanaik, A. K., Patra, R. C., Arora, B. M. (2001) Mineral composition of antlers of three deer species reared in capdvity. Small Ruminant Res. 42: 61-65 https://doi.org/10.1016/S0921-4488(01)00218-8
  15. Sunwoo, H. H., Nakano, T., Hudson, R. J. and Sim, J. S. (1995) Chemical Composition of Antlers from wapiti(Cervus etaphus). J. Agric. Food Chem. 43: 2846-2849 https://doi.org/10.1021/jf00059a014
  16. 신국현,임순성,정하숙,백인범(1999)꽃사슴 녹용의 생화학적성분의 조성 및 함량. 생약학회지 30: 314-319
  17. 전병태, 김경훈, 김운식 , 이상무, 김창원(1995) 사료급여형태를 중심으로 한 충북지역 사슴사육 실태조사. 韓營飼誌19: 235-241
  18. Kwak, W. S., Kim, K. H., Kim, C. W., Jeon, B. T and Lee, S. M (1994) Deer farming in Korea : On-farm survey in Kyung-kee and Chung-Buk provines. Asia-Austr. J. Anim. Sci. 7: 347-355
  19. William Horwitz (2000) Offcial Methods of Analysis of AOAC International. 2: Ch4, 40-41. AOAC International.Maryland, USA
  20. Christie, W. W. (1976) Lipids Analysis. Pergamon Press. New York
  21. Christie, W. W. (1990) Lipid Technolgy. 2: 48-49
  22. Christie, W. W. (1995) Are methyl esters of fatty acids the best choice for gas chromatographic analysis? Lipid Technology. 7: 64-66
  23. Scott, J. E. (1960) Aliphatic ammonium salts in the assay of acidic polysaccharides from tissues. Methods Biochem. Anal. 8: 145-197
  24. Kosakai, M., Yoshizawa, Z. (1979) A partial modification of the cartilage method of Bitter and Muir for quantitation of hexuronic acids. Anal. Biochem. 93: 295-298 https://doi.org/10.1016/S0003-2697(79)80154-2
  25. Farndale, R. W., Sayers, C. A., Barett, A. J. (1982) A direct spectrophotometric assay for sulfated glycosaminoglycans in cartilage cultures. Connect. Tiss. Res. 9: 247-248 https://doi.org/10.3109/03008208209160269
  26. Warren, L. (1959) The thiobarbituric acid assay of sialic acids. J. Biol. Chem. 234: 1971-1975
  27. Banks W. J. (1974) The ossification process of the developing ander in the white-tailed deer (Odocoileus virginianus). Calcif Tiss Res. 14: 257-274 https://doi.org/10.1007/BF02060300
  28. Dean D. D., Schwartz, Z., Bonewald, L., Muniz O. E., Morales, S., Gomez, R., Brooks, B. P., Qiao, M., Howell D. S., Boyan, B. D. (1994) Matrix vesicles produced by oste oblast-like cells in culture become signiScantly enriched in proteoglycan-degrading metalloproteinases after addition of b-glycerophosphate and ascorbic acid. Calcif Tissue Int. 54: 399-40 https://doi.org/10.1007/BF00305527
  29. Hakomori S (2000) Traveling for the glyco-sphingolipid path. GIycoconj. J. 17: 627-647 https://doi.org/10.1023/A:1011086929064